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Inactivation of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus brevis in Low-fat Milk by Pulsed Electric Field Treatment: A Pilot-scale Study
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 Title & Authors
Inactivation of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus brevis in Low-fat Milk by Pulsed Electric Field Treatment: A Pilot-scale Study
Lee, Gun Joon; Han, Bok Kung; Choi, Hyuk Joon; Kang, Shin Ho; Baick, Seung Chun; Lee, Dong-Un;
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We investigated the effects of a pulsed electric field (PEF) treatment on microbial inactivation and the physical properties of low-fat milk. Milk inoculated with Escherichia coli, Saccharomyces cerevisiae, or Lactobacillus brevis was supplied to a pilot-scale PEF treatment system at a flow rate of 30 L/h. Pulses with an electric field strength of 10 kV/cm and a pulse width of 30 µs were applied to the milk with total pulse energies of 50-250 kJ/L achieved by varying the pulse frequency. The inactivation curves of the test microorganisms were biphasic with an initial lag phase (or shoulder) followed by a phase of rapid inactivation. PEF treatments with a total pulse energy of 200 kJ/L resulted in a 4.5-log reduction in E. coli, a 4.4-log reduction in L. brevis, and a 6.0-log reduction in S. cerevisiae. Total pulse energies of 200 and 250 kJ/L resulted in greater than 5-log reductions in microbial counts in stored PEF-treated milk, and the growth of surviving microorganisms was slow during storage for 15 d at 4℃. PEF treatment did not change milk physical properties such as pH, color, or particle-size distribution (p<0.05). These results indicate that a relatively low electric-field strength of 10 kV/cm can be used to pasteurize low-fat milk.
pulsed electric field;low-fat milk;microbial inactivation;pasteurization;physical property;
 Cited by
Agcam, E., Akyýldýz, A., and Evrendilek, G. (2014) Comparison of phenolic compounds of orange juice processed by pulsed electric fields (PEF) and conventional thermal pasteurisation. Food Chem. 143, 354-361. crossref(new window)

Bendicho, S., Espachs, A., Stevens, D., Arantegui, J., and Martýn, O. (1999) Effect of high intensity pulsed electric fields on vitamins of milk. European Conference on Emerging Food Science and Technology, pp. 108.

Buckow, R., Ng, S., and Toepfl, S. (2013) Pulsed electric field processing of orange juice: a review on microbial, enzymatic, nutritional, and sensory quality and stability. Comp. Rev. Food Sci. Food Saf. 12, 455-467. crossref(new window)

Calderon-Miranda, M. L., Barbosa-Canovas, G. V., and Swanson, B. G. (1999) Inactivation of Listeria innocua in skim milk by pulsed electric fields and nisin. Int. J. Food Microbiol. 51, 19-30. crossref(new window)

Dunn, J. E. and Pearlman, J. S. (1987) Methods and apparatus for extending the shelf life of fluid food products. U.S. Patent 4,695,472.

Evrendilek, G. A., Zhang, Q. H., and Richter, E. R. (2005) Application of pulsed electric fields to skim milk inoculated with Staphylococcus aureus. Biosyst. Eng. 87, 137-144.

Fernandez-Molina, J. J., Barbosa- Cánovas, G. V., and Swanson, B. G. (2005) The combined effect of pulsed electric fields and conventional heat on the microbial quality and shelf life of skim milk. J. Food Process Preserv. 29, 390-406. crossref(new window)

Food and Drug Administration. (2011) Grade “A” pasteurized milk ordinance. Available at (Accessed Jan. 30, 2015).

Guerrero-Beltrán, J. Á., Sepulveda, D. R., Góngora-Nieto, M. M., Swanson, B., and Barbosa-Cánovas, G. V. (2010) Milk thermization by pulsed electric fields (PEF) and electrically induced heat. J. Food Eng. 100, 56-60. crossref(new window)

In, Y. M. and Jung, J. K. (2001) Review on the change of physicochemical quality during heating of milk. Kor. J. Dairy Sci. Technol. 19, 13-21.

Jaeger, H., Schulz, A., Karapetkov, N., and Knorr, D. (2009) Protective effect of milk constituents and sublethal injuries limiting process effectiveness during PEF inactivation of Lb. rhamnosus. Int. J. Food Microbiol. 134, 154-161. crossref(new window)

Knorr, D., Angersbach, A., Eshtiaghi, M. N., Heinz, V., and Lee, D. U. (2001) Processing concepts based on high intensity electric field pulses. Trends Food Sci. Tech. 12, 129-135. crossref(new window)

Li, S. Q., Zhang, Q. H., Pham, V. T. V., and Lee, Y. Z. (2002) Inactivation of natural flora and E. coli 8739 in a milk protein concentrate-fortified soymilk. IFT meeting.

Michalac, S. L., Alvarez, V. B., and Zhang, Q. H. (1999) Microbial reduction in skim milk using PEF technology. IFT meeting.

Qin, B. L., Barbosa-Cánovas, G. V., Swanson, B. G., and Pedrow, P. D. (1998) Inactivating microorganisms using a pulsed electric field continuous treatment system. IEEE Trans. Ind. Appl. 34, 43-50.

Qin, B. L., Vega-Mercado, H., Potakamury, U. R., and Barbosa-Cánovas, G. V. (1995) Application of pulsed electric fields for the inactivation of bacteria and enzymes. J. Franklin Inst. 332, 209-220.

Rivas, A., Rodrigo, D., Martínez, A., Barbosa-Cánovas, G. V., and Rodrigo, M. (2006) Effect of PEF and heat pasteurization on the physical-chemical characteristics of blended orange and carrot juice. LWT-Food Sci. Technol. 39, 1163-1170. crossref(new window)

Rowan, N. J., MacGregor, S. J., Anderson, J. G., Cameron, D., and Farish, O. (2001) Inactivation of Mycobacterium paratuberculosis by pulsed electric fields. Appl. Environ. Microbiol. 67, 2833-2836. crossref(new window)

Sampedro, F., Rodrigo, M., Martinez, A., Rodrigo, D., and Barbosa-Canovas, G. V. (2005) Quality and safety aspects of PEF application in milk and milk products. Crit. Rev. Food Sci. Nutr. 45, 25-47. crossref(new window)

Sepulveda, D. R., Gongora-Nieto, M. M., Guerrero, J. A., and Barbosa-Canovas, G. V. (2005) Production of extended-shelf life milk by processing pasteurized milk with pulsed electric fields. J. Food Eng. 67, 81-86. crossref(new window)

Yoo, S. H., Kang, S. B., Park, J. H., Lee, K. S., Kim, J. M., and Yoon, S. S. (2013) Effect of heat-treat methods on the soluble calcium levels in the commercial milk products. Korean J. Food Sci. An. 33, 369-376. crossref(new window)

Zhang, Q., Barbosa-Cánovas, G. V., and Swanson, B. G. (1995) Engineering aspects of pulsed electric field pasteurization. J. Food Eng. 25, 261-281. crossref(new window)

Zimmermann, U. (1986) Electrical breakdown, electropermeabilization and electrofusion. Rev. Physiol. Biochem. Pharmacol. 105, 175-256.