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The Synergic Anti-inflammatory Impact of Gleditsia sinensis Lam. and Lactobacillus brevis KY21 on Intestinal Epithelial Cells in a DSS-induced Colitis Model
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 Title & Authors
The Synergic Anti-inflammatory Impact of Gleditsia sinensis Lam. and Lactobacillus brevis KY21 on Intestinal Epithelial Cells in a DSS-induced Colitis Model
Kim, Younghoon; Koh, Ji Hoon; Ahn, Young Jun; Oh, Sejong; Kim, Sea Hun;
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We investigated the synergic anti-inflammatory activity of Gleditsia sinensis Lam. (GS) extract and Lactobacillus brevis KY21 both in vitro and in vivo. Western blot analysis and immunostaining showed that AKT phosphorylation that increased by the exposure of LPS were significantly decreased by the presence of either GS extract or L. brevis KY21. In addition, p65 intracellular transport was critically inhibited by GS extract and L. brevis KY21. We further studied these effects using an in vivo dextran sulfate sodium (DSS)-induced mouse model. Body weight, food intake, and clinical scores were dramatically decreased after treatment with DSS, whereas these effects were palliated by the addition of GS extract and L. brevis KY21. Importantly, transcription of genes encoding pro-inflammatory cytokines including IL-1β, TNF-α, and IFN-γ in mesenteric lymph nodes (MLN) and the spleen were increased by DSS treatment, whereas they were inhibited by the presence of GS extract and L. brevis KY21.
anti-inflammatory activity;herbal extract;probiotic bacteria;DSS;
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Abreu-Martin, M. T., Vidrich, A., Lynch, D. H., and Targan, S. R. (1995) Divergent induction of apoptosis and IL-8 secretion in HT-29 cells in response to TNF-alpha and ligation of Fas antigen. J. Immunol. 155, 4147-4154.

Baggiolini, M., Walz, A., and Kunkel, S. L. (1989) Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J. Clin. Invest. 84, 1045-1049. crossref(new window)

Bamias, G., Corridoni, D., Pizarro, T. T., and Cominelli, F. (2012) New insights into the dichotomous role of innate cytokines in gut homeostasis and inflammation. Cytokine 59, 451-459. crossref(new window)

Bedaiwi, M. K. and Inman, R. D. (2014) Microbiome and probiotics: Link to arthritis. Curr. Opin. Rheumatol. 26, 410-415. crossref(new window)

Chow, L. M., Chui, C. H., Tang, J. C., Teo, I. T., Lau, F. Y., Cheng, G. Y., Wong, R. S., Leung, T. W., Lai, K. B., Yau, M. Y., Gou, D., and Chan, A. S. (2003) Gleditsia sinensis fruit extract is a potential chemotherapeutic agent in chronic and acute myelogenous leukemia. Oncol. Rep. 10, 1601-1607.

Christensen, H., Bisgaard, M., Angen, O., and Olsen, J. E. (2002) Final classification of Bisgaard taxon 9 as Actinobacillus arthritidis sp. nov. and recognition of a novel genomo-species for equine strains of Actinobacillus lignieresii. Int. J. Syst. Evol. Microbiol. 52, 1239-1246.

Dieleman, L. A., Palmen, M. J., Akol, H., Bloemena, E., Peña, A. S., Meuwissen, S. G., and Van Rees, E. P. (1998) Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines. Clin. Exp. Immunol. 114, 385-391. crossref(new window)

FAO/WHO (2002) Joint FAO/WHO Working Group Report on Drafting Guidelines for the evaluation of Probiotics in Food.

