Advanced SearchSearch Tips
Analytical Determination of Vitamin B12 Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Analytical Determination of Vitamin B12 Content in Infant and Toddler Milk Formulas by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Lee, Jung-Hoon; Shin, Jin-Ho; Park, Jung-Min; Kim, Ha-Jung; Ahn, Jang-Hyuk; Kwak, Byung-Man; Kim, Jin-Man;
  PDF(new window)
The development of a sample preparation method and optimization of the analytical instrumentation conditions were performed for the determination of the vitamin B12 content in emulsified baby foods sold on the Korea market. After removal of the milk protein and fats by chloroform extraction and centrifugation, the vitamin B12 was water extracted from the sample. Following filtration of the solution through a nylon filter, the water-soluble extract was purified by solid-phase extraction using a Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). The solution eluted from the cartridge was dried under a stream of nitrogen gas and reconstituted with 1 mL of water. The sample solution was injected into an LC-MS/MS system after optimizing the mobile phase for vitamin B12 detection. The calibration curve showed good linearity with the coefficient of correlation (r2) value of 0.9999. The limit of detection was 0.03 µg/L and the limit of quantitation was 0.1 µg/L. The method of detection limit was 0.02 µg/kg. The vitamin B12 recovery from a spiking test was 99.62% for infant formula and 99.46% for cereal-based baby food. The sample preparation method developed in this study would be appropriate for the rapid determination of the vitamin B12 content in infant formula and baby foods with emulsified milk characteristics. The ability to obtain stable results more quickly and efficiently would also allow governments to exercise a more extensive quality control inspection and monitoring of products expected to contain vitamin B12. This method could be implemented in laboratories that require time and labor saving.
Vitamin B12;Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS);analytical method;infant formula;toddler formula;
 Cited by
Influence of food groups on plasma total homocysteine for specificMTHFR C677Tgenotypes in Chinese population, Molecular Nutrition & Food Research, 2017, 61, 2, 1600351  crossref(new windwow)
Ahn, H. S. and Um, S. S. (2003) Dietary intakes of infants and young children in Seoul area. J. Korean Soc. Matern. Child Health 7, 179-191. crossref(new window)

AOAC International (2002) AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals. Association of Official Analytical Chemists, Gaithersburg, MD. 1-38.

Baiyi, L. B., Ren, Y., Huang, B., Liao, W., Cai, Z., and Tie, X. (2008) Simultaneous determination of four water-soluble vitamins in fortified infant foods by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry. J. Chromatogr. 46, 225-232.

Council for Responsible Nutrition (2014) Vitamin and Mineral Safety. 3rd. 94-97.

Dror, D. and Allen, L. (2008) Effect of vitamin B12 deficiency on neurodevelopment in infants. Nutr. Rev. 66, 250-255. crossref(new window)

Food and Nutrition Board (1998) Institute of Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline, Washington D.C. 306-356.

Ministry of Food and Drug Safety. (2012) Food Code General Test Methods Vitamin B12.

Friedrich, W. (1988) Vitamins: Vitamin B12. Walter de Gruyter. 837-928.

Guerra-Shinohara, E. M., Paiva, A. A., Rondo, Yamasakia, K., and Terzic, C. A. (2002) Relationship between total homocysteine and folate levels in pregnant women and their newborn babies according to maternal serum levels of vitamin B12. BJOG. 109, 784-791. crossref(new window)

Herbert, V. (1987) Recommended dietary intakes of vitamin B12 in humans. Am. J. Clin. Nutr. 45, 671-678.

Kirchner, U., Degenhardt, K., Raffler, G., and Nelson, M. (2011) Determination of vitamin B12 in infant formula and adult nutritionals using HPLC after purification on an immunoaffinity column: First action 2011.09. J. AOAC Int. 95, 933-936.

KS A ISO 5725 (2002) Accuracy(trueness and precision) of Measurement methods and results.

MFDS (Ministry of Food and Drug Safety). (2012) Medicines such as guidelines for the application of the test method validation handbook (Revised). Pharmaceuticals Evaluation Department. 1-45.

Min, H. S. and Kim, M. S. (2009) A critical evaluation of the correlation between biomarkers of folate and vitamin B12 in nutritional homocysteinemia. Korean J. Nutr. 42, 423-433. crossref(new window)

Moon, C. J. (2007) Used in health food vitamin / mineral for evaluation guide. MFDS. 97-107.

Om, A. S., Lee, H. O., Moon. J. H., Shim, J. Y., Kim, I. H., Won, S. I., Rha. Y. A., Choi, Y. J., Lee, H. Y., Park, H. K., and Kim, M. C. (2007) A study on the amendment scheme of nutrient standard regulations for infant formula in Korea. J. Korean Soc. Food Sci. Nutr. 36, 569-577. crossref(new window)

The Korean Nutrition Society (2009) Food and nutrients sourcebook. Korea editorial Nutrition. 78-88.

Youn, H. S. (2005) New nutritional concepts of vitamins and minerals. Korean J. Pediatr. 48, 1295-1309.