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Antidiabetic Potential of Kefir Combination from Goat Milk and Soy Milk in Rats Induced with Streptozotocin-Nicotinamide
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 Title & Authors
Antidiabetic Potential of Kefir Combination from Goat Milk and Soy Milk in Rats Induced with Streptozotocin-Nicotinamide
Nurliyani,; Harmayani, Eni; Sunarti,;
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The study aimed to evaluate the effect of kefir combination from goat milk and soy milk on lipid profile, plasma glucose, glutathione peroxidase (GPx) activity and the improvement of pancreatic β-cell in diabetic rats. Male rats were divided into five treatments: normal control, diabetic control, goat milk kefir, combination of goat milk-soy milk kefir and soy milk kefir. All rats were induced by streptooztocin-nicotinamide (STZ-NA), except for normal control. After 35 d experiment, the rats were sampled for blood, sacrificed and sampled for pancreatic tissues. Results showed that diabetic rats fed kefir combination had higher (p<0.05) triglyceride than the rats fed goat milk or soy milk kefir. Decreasing of plasma glucose in diabetic rats fed kefir combination was higher (p<0.05) than rats fed goat millk kefir. The activity of GPx in diabetic rats fed three kinds of kefir were higher (p<0.01) than untreated diabetic rats. The average number of Langerhans and β-cells in diabetic rats fed kefir combination was the same as the normal control, but it was higher than diabetic control. It was concluded that kefir combination can be used as antidiabetic through maintaining in serum triglyceride, decreasing in plasma glucose, increasing in GPx activity and improving in pancreatic β-cells.
kefir combination;lipid profile;plasma glucose;glutathione peroxidase;β-cells;
 Cited by
ADA (American Diabetes Association) (2004) Diagnosis and classification of diabetes mellitus. Diabetes Care 27, S5-S10. crossref(new window)

ADA (American Diabetes Association) (2008) Diagnosis and classification of diabetes mellitus. Diabetes Care 31, S55-S60. crossref(new window)

Alsayadi, M., Al- Jawfi, Y., Belarbi, M., Soualem-Mami, Z., Merzouk, H., Sari, D. C., Sabri, F., and Ghalim, M. (2014) Evaluation of anti-hyperglycemic and anti-hyperlipidemic activities of water kefir as probiotic on streptozotocin-induced diabetic wistar rats. J. Diabetes Mellitus 4, 85-95. crossref(new window)

Bertoni, A. G., Hundley, W. G., Massing, M. W., Bonds, D. E., Burke, G. L., and Goff, D. C. Jr. (2004) Heart failure prevalence, incidence, and mortality in the elderly with diabetes. Diabetes Care 27, 699-703. crossref(new window)

Bolanho, B. C. and Beléia, A. D. P. (2011) Bioactive compounds and antioxidant potential of soy products. Alim. Nutr. 22, 539-546.

Cerf, M. E. (2013) Beta cell dysfunction and insulin resistance. Front. Endocrinol. 4, 1-12.

Domaniç, N., Gelisgen, R., Civelek, S., Demir, A. S., Ural, D., Andican, G. G., Vural, V. A., and Burçak, G. (2006) Homocysteine and nitric oxide in patients undergoing diagnostic coronary angiography. Acta Med. Okayama 60, 35-41.

Ebner, J., Arslan, A. A., Fedorova, M., Hoffmann, R., Küçükçetin, A., and Pischetsrieder, M. (2015) Peptide profiling of bovine kefir reveals 236 unique peptides released from caseins during its production by starter culture or kefir grains. J. Proteomics 18, 41-57.

Ghasemi, A., Khalifi, S., and Jedi, S. (2014) Streptozotocin-nicotinamide-induced rat model of type 2 diabetes. Acta Physiol. Hung. 101, 408-420. crossref(new window)

Hadisaputro, S., Djokomoeljanto, R. R., Judiono, and Soesatyo, M. H. (2012) The effects of oral plain kefir supplementation on proinflammatory cytokine properties of the hyperglycemia Wistar rats induced by streptozotocin. Acta Med. Indones. 44, 100-104.

