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Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates
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 Title & Authors
Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates
Kim, Dongwook; Kim, Hee-Jin; Chae, Hyun-Seok; Park, Nam-Gun; Kim, Young-Boong; Jang, Aera;
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This study focused on the anti-oxidative and collagenase- and elastase inhibition effects of low molecular weight peptides (LMP) from commercial Jeju horse leg bone hydrolysates (JHLB) on pancreatin, via enzymatic hydrolysis. Cell viability of dermal fibroblasts exposed to UVB radiation upon treatment with LMP from JHLB was evaluated. Determination of the antioxidant activity of various concentrations of LMP from JHLB were carried out by assessing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethybenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). The DPPH radical scavenging activity of LMP from JHLB (20 mg/mL) was 92.21% and ABTS radical scavenging activity (15 mg/mL) was 99.50%. FRAP activity (30 mg/mL) was and ORAC activity (1 mg/mL) was . The anti-wrinkle potential was assessed by evaluating the elastase- and collagenase inhibition potential of these LMP. We found that 200 mg/mL of LMP from JHLB inhibited elastase activity by 41.32%, and 100 mg/mL of LMP from JHLB inhibited collagenase activity by 91.32%. The cell viability of untreated HS68 human dermal fibroblasts was 45% when exposed to a UVB radiation dose of . After 24 h of incubation with LMP from JHLB, the cell viability increased to 60%. These results indicate that LMP from JHLB has potential utility as an anti-oxidant and anti-wrinkle agent in the food and cosmetic industry. Additional in vivo tests should be carried out to further characterize these potential benefits.
horse leg bone;hydrolysates;collagenase;elastase;anti-oxidation;
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Functionality of porcine skin hydrolysates produced by hydrothermal processing for liposomal delivery system, Journal of Food Biochemistry, 2017, 01458884, e12464  crossref(new windwow)
Alvarez-Suarez, J. M., Giampieri, F., Gonzalez-Paramas, A. M., Damiani, E., Astolfi, P., Martinez-Sanchez, G., Bompadre, S., Quiles, J. L., Santos-Buelga, C., and Battino, M. (2012) Phenolics from monofloral honeys protect human erythrocyte membranes against oxidative damage. Food Chem. Toxicol. 50, 1508-1516. crossref(new window)

Benzie, I. F. and Strain, J. J. (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal. Biochem. 239, 70-76. crossref(new window)

Blois, M. S. (1958) Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1200. crossref(new window)

Cannell, R. J. P., Kellan, S. J., Owsianks, A. M., Walker, J. M. (1988) Results of a large scale scrren of microalgae for the production of protease inhibitors. Planta Med. 54, 10-14. crossref(new window)

Cao, G. and Prior, R. L. (1999) Measurement of oxygen radical absorbance capacity in biological samples. Methods Enzymol. 299, 50-62. crossref(new window)

Cheon, S. J., Jang, M. J., Jang, Y. A., Choi, E. Y., Jun, D. H., Kim, Y. H., Cho, W. A., Jeong, Y. S., Kwon, H, B., Kim, T. H., Choi, K. L., Do, J. R., Lee, C. E. and Lee J. T. (2008) Antiwrinkle effect of cambodian Phellinus linteus extracts. J. Life Sci. 18, 1718-1722. crossref(new window)

Chiu, A. E., Chan, J. L., Kern, D. G., Kohler, S., Rehmus, W. E., and Kimball, A. B. (2005) Double-blinded, placebocontrolled trial of green tea extracts in the clinical and histological appearance of photoaging skin. Dermatol. Surg. 31, 855-859.

