JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effect of Lactobacillus plantarum FH185 on the Reduction of Adipocyte Size and Gut Microbial Changes in Mice with Diet-induced Obesity
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Lactobacillus plantarum FH185 on the Reduction of Adipocyte Size and Gut Microbial Changes in Mice with Diet-induced Obesity
Park, Sun-Young; Cho, Seong-A; Lee, Myung-Ki; Lim, Sang-Dong;
  PDF(new window)
 Abstract
This study aimed to investigate the effects of Lactobacillus plantarum FH185 on the reduction of adipocyte size and gut microbial changes in mice with diet-induced obesity. The strain was found to have a lipase inhibitory activity of 70.09±2.04% and inhibited adipocyte differentiation of 3T3-L1 cells (18.63±0.98%) at a concentration of 100 µg/mL. To examine the effect of the strain supplementation on gut microbial changes in mice with diet-induced obesity, male C57BL/6J mice were fed on four different diets (i.e., A, normal diet (ND); B, high-fat diet (HFD); C, HFD with ABT-3 (109 CFU/day); and D, HFD with L. plantarum FH185 (109 CFU/day)) for 6 wk. According to the results of fecal pyrosequencing, the ratio of Firmicutes to Bacteroidetes in groups C and D was lower than in the control groups at the phylum level. At the family level, Lactobacillaceae in groups C and D was observed to dominate, while Lachnospiraceae in groups A and B was observed to dominate. At the genus level, Lactobacillus in groups C and D was comparatively higher than in groups A and B. To examine the effects of strain supplementation on the reduction of adipocyte size, the left and right epididymal fat pads were quickly isolated after the animals were sacrificed, and the adipocyte sizes were measured. In groups A, C and D, the percentage of 2,000 m2 of adipocyte was higher than in the other size of adipocyte, while the percentage of over 5,000 m2 of adipocyte was highest in group B. The mean adipocyte size of group D was significantly larger than that of group A, but smaller than that of group B.
 Keywords
Lactobacillus plantarum;anti-lipase activity;anti-adipogenetic activity;gut microbiota;adipocyte size;
 Language
English
 Cited by
1.
Probiotic Characteristics of Lactobacillus plantarum FH185 Isolated from Human Feces,;;

한국축산식품학회지, 2015. vol.35. 5, pp.615-621 crossref(new window)
1.
Probiotic Characteristics of Lactobacillus plantarum FH185 Isolated from Human Feces, Korean Journal for Food Science of Animal Resources, 2015, 35, 5, 615  crossref(new windwow)
 References
1.
An, M. A., Park, S. Y., Lee, D. K., Kim, J. R., Cha, M. K., Lee, S. W., Lim, H. T., Kim, K. J., and Nam, J. H. (2011) Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet induced obese rats. Lipids Health Dis. 10, 116-123. crossref(new window)

2.
Arora, T., Anastasovska, J., Gibson, G., Tuohy, K., Sharma, R. K., Bell, J., and Frost, G. (2012) Effect of Lactobacillus acidophilus NCDC 13 supplementation on the progression of obesity in diet-induced obese mice. Br. J. Nutr. 108, 9-1382. crossref(new window)

3.
Bhathena, J., Martoni, C. Kulamarva, A., Urbanska, A. M., Malhotra, M., and Prakash, S. (2009) Orally delivered micro encapsulated live probiotic formulation lowers serum lipids in hypercholesterolemic hamsters. J. Med. Food 12, 310-319. crossref(new window)

4.
Birari, R. B. and Bhutani, K. K. (2007) Pancreatic lipase inhibitors from natural sources: Unexplored potential. Drug. Discov. Today 12, 879-889. crossref(new window)

5.
Cani, P. D., Amar, J., Iglesias, M. A., Poggi, M., Knauf, C., Bastelica, D., Neyrinck, A. M., Fava, F., Tuohy, K. M., Chabo, C., Waget, A., Delmee, E., Cousin, B., Sulpice, T., Chamontin, B., Ferrieres, J., Tanti, J. F., Gibson, G. R., Casteilla, L., Delzenne, N. M., Alessi, M. C., and Burcelin, R. (2007) Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56, 1761-1772. crossref(new window)

6.
Cani, P. D., Bibiloni, R., Knauf, C., Neyrinck, A. M., and Delzenne, N. M. (2008) Changes in gut microbiota control metabolic diet-induced obesity and diabetes in mice. Diabetes 57, 1470-1481. crossref(new window)

7.
Delzenne, N. M., Neyrinck, A. M., Bäckhed, F., and Cani, P. D. (2011) Targeting gut microbiota in ocesity: Effects of prebiotics and probiotics. Nat. Rev. Endocrinol. 7, 639-646. crossref(new window)

8.
FAO and WHO (2001) Health and nutritional properties of probiotic in food including powder milk with live lactic acid bacteria. Report of joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Cordoba, Argentina, October 1-4, pp. 19-20.

9.
Guandalini, S. (2008) Probiotics for children with diarrhea: an update. J. Clin. Gastroenterol. 42, S53-S57. crossref(new window)

10.
Haslam D. W. and James W. P. (2005) Obesity. Lancet. 366, 1197-1209. crossref(new window)

11.
Hemati, N., Ross, S. E., Erickson, R. L, Groblewski, G. E., and MacDuygald, O. A. (1997) Signaling pathways through which insulin regulates CCAAT/enhancer binding protein-$\alpha$ (C/EBP-$\alpha$) phosphorylation and gene expression in 3T3-L1 adipocytes. Forrelation with CLUT4 gene expression. J. Biol. Chem. 272, 25913-25919. crossref(new window)

12.
Hirose, M., Ando, T., Shofiqur, R., Umeda, K., Kodama, Y., Nguyen, S. V., Goto, T., Shimada, M., and Nagaoka, S. (2013) Anti-obesity activity of hen egg anti-lipase immunoglobulin yolk, a novel pancreatic lipase inhibitor. Nutr. Metab. 10, 70-76. crossref(new window)

13.
Hirsch, J. and Gallian, E. (1968) Methods for the determination of adipose cell size in man and animals. J. Lipid Res. 9, 110-119.

