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Lysate of Probiotic Lactobacillus plantarum K8 Modulate the Mucosal Inflammatory System in Dextran Sulfate Sodium-induced Colitic Rats
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 Title & Authors
Lysate of Probiotic Lactobacillus plantarum K8 Modulate the Mucosal Inflammatory System in Dextran Sulfate Sodium-induced Colitic Rats
Ahn, Young-Sook; Park, Min Young; Shin, Jae-Ho; Kim, Ji Yeon; Kwon, Oran;
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Inflammatory bowel disease (IBD) is caused by dysregulation of colon mucosal immunity and mucosal epithelial barrier function. Recent studies have reported that lipoteichoic acid (LTA) from Lactobacillus plantarum K8 reduces excessive production of pro-inflammatory cytokine. In this study, we investigated the preventive effects of lysate of Lb. plantarum K8 in dextran sulfate sodium (DSS)-induced colitis. Male Sprague-Dawley rats were orally pretreated with lysate of Lb. plantarum K8 (low dose or high dose) or live Lb. plantarum K8 prior to the induction of colitis using 4% DSS. Disease progression was monitored by assessment of disease activity index (DAI). Histological changes of colonic tissues were evaluated by hematoxylin and eosin (HE) staining. Tumor necrosis factor-alpha (TNF-), interleukin-6 (IL-6) levels were measured using enzyme-linked immunosorbent assay (ELISA). The colon mRNA expressions of TNF-, IL-6, and toll like receptor-2 (TLR-2) were examined by quantitative real-time-transcription polymerase chain reaction (qPCR). Lysate of Lb. plantarum K8 suppressed colon shortening, edema, mucosal damage, and the loss of DSS-induced crypts. The groups that received lysate of Lb. plantarum K8 exhibited significantly decreased levels of the pro-inflammatory cytokines TNF- and IL-6 in the colon. Interestingly, colonic expression of toll like receptor-2 mRNA in the high-dose lysate of Lb. plantarum K8 group increased significantly. Our study demonstrates the protective effects of oral lysate of Lb. plantarum K8 administration on DSS-induced colitis via the modulation of pro-inflammatory mediators of the mucosal immune system.
colitis;lysate of Lactobacillus plantarum K8;pro-inflammatory cytokines;toll-like receptor 2;
 Cited by
Adams, C. A. (2010) The probiotic paradox: live and dead cells are biological response modifiers. Nutr. Res. Rev. 23, 37-46.

Cario, E. (2008) Barrier-protective function of intestinal epithelial Toll-like receptor 2. Mucosal. Immunol. 1, S62-S66. crossref(new window)

Cario, E., Gerken, G., and Podolsky, D. K. (2007) Toll-like receptor 2 controls mucosal inflammation by regulating epithelial barrier function. Gastroenterol. 132, 1359-1374. crossref(new window)

Carvalho, R. and Hyams., J. S. (2007) Diagnosis and management of inflammatory bowel disease in children. Semin. Pediatr. Surg. 16, 164-171.

Chen, Y. P., Hsiao, P. J. Hong, W. S. Dai, T. Y., and Chen, M. J. (2012) Lactobacillus kefiranofaciens M1 isolated from milk kefir grains ameliorates experimental colitis in vitro and in vivo. J. Dairy Sci. 95, 63-74. crossref(new window)

Cooper, H. S., Murthy, S. N., Shah, R. S., and Sedergran, D. J. (1993) Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab. Invest. 69, 238-249.

Fujiki, T., Hirose, Y., Yamamoto, Y., and Murosaki, S. (2012) Enhanced immunomodulatory activity and stability in simulated digestive juices of Lactobacillus plantarum L-137 by heat treatment. Biosci. Biotechnol. Biochem. 76, 918-922. crossref(new window)

Geier, M. S., Butler, R. N., Giffard, P. M., and Howarth, G. S. (2007) Lactobacillus fermentum BR11, a potential new probiotic, alleviates symptoms of colitis induced by dextran sulfate sodium (DSS) in rats. Int. J. Food Microbiol. 114, 267-274. crossref(new window)

Han, S. H., Kim, J. H., Martin, M., Michalek, S. M., and Nahm, M. H. (2003) Pneumococcal lipoteichoic acid (LTA) is not as potent as staphylococcal LTA in stimulating Toll-like receptor 2. Infect. Immun. 71, 5541-5548. crossref(new window)

Kang, S. S., Ryu, Y. H., Baik, J. E., Yun, C. H., Lee, K., Chung, D. K., and Han, S. H. (2011) Lipoteichoic acid from Lactobacillus plantarum induces nitric oxide production in the presence of interferron-gamma in murine macrophages. Mol. Immunol. 48, 2170-2177. crossref(new window)

Kataria, J., Li, N., Wynn, J. L., and Neu, J. (2009) Probiotic microbes: do they need to be alive to be beneficial? Nutr. Rev. 67, 546-550. crossref(new window)

