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Whitening Effect of Watersoluble Royal Jelly from South Korea
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 Title & Authors
Whitening Effect of Watersoluble Royal Jelly from South Korea
Han, Sang Mi; Kim, Jung Min; Hong, In Phyo; Woo, Soon Ok; Kim, Se Gun; Jang, Hye Ri; Park, Kwan Kyu; Pak, Sok Cheon;
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 Abstract
Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein. In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F1 melanoma cells were first treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of WSRJ. In addition, we investigated the mRNA and protein expression of melanogenesis-related genes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2 by reverse transcription-polymerase chain reaction and western blotting. WSRJ directly inhibited tyrosinase and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells a level comparable to that observed with arbutin. WSRJ decreased the mRNA and protein expressions of tyrosinase, TRP-1, and TRP-2, which was comparable to that observed with arbutin. WSRJ has strong anti-melanogenic activity, which invoice direct inhibition of tyrosinase enzyme activity and suppression of expression of melanogenesis related genes. Results from this study suggests that WSRJ is a potential candidate for the treatment of skin pigmentation.
 Keywords
royal jelly;water-soluble royal jelly;α-MSH;tyrosinase;TRP-1;TRP-2;
 Language
English
 Cited by
1.
Occupational allergic respiratory disease due to royal jelly, Annals of Allergy, Asthma & Immunology, 2016, 117, 1, 102  crossref(new windwow)
 References
1.
Ando, H., Funasaka, Y., Oka, M., Ohashi, A., Furumura, M., Matsunaga, J., Matsunaga, N., Hearing, V. J., and Ichihashi, M. (1999) Possible involvement of proteolytic degradation of tyrosinase in the regulatory effect of fatty acids on melanogenesis. J. Lipid Res. 40, 1312-1316.

2.
Barsh, G. S. (1996) The genetics of pigmentation: From fancy genes to complex traits. Trends Genet. 12, 299-305. crossref(new window)

3.
Buscà, R. and Ballotti, R. (2000) Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res. 13, 60-69. crossref(new window)

4.
Chang, T. S. and Chen, C. T. (2012) Inhibitory effect of homochlorcyclizine on melanogenesis in α-melanocyte stimulating hormone-stimulated mouse B16 melanoma cells. Arch. Pharm. Res. 35, 119-127. crossref(new window)

5.
Food and Agriculture Organization of the United Nations (FAO) (2007) Value-added products from beekeeping. http://www.fao.org/docrep/w0076E/w0076E00.htm.

6.
Han, S. M., Yeo, J. H., Cho, Y. H., and Pak, S. C. (2011) Royal jelly reduces melanin synthesis through down-regulation of tyrosinase expression. Am. J. Chin. Med. 39, 1253-1260. crossref(new window)

7.
Hearing, V. J. (1999) Biochemical control of melanogenesis and melanosomal organization. J. Investig. Dermatol. Symp. Proc. 4, 24-28. crossref(new window)

8.
Hearing, V. J. and Tsukamoto, K. (1991) Enzymatic control of pigmentation in mammals. FASEP J. 5, 2902-2909.

9.
Imokawa, G. (2004) Autocrine and paracrine regulation of melanocytes in human skin and in pigmentary disorders. Pigment Cell Res. 17, 96-110. crossref(new window)

10.
Jozef, S. (2001) Some properties of the main protein of honeybee (Apis mellifera) royal jelly. Apidologie. 32, 69-80. crossref(new window)

11.
Kim, J. M., Han, S. M., Cho, M. R., Lee, K. G., Lee, M. L., Lee, M. Y., Woo, S. O., Hong, I. P., Sim, H. S., and Choi, Y. S. (2013) Characterization of water soluble royal jelly removed allergenic protein. Korean J. Apiculture 28, 19-23.

12.
Kohno, K., Okamoto, I., Sano, O., Arai, N., Iwaki, K., Ikeda, M., and Kurimoto, M. (2004) Royal jelly inhibits the production of proinflammatory cytokines by activated macrophages. Biosci. Biotechnol. Biochem. 68, 138-145. crossref(new window)

13.
Koya-Miyata, S., Okamoto, I., Ushio, S., Iwaki, K., Ikeda, M., and Kurimoto, M. (2004) Identification of a collagen production-promoting factor from an extract of royal jelly and its possible mechanism. Biosci. Biotechnol. Biochem. 68, 767-773. crossref(new window)

14.
Lee, E. H,, Lim, Y. J., Ha, S. K., Kang, T. H., Koketsu, M., Kang, C., Kim, S. Y., and Park, J. H. (2010) Inhibitory effects of 5-chloroacetyl-2-piperidino-1,3-selenazole, a novel selenium-containing compound, on skin melanin biosynthesis. J. Pharm. Pharmacol. 62, 352-359. crossref(new window)

15.
Lercker, G., Capella, P., Conte, L. S., Ruini, F., and Giordani, G. (1982) Components of royal jelly II. The lipid fraction, hydrocarbons and sterols. J. Apicultural Res. 21, 178-184.

16.
Park, H. M., Cho, M. H., Cho, Y., and Kim, S. Y. (2012) Royal jelly increases collagen production in rat skin after ovariectomy. J. Med. Food 15, 568-575. crossref(new window)

17.
Park, H. M., Hwang, E. S., Lee, K. G., Han, S. M., Cho, Y. H., and Kim, S. Y. (2011) Royal jelly protects against ultraviolet B-induced photoaging in human skin fibroblast via enhancing collagen production. J. Med. Food 14, 899-906. crossref(new window)

18.
Rosmilah, M., Shahnaz, M., Patel, G., Lock, J., Rahman, D., Masita, A., and Noormalin, A. (2008) Characterization of major allergens of royal jelly Apis mellifera. Trop. Biomed. 25, 243-251.

19.
Solano, F., Briganti, S., Picardo, M., and Ghanem, G. (2006) Hypopigmenting agents: an updated review on biological,chemical and clinical aspects. Pigment Cell Res. 19, 550-571. crossref(new window)

20.
Tolanai, S. and Morgan, J. F. (1962) Studies on the in vitro antitumor activity of fatty acids. V. Unsaturated acids. Can. J. Biochem. Physiol. 40, 869-875. crossref(new window)

21.
Tokunaga, K. H., Yoshida, C., Suzuki, K. M., Maruyama, H., Futamura, Y., Araki, Y., and Mishima, S. (2004) Antihypertensive effect of peptides from royal jelly in spontaneously hypertensive rats. Biol. Pharm. Bull. 27, 189-192. crossref(new window)

22.
Urabe, K., Nakayama, J., and Hori, Y. (1998) The pigmentary system: physiology and pathophysiology. Oxford University Press, New York, pp. 760-766.

23.
Yokota, T., Nishio, H., Kubota, Y., and Mizoguchi, M. (1998) The inhibitory effect of glabridin from licorice extracts on melanogenesis and inflammation. Pigment Cell Res. 11, 355-361. crossref(new window)