14.
Isolauri, E. and Salminen, S. (2008) Probiotics: Use in allergic disorders: A Nutrition, Allergy, Mucosal Immunology, and Intestinal Microbiota (NAMI) research group report. J. Clin. Gastroenterol. 42, S91-S96. crossref(new window)

15.
Iwashita, S., Tanida, M., Terui, N., Ootsuka, Y., Shu, M., Kang, D., and Suzuki, M. (2002) Direct measurement of renal sympathetic nervous activity in high-fat diet-related hypertensive rats. Life Sci. 71, 46-537.

16.
Kumar, M., Kumar, A., Nagpal, R., Mohania, E., Behare, P., Verma, V., Kumar, P., Poddar, D., Aggarwal, P. K., Henry, C. J., Jain, S., and Yadav, H. (2010) Cancer-preventing attributes of probiotics: an update. Int. J. Food Sci. Nutr. 61, 473-496. crossref(new window)

17.
Lay, C., Sutren, M., Rochet, V., Saunier, K., Doré, J., and Rigottier-Gois, L. (2005) Design and validation of 16S rDNA probes to enumerate members of the Clostridium leptum subgroup in human faecal microbiota. Environ. Microbiol. 7, 933-946. crossref(new window)

18.
Lee, K., Paek, K., Lee, H. Y., Park, J. H., and Lee, Y. (2007) Anti obesity effect of trans-10, cis-12 conjugated linoleic acidproducing Lactobacillus plantarum PL62 on diet-induced obese mice. J. Appl. Microbiol. 103, 1140-1146. crossref(new window)

19.
Lee, Y. P., Chung G. H., and Rhee, J. S. (1993) Purification and characterization of Pseudomonas fluorescens SIK W1 lipase expressed in Escherichia coli. Biochim. Biophys. Acta. 1169, 156-164. crossref(new window)

20.
Ley, R. E., Turnbaugh, P., Klein, S., and Gordon, J. I. (2006) Microbial ecology human gut microbes associated with obesity. Nature 444, 1022-1023. crossref(new window)

21.
Lim, S. D., Kim, K. S., and Do, J. R. (2011) Physiological characteristics and production of vitamin K2 by Lactobacillus fermentum LC272 isolated from raw milk. Korean J. Food Sci. Ani. Resour. 31, 513-520. crossref(new window)

22.
Liu, W. T., Marsh, T. L., Cheng, H., and Forney, L. J. (1997) Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl. Environ. Microbiol. 63, 4516-4522.

23.
Mennigen, R. and Bruewer, M. (2009) Effect of probiotics on intestinal barrier function. Ann. NY. Acad. Sci. 1165, 183-189. crossref(new window)

24.
Nava, G. M., Friedrichsen, H. J., and Stappenbeck, T. S. (2011) Spatial organization of intestinal microbiota in the mouse ascending colon. ISME J. 5, 627-638. crossref(new window)

25.
Nguyen, T. D., Kang, J. H., and Lee, M. S. (2007) Characterization of Lactobacillus plantarum PH04, a potential probiotic bacterium with cholesterol-lowering effects. Int. J. Food Microbiol. 113, 358-361. crossref(new window)

26.
Perdigón, G., Fuller, R., and Raya, R. (2001) Lactic acid bacteria and their effect on the immune system. Curr. Issues Intest. Microbiol. 2, 27-42.

27.
Ramirez-Zacarias, J. L., Castro-Munozledo, F., and Kuri-Harcuch, W. (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochem. 97, 493-497. crossref(new window)

28.
Rogosa, M. (1974) Genus I. Lactobacillus. In: Bergey’s manual of determinative bacteriology. 8th ed. Buchanan, R. E. and Gibbons, N. E. (eds), Williams and Wilkins Co., Baltimore, pp. 576-593.

29.
Schloss, P. D. and Handelsman, J. (2008) A statistical toolbox for metagenomics: assessing functional diversity in microbial communities. BMC Bioinformatics 9, 34. crossref(new window)

30.
Stein, C. J. and Colditz, G. A. (2004) The epidemic of obesity. J. Clin. Endocrinol. Metab. 89, 2522-2525. crossref(new window)

31.
Tanida, M., Shen, J., Maeda, K., Horii, Y., Yamano, T., Fukushima, Y., and Nagai, K. (2008) High-fat diet-induced obesity is attenuated by probiotic strain Lactobacillus paracasei ST11 (NCC2461) in rats. Obes. Res. Clin. Pract. 2, 1-11. crossref(new window)

32.
Turnbaugh, P. J., Hamady, M., Yatsunenko, T., Cantarel, B. L., Duncan, A., Ley, R. E., Sogin, M. L., Jones, W. J., Roe, B. A., Affourtit, J. P., Egholm, M., Henrissat, B., Heath, A. C., Knight, R., and Gordon, J. I. (2009) A core gut microbiome in obese and lean twins. Nature 457, 480-484. crossref(new window)

33.
van Goor, H., Gerrits, P. O., and Grond, J. (1986) The application of lipid-soluble stains in plastic-embedded sections. Histochemistry 85, 251-253. crossref(new window)