Haenlein, G. F. W. (2004) Goat milk in human nutrition. Small Ruminant Res. 51, 155-163. crossref(new window)

Heneman, K., Steinberg, F., and Zidenberg-Cherr, S. (2007) Some fact about soy. Nutrition and Health Info-Sheet. UC Cooperative Extension Center for Health and Nutrition Research, Department of Nutrition, University of California. 1-4.

Huang, H.Y., Korivi, M., Tsai, C. H., Yang, J. H., and Ying-Chieh, T. (2013) Supplementation of lactobacillus plantarum k68 and fruit-vegetable ferment along with high fat-fructose diet attenuates metabolic syndrome in rats with insulin resistance. Evid. Based Complement. Alternat. Med. 1-12.

Isfahlan, A. J., Mahmoodzadeh, A., Hassanzadeh, A., Heidari, R., and Jamei, R. (2010) Antioxidant and antiradical activities of phenolic extracts from Iranian almond (Prunus amygdalus L.) hulls and shells. Turk. J. Biol. 34, 165-173.

Jascolka, T. L., Aguilar, E.C., Teixeira, L. G., Lages, P. C., de Cássia Raimundo, I., Beltrão, N. R. M., de Oliveira Matoso, R., Carneiro, R. P., Nicoli, J. R., and Alvarez-Leite, J. I. (2013) Kefir Supplementation Improves lipid profile and oxidative stress but does not reduce atherosclerotic lesion in apoE deficient mice. J. Food Nutr. Disor. 2, 1-7.

Jeppesen, J., Hein, H. O., Suadicani, P., and Gyntelberg, F. (1997) Relation of high TG-low HDL cholesterol and LDL cholesterol to the incidence of ischemic heart disease. An 8-year follow-up in the Copenhagen Male Study. Arterioscler. Thromb. Vasc. Biol. 17, 1114-1120. crossref(new window)

Jones, S. E., Paynich, M. L., and Knight, K. L. (2014) Exopolysaccharides: Sweet success with probiotic therapeutics. Inflamm. Cell Signal. 1, 2-8.

Kanter, M., Coskun, O., Korkmaz, A., and Oter, S. (2014) Effects of Nigella sativa on oxidative stress and -cell damage in streptozotocin-induceddiabeticrats. Anat. Rec. A 279A, 685-691.

Kesenkas, H., Dinkci, N., Seckin, K., Kinik, O., and Gonc, S. (2011) Antioxidant properties of kefir produced from different cow and soy milk mixtures. J. Agric. Sci. 17, 253-259.

Kikui, Y., Seguchi, H., and Mizoguti, H. (1977) A differential staining method for A- and B- cells in the pancreatic islets of Langerhans. Acta Histochem. Cytochem. 10, 10-13. crossref(new window)

Kýlýc, S., Uysal, H., Akbulut, N., Kavas, G., and Kesenkas, H. (1999) Chemical, microbiological and sensory changes in ripening kefirs produced from starters and grains. Ege University Journal of Agricultural Faculty 36, 111-118.

Kurowska, E. M. and Carroll, K. K. (1992) Effect of high levels of selected dietary essential amino acids on hypercholesterolemia and down-regulation of hepatic LDL receptors in rabbits. Biochim. Biophys. Acta 1126, 185-191. crossref(new window)

Kurowska, E. M. and Carroll, K. K. (1994) Hypercholesterolemic responses in rabbits to selected groups of dietary essential amino acids. J. Nutr. 124, 364-370.

Li, S., Yan, T., Yang, J. Q., Oberley, T. D., and Oberley, L. (2000) The role of cellular glutathione peroxidase redox regulation in the suppression of tumor cell growth by manganese superoxide dismutase. Cancer Res. 60, 3927-3939.