Fujimori, S., Tatsuguchi, A., and Gudis, K. (2007) High dose probiotic and prebiotic cotherapy for remission induction of active Crohn's disease. J. Gastroenterol. Hepatol. 22, 1199-1204. crossref(new window)

Howarth, G. S., Xian, C. J., and Read, L. C. (1998) Insulin-like growth factor-I partially attenuates colonic damage in rats with experimental colitis induced by oral dextran sulphate sodium. Scand. J. Gastroenterol. 33, 180-190. crossref(new window)

Jamaluddin, M., Casola, A., Garofalo, R. P., Han, Y., Elliott, T., Ogra, P. L., and Brasier, A. R. (1998) The major component of IκBα proteolysis occurs independently of the proteasome pathway in respiratory syncytial virus-infected pulmonary epithelial cells. J. Virol. 72, 4849-4857.

Lee, Y. S., Han, O. K., Park, C. W., Yang, C. H., Jeon, T. W., Yoo, W. K., Kim, S. H., and Kim, H. J. (2005) Pro-inflammatory cytokine gene expression and nitric oxide regulation of aqueous extracted Astragali radix in RAW 264.7 macrophage cells. J. Ethnopharmacol. 100, 289-294. crossref(new window)

Li, W. H., Zhang, X. M., Tian, R. R., Zheng, Y. T., Zhao, W. M., and Qiu, M. H. (2007) A new anti-HIV lupane acid from Gleditsia sinensis Lam. J. Asian Nat. Prod. Res. 9, 551-555. crossref(new window)

Madsen, K. A. C., Soper, P., McKaigney, C. H. J., Yachimec, C., Doyle, J., Jewell, L., and De Simone, C. (2001) Probiotic bacteria enhance murine and human intestinal epithelial barrier function. Gastroenterol. 121, 580-591. crossref(new window)

Mantovani, A., Bussolino, F., and Dejana, E. (1992) Cytokine regulation of endothelial cell function. FASEB J. 6, 2591-2599. crossref(new window)

Mizoguchi, A. and Mizoguchi, E. (2008) Inflammatory bowel disease, past, present and future: Lessons from animal models. J. Gastroenterol. 43, 1-17. crossref(new window)

Okayasu, I., Hatakeyama, S., Yamada, M., Ohkusa, T., Inagaki, Y., and Nakaya, R. (1990) A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterol. 98, 694-702. crossref(new window)

Osawa, Y., Nagaki, M., Banno, Y., Brenner, D. A., Asano, T., Nozawa, Y., Moriwaki, H., and Nakashima, S. (2002) Tumor necrosis factor alpha-induced interleukin-8 production via NF-kappaB and phosphatidylinositol 3-kinase/Akt pathways inhibits cell apoptosis in human hepatocytes. Infect. Immun. 70, 6294-6301. crossref(new window)

Podolsky, D. K. (2005) Inflammatory bowel disease. N. Engl. J. Med. 358, 1965-1968.

Rumi, G., Tsubouchi, R., Okayama, M., Kato, S., Mózsik, G., and Takeuchi, K. (2004) Protective effect of lactulose on dextran sulfate sodium-induced colonic inflammation in rats. Dig. Dis. Sci. 49, 1466-1472. crossref(new window)

Takahashi, A., Wada, A., Ogushi, K., Maeda, K., Kawahara, T., Mawatari, K., Kurazono, H., Moss, J., Hirayama, T., and Nakaya, Y. (2001) Production of beta-defensin-2 by human colonic epithelial cells induced by Salmonella enteritidis flagella filament structural protein. FEBS Lett. 508, 484-488. crossref(new window)

Weber, C. K., Liptay, S., Wirth, T., Adler, G., and Schmid, R. M. (2000) Suppression of NF-kB activity by sulfasalazine is mediated by direct inhibition of IkB kinases alpha and beta. Gastroenterol. 119, 1209-1218. crossref(new window)

Yi, J. M., Kim, J., Park, J. S., Lee, J., Lee, Y. J., Hong, J. T., Bang, O. S., and Kim, N. S. (2015) In vivo anti-tumor effects of the ethanol extract of Gleditsia sinensis thorns and its active constituent, cytochalasin H. Biol. Pharm. Bull. 38, 909-912. crossref(new window)