Kim, H. G., Lee, S. Y., Kim, N. R., Lee, H. Y., Ko, M. Y., Jung, B. J., Kim, C. M., Lee, J. M., Park, J. H., Han, S. H., and Chung, D. K. (2011) Lactobacillus plantarum lipoteichoic acid down-regulated Shigella flexneri peptidoglycan-induced inflammation. Mol. Immunol. 48, 382-391. crossref(new window)

Li, X., Jiang, S., and Tapping, R. I. (2010) Toll-like receptor signaling in cell proliferation and survival. Cytokine. 49, 1-9. crossref(new window)

Mayhew, T. M. and Carson, F. L. (1989) Mechanisms of adaptation in rat small intestine: regional differences in quantitative morphology during normal growth and experimental hypertrophy. J. Anat. 164, 189-200.

McGuckin, M. A., Eri, R., Simms, L. A., Florin, T. H., and Radford-Smith, G. (2009) Intestinal barrier dysfunction in inflammatory bowel diseases. Inflamm. Bowel. Dis. 15, 100-113. crossref(new window)

Mileti, E., Matteoli, G., Iliev, I. D., and Rescigno. M. (2009) Comparison of the immunomodulatory properties of three probiotic strains of Lactobacilli using complex culture systems: prediction for in vivo efficacy. PLoS One 4, e7056. crossref(new window)

Neuman, M. G. (2007) Immune dysfunction in inflammatory bowel disease. Transl. Res. 149, 173-186. crossref(new window)

Neuman, M. G. and Nanau, R. M. (2012) Inflammatory bowel disease: role of diet, microbiota, life style. Transl. Res. 160, 29-44. crossref(new window)

Papadakis, K. A. and Targan, S. R. (2000) Role of cytokines in the pathogenesis of inflammatory bowel disease. Ann. Rev. Med. 51, 289-298. crossref(new window)

Podolsky, D. K. (1991) Inflammatory bowel disease (1). N. Engl. J. Med. 325, 928-937. crossref(new window)

Podolsky, D. K. (2002) Inflammatory bowel disease. N. Engl. J. Med. 347, 417-429. crossref(new window)

Rakoff-Nahoum, S., Paglino, J., Eslami-Varzaneh, F., Edberg, S., and Medzhitov, R. (2004) Recognition of commensal microflora by toll-like receptors is required for intestinal homeostasis. Cell 118, 229-241. crossref(new window)

Ryu, Y. H., Baik, J. E., Yang, J. S., Kang, S. S., Im, J., Yun, C. H., Kim, D. W., Lee, K., Chung, D. K., Ju, H. R., and Han, S. H. (2009) Differential immunostimulatory effects of Grampositive bacteria due to their lipoteichoic acids. Int. Immunopharmacol. 9, 127-133. crossref(new window)

Sartor, R. B. (2006) Mechanisms of disease: pathogenesis of Crohn's disease and ulcerative colitis. Nat. Clin. Pract. Gastroenterol. Hepatol. 3, 390-407. crossref(new window)

Suzuki, T. (2013) Regulation of intestinal epithelial permeability by tight junctions. Cell Mol. Life Sci. 70, 631-659. crossref(new window)

Triantafillidis, J. K., Merikas, E., and Georgopoulos, F. (2011) Current and emerging drugs for the treatment of inflammatory bowel disease. Drug Des. Devel. Ther. 5, 185-210.

Veckman, V., Miettinen, M., Pirhonen, J., Siren, J., Matikainen, S., and Julkunen, I. (2004) Streptococcus pyogenes and Lactobacillus rhamnosus differentially induce maturation and production of Th1-type cytokines and chemokines in human monocyte-derived dendritic cells. J. Leukoc. Biol. 75, 764-771.

Yuki, T., Yoshida, H., Akazawa, Y., Komiya, A., Sugiyama, Y., and Inoue, S. (2011) Activation of TLR2 enhances tight junction barrier in epidermal keratinocytes. J. Immunol. 187, 3230-3237. crossref(new window)

Zakostelska, Z., Kverka, M., Klimesova, K., Rossmann, P., Mrazek, J., Kopecny, J., Hornova, M., Srutkova, D., Hudcovic, T., Ridl, J., and Tlaskalova-Hogenova, H. (2011) Lysate of probiotic Lactobacillus casei DN-114 001 ameliorates colitis by strengthening the gut barrier function and changing the gut microenvironment. PLoS One 6, e27961. crossref(new window)

Zhou, Y. K., Qin, H. L., Zhang, M., Shen, T. Y., Chen, H. Q., Ma, Y. L., Chu, Z. X., Zhang, P., and Liu, Z. H. (2012) Effects of Lactobacillus plantarum on gut barrier function in experimental obstructive jaundice. World J. Gastroenterol. 18, 3977-3991. crossref(new window)