Liao, C. L., Huang, H. Y., Sheen, L. Y., and Chou, C. C. (2010) Anti-inflammatory activity of soymilk and fermented soymilk prepared with lactic acid bacterium and bifidobacterium. J. Food Drug Anal. 18, 202-210.

Liu, J. R., Lin, Y. Y., Chen, M. J., Chen, L. J., and Lin, C. W. (2005a) Antioxidative activities of kefir. Asian Australas. J. Anim. Sci. 18, 567-573. crossref(new window)

Liu, J. R., Chen, M. J., and Lin, C. W. (2005b) Antimutagenic and antioxidant properties of milk-kefir and soymilk-kefir. J Agr. Food Chem. 53, 2467-2474. crossref(new window)

Liu, J. R., Wang, S. Y., Chen, M.J., Chen, H. L., Yueh, P. Y., and Lin, C. W. (2006) Hypocholesterolaemic effects of milk-kefir and soyamilk-kefir in cholesterol-fed hamsters. Br. J. Nutr. 95, 939-946. crossref(new window)

Loo, A. Y, Jain, K., and Darah, I. (2007) Antioxidant and radical scavenging activities of the pyroligneous acid from a mangrove plant, Rhizophora apiculata. Food Chem. 104, 300-307. crossref(new window)

Madhuri, K. V. and Prabhakar, K. V. (2014) Microbial exopolysaccharides: biosynthesis and potential applications. Orient J. Chem. 30, 1401-1410. crossref(new window)

Mao, D. B., Shi, C. W., Wu, J. W., and Xu, C. P. (2014) Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity. Bioproc. Biosyst. Eng. 37, 1401-1409. crossref(new window)

Nikam, S., Nikam, P., Joshi, A., Viveki, R. G., Halappanavar, A. B., and Hungund, B. (2013) Effect of regular physical exercise (among circus athlets) on lipid profile, lipid peroxidation and enzymatic antioxidants. Int. J. Biochem. Res. Rev. 3, 414-420. crossref(new window)

Nugroho, A. E., Rais, I. R., Setiawan, I., Pratiwi, P. Y., Hadibarata, T., Tegar, M., and Pramono, S. (2014) Pancreatic effect of andrographolide isolated from Andrographis paniculata (Burm. F.) nees. Pak. J. Biol. Sci. 17, 22-31. crossref(new window)

Nurliyani, Harmayani, E., and Sunarti. (2014) Microbiological quality, fatty acid and amino acid profiles of kefir produced from combination of goat and soy milk. Pak. J. Nutr. 13, 107-115. crossref(new window)

Omayma, A. R., El-Sonbaty, S. M., Aziza, S. A., and Aboelftouh, A. E. (2013) Effect of probiotic fermented soy milk and gamma radiation on nitrosourea-induced mammary carcinogenesis. Nature Sci. 11, 35-42.

Oyedemi, S., Bradley, G., and Afolayan, A. (2012) Antidiabetic activities of aqueous stem bark extract of Strychnoshenningsii Gilg in streptozotocin-nicotinamide type 2 diabetic rats. Iran. J. Pharm. Res. 11, 221-228.

Poonuru, S., Pathak, S. R., Vats, H. S., and Pathak, R. D. (2011) Rapid reduction of severely elevated serum triglycerides with insulin infusion, gemfibrozil and niacin. J. Clin. Med. Res. 9, 38-41. crossref(new window)

Rani, V. U. and Pradeep, B. V. (2015) Antioxidant properties of soy milk fermented with Lactobacillus paracasei KUMB B005. Int. J. Pharm. Sci. Rev. Res. 30, 39-42.

Reeves, P. G., Neilsen, F. H., and Fahey, G. C. Jr. (1993) AIN-93 purified diets for laboratory rodents: Final report of the american institute of nutrition Ad Hoc writing committee on the formulation of the AIN-76A rodent diet. J. Nutr. 123, 1939-1951.