Farage, M. A., Miller, K. W., Elsner, P., and Maibach, H. I. (2008) Intrinsic and extrinsic factors in skin ageing: a review. Inter. J. Cosmetic Sci. 30, 87-95. crossref(new window)

Gillespie, K. M., Chae, J. M., and Ainsworth, E. A. (2007) Rapid measurement of total antioxidant capacity in plants. Nature Protocols 2, 867-870. crossref(new window)

Han, J. H., Kim, M. R., Park, Y., Hong, Y. H., and Suh, H. J. (2013) Skin permeability of porcine placenta extracts and its physiological activities. Korean J. Food Sci. An. 33, 356-362. crossref(new window)

Heinrich, U., Tronnier, H., Stahl, W., Bejot, M., and Maurette, J. M. (2006) Antioxidant supplements improve parameters related to skin structure in humans. Skin Pharmacol. Physiol. 19, 224-231. crossref(new window)

Jenkins, G. (2002) Molecular mechanisms of skin ageing. Mech. Ageing Dev. 123, 801-810. crossref(new window)

Jeon, H. S., Seo, J. E., Kim, M. S., Kang, M. H., Oh, D. H., Jeon, S. O., Jeong, S. H., Choi, Y. W., and Lee, S. (2013) A retinyl palmitate-loaded solid lipid nanoparticle system: Effect of surface modification with dicetyl phosphate on skin permeation in vitro and anti-wrinkle effect in vivo. Inter. J. Pharmaceu. 452, 311-320. crossref(new window)

Jeong, H. R., Kim, J. H., Jo, Y. N., Jeong, J. H., and Heo, H. J. (2011) Characterization as cosmetic substances of chestnut inner skin extracts with antioxidant activity. J. Agric. Life Sci. 45, 183-191.

Kim, D., Pak J. I., Chae, H., Kim, Y. B., and Jang, A. R. (2013) Antioxidation effect of leg bone extracts and enzyme hydroltysates from Jeju cross bred horses (Jeju native horse * Thoroughbred). J. Life Sci. 23, 1147-1154. crossref(new window)

Kim, J. H., Cho, S. H., Seong, P. N., Hah, K. H., Jeong, J. H., Lim, D. G., Park, B. Y., Lee, J. M., Kim, D. H., and Ahn, C. N. (2007). Effect of maturity scores and number of extractions on the chemical properties of water extract from Hanwoo shank bones. Korean J. Food Sci. An. 27, 463-468. crossref(new window)

Kim, J. K., Lee, J. H., Bae, I. H., Seo, D. B., and Lee, S. J. (2011). Beneficial effect of a collagen peptide supplement on the epidermal skin barrier. Korean J. Food Sci. Technol. 43, 458-463. crossref(new window)

Masaki, H. (2010) Role of antioxidants in the skin: anti-aging effects. J. Dermatol Sci. 58, 85-90. crossref(new window)

Park, S. S., Lee, H. J., Yoon, W. J., Kang, G. J., Yang, E. J., Kim, H. S., Choo, C. S., Kang, H. K., and Yoo, E. S. (2010). Effects of horse bone extracts on the induced postmenopausal osteoporosis in rats. Kor. J. Pharmacogn. 41, 204-209.

Prior, R. L., Wu, X., and Schaich, K. (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 18; 53(10), 4290-4302. crossref(new window)

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C. (1999) Antioxidant activity applying and improved ABTS radical action decolorization assay. Free. Radical. Biol. Med. 26, 1231-123. crossref(new window)

Siebecker, A. (2005). Traditional bone broth in modern health and disease. Available from: Accessed Aug. 20, 2014.

Thring, T. S. A., Hili, P., and Naughton, D. P. (2009) Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Complementary and Alternative Medicine 9, 27. crossref(new window)

Uitto, J. and Bernstein, E. F. (1998). Molecular mechanisms of cutaneous aging: connective tissue alterations in the dermis. J. Investig. Dermatol. Symp. Proc. 3, 41-44.

Zague, V., de Freitas, V., da Costa Rosa, M., de Castro, G. A., Jaeger, R. G., and Machado-Santelli, G. M. (2011). Collagen hydrolysate intake increases skin collagen expression and suppresses matrix metalloproteinase 2 activity. J. Med. Food 14, 618-624. crossref(new window)