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Application of Response Surface Methodology (RSM) for Optimization of Anti-Obesity Effect in Fermented Milk by Lactobacillus plantarum Q180
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 Title & Authors
Application of Response Surface Methodology (RSM) for Optimization of Anti-Obesity Effect in Fermented Milk by Lactobacillus plantarum Q180
Park, Sun-Young; Cho, Seong-A; Lim, Sang-Dong;
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Obesity, a condition in which an abnormally large amount of fat is stored in adipose tissue, causing an increase in body weight, has become a major public health concern worldwide. The purpose of this study was to optimize the process for fermented milk for the production of a functional product with an anti-obesity effect by using Lactobacillus plantarum Q180 isolated from human feces. We used a 3-factor, 3-level central composite design (CCD) combined with the response surface methodology (RSM). Concentration of skim milk powder (%, ), incubation temperature (, ), and incubation time (h, ) were used as the independent factors, whereas pH (pH, ), anti-lipase activity (%, ) and anti-adipogenetic activity (%, ) were used as the dependent factors. The optimal conditions of fermented milk for the highest anti-lipase and anti-adipogenetic activity with pH 4.4 were the 9.5% of skim milk powder, of incubation temperature, 28 h of incubation time. In the fermentation condition, the predicted values of pH, anti-lipase activity and anti-adipogenetic activity were 4.47, 55.55, and 20.48%, respectively. However, the actual values of pH, anti-lipase activity and anti-adipogenetic activity were 4.50, 52.86, and 19.25%, respectively. These results demonstrate that 9.5% of skim milk powder and incubation at for 28 h were the optimum conditions for producing functional fermented milk with an anti-obesity effect.
Lactobacillus plantarum;optimization;anti-lipase activity;anti-adipogenetic activity;
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Hypolipidemic, antiobesity and cardioprotective effects of sardinelle meat flour and its hydrolysates in high-fat and fructose diet fed Wistar rats, Life Sciences, 2017, 176, 54  crossref(new windwow)
Bezerra, M. A., Santelli, R. E., Oliveira, E. P., Villar, L. S., and Escaleira, L. A. (2008) Response surgace methodology (RSM) as a tool for optimization in analytical chemistry. Talanta 76, 965-977. crossref(new window)

Box, G. E. P., Hunter, W. G., and Hunter, J. S. (1978) Statistics for experimenters. John Wiley and Sons, New York, 341-418.

Box, G. E. P. and Wilson, K. B. (1951) On the experimental attainment of optimum condition. J. Roy. Statist. Soc. Ser. B. 13, 1-38.

Choi, S. S., Kim, Y., Han, K. S., You, S., Oh, S., and Kim, S. H. (2006) Effects of Lactobacillus strains on cancer cell proliferation and oxidative stress in vitro. Lett. Appl. Microbiol. 42, 452-458. crossref(new window)

Hemati, N., Ross, S. E., Erickson, R. L, Groblewski, G. E., and MacDuygald, O. A. (1997) Signaling pathways through which insulin regulates CCAAT/enhancer binding protein-$\alpha$ (C/EBP-$\alpha$) phosphorylation and gene expression in 3T3-L1 adipocytes. Forrelation with CLUT4 gene expression. J. Biol. Chem. 272, 25913-25919. crossref(new window)

Kadooka, Y., Sato, M., Imaizumi, K., Ogawa, A., Ikuyama, K., Akai, Y., Okano, M., Kagoshima, M., and Tsuchida, T. (2010) Regulation of abdominal adiposity by probiotics (Lactobacillus gasseri SBT2055) in adults with obese tendencies in a randomized controlled trial. Eur. J. Clin. Nutr. 64, 636-643. crossref(new window)

Karahan, A. G., Kilic, G. B., Kart, A., Aloglu, H. S., Oner, Z., Aydemir, S., Erkus, O., and Harsa, S. (2010) Genotypic identification or some lactic acid bacteria by amplified fragment length polymorphism analysis and investigation of their potential usage as starter culture combinations in Beyaz cheese manufacture. J. Dairy Sci. 93, 1-11. crossref(new window)

Komatsuzaki, N., Shima, J., Kawamoto, S., Momose, H., and Kimura, T. (2005) Production of $\gamma$-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol. 22, 497-504. crossref(new window)

Lee, Y. P., Chung G. H., and Rhee, J. S. (1993) Purification and characterization of Pseudomonas fluorescens SIK W1 lipase expressed in Escherichia coli. Biochim. Biophys. Acta. 1169, 156-164. crossref(new window)

Leroy, F. and Vuyst, L. D. (2004) Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci. Technol. 15, 17-78.

Mosmann T. (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 62, 55-63.

Park, D. Y., Ahn, Y. T., Huh, C. S., Jeon, S. M., and Choi, M. S. (2011) The inhibitory effect of Lactobacillus plantarum KY1032 cell extract on the adipogenesis of 3T3-L1 cells. J. Med. Food. 10, 670-675.

Park, S. Y., Cho, S. A., Kim, S. H., and Lim, S. D. (2014) Physiological characteristics and anti-obesity effect of Lactobacillus plantarum Q180 isolated from feces. Korean J. Food Sci. An. 34, 645-653. crossref(new window)

Ramirez-Zacarias, J. L., Castro-Munozledo, F., and Kuri-Harcuch, W. (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochem. 97, 493-497. crossref(new window)

Salminen, M. K., Tynkkynen, S., Rautelin, H., Saxelin, M., Vaara, M., Ruutu, P., Sarna, S., Valtonen, V., and Jarvinen, A. (2002) Lactobacillus bacteremia during a rapid increase in probiotic use of Lactobacillus rhamnosus GG in Finland. Clin. Infect. Dis. 35, 1155-1160. crossref(new window)

Takemura, N., Okubo, T., and Sonoyama, K. (2010) Lactobacillus plantarum strain No. 14 reduces adipocyte size in mice fed high-fat diet. Exp. Biol. Med. 235, 849-856. crossref(new window)

Tanida, M., Shen, J., Maeda, K., Horii, Y., Yamano, T., Fukushima, Y., and Nagai, K. (2008) High-fat diet-induced obesity is attenuated by probiotic strain Lactobacillus paracasei ST11 (NCC2461) in rats. Obes. Res. Clin. Pract. 2, 159-169. crossref(new window)

Yanovski, S. Z. and Yanovski, J. A. (2002) Obesity. N. Engl. J. Med. 346, 591-602. crossref(new window)