Rodríguez-Roque, M. J., Rojas-Graü, M. A., Elez-Martínez, P., and Martín-Belloso, O. (2013) Soymilk phenolic compounds, isoavones and antioxidant activity as affected by in vitro gastrointestinal digestion. Food Chem. 136, 206-212. crossref(new window)

Roselino, M. N., Pauly-Silveira, N. D., Cavallini, D. C. U., Celiberto, L. S., Pinto, R. A., Vendramini, R. C., and Rossi, E. A. (2012) A potential synbiotic product improves the lipid profile of diabetic rats. Lipids Health Dis. 11, 114. crossref(new window)

Rush University Medical Center, Chiago, Illinois. Normal Range for Common Laboratory Test.

Sharma, U., Sahu, R. K, Roy, A., and Golwala, D. K. (2010) In vivo antidiabetic and antioxidant potential of Stephania hernandifolia in streptozotocin-induced-diabetic rats. J. Young Pharm. 2, 255-260. crossref(new window)

SPSS (2007) SPSS Statistics Base 17.0 User’s Guide. SPSS Inc. 233 South Wacker Drive, 11th Floor. Chicago, IL 60606-6412.

Srinivasan, K., Viswanad, B., Asrat, L., Kaul C. L., and Ramarao, P. (2005) Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: A model for type 2 diabetes and pharmacological screening. Pharmacol. Res. 52, 313-320. crossref(new window)

Szkudelski, T. (2012) Streptozotocin-nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Exp. Biol. Med. 237, 481-490. crossref(new window)

Vinderola, G., Perdigón, G., Duarte J., Farnworth, E., and Matar, C. (2006) Effects of the oral administration of the exopolysaccharide produced by Lactobacillus kefiranofaciens on the gut mucosal immunity. Cytokine 36, 254-260. crossref(new window)

WHO (1999) Definition, diagnosis and classification of diabetes mellitus and its complications report of a WHO consultation Part 1: Diagnosis and classification of diabetes mellitus. World Health Organization, Department of Non communicable Disease Surveillance, Geneva.

Wong, C. W. and Watson, D. L. (1995) Immunomodulatory effects of dietary whey proteins in mice. J. Dairy Res. 62, 359-368. crossref(new window)

Yadav, H., Jain, S., and Sinha, P. R. (2008) Oral administration of dahi containing probiotic Lactobacillus acidophilus and Lactobacillus casei delayed the progression of streptozotocin-induced diabetes in rats. J. Dairy Res. 75, 189-195.

Yin, J., Heo, S. I., and Wang, M. H. (2008) Antioxidant and antidiabetic activities of extracts from Cirsium japonicum roots. Nutr. Res. Pract. 2, 247-251. crossref(new window)

Yoon, G. A. and Park, S. (2014) Antioxidant action of soy isoflavones on oxidative stress and antioxidant enzyme activities in exercised rats. Nutr. Res. Pract. 8, 618-624. crossref(new window)

Zafar, M. and Naqvi, S. N. H. (2010) Effects of STZ-induced diabetes on the relative weights of kidney, liver and pancreas in Albino rats: A comparative study. Int. J. Morphol. 28, 135-142.

Zimecki, M. and Kruzel, M. L. (2000) Systemic or local coadministeration of lactoferrin with sensitizing dose of antigen enhanches delayed type hypersensitivity in mice. Immunol. Lett. 74, 183-188. crossref(new window)

Zommara, M., Toubo, H., Sakono, M., and Imaizumi, K. (1998) Prevention of peroxidative stress in rats fed on a low vitamin e-containing diet by supplementing with a fermented bovine milk whey preparation: effect of lactic acid and β-lactoglobulin on the antiperoxidative action. Biosci. Biotechnol. Biochem. 62, 710-717. crossref(new window)