JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Screening of Cholesterol-lowering Bifidobacterium from Guizhou Xiang Pigs, and Evaluation of Its Tolerance to Oxygen, Acid, and Bile
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Screening of Cholesterol-lowering Bifidobacterium from Guizhou Xiang Pigs, and Evaluation of Its Tolerance to Oxygen, Acid, and Bile
Zhang, Rujiao; He, Laping; Zhang, Ling; Li, Cuiqin; Zhu, Qiujin;
  PDF(new window)
 Abstract
Cardiovascular and cerebrovascular diseases seriously harm human health, and Bifidobacterium is the most beneficial probiotic in the gastrointestinal tract of humans. This work aimed to screen cholesterol-lowering Bifidobacterium from Guizhou Xiang Pig and evaluate its tolerance to oxygen, acid, and bile. Twenty-seven aerotolerant strains with similar colony to Bifidobacterium were isolated through incubation at 37℃ in 20% (v/v) CO2-80% (v/v) atmospheric air by using Mupirocin lithium modified MRS agar medium, modified PTYG with added CaCO3, and modified PTYG supplemented with X-gal. Ten strains with cholesterol-lowering rates above 20% (w/w) were used for further screening. The selected strains’ tolerance to acid and bile was then determined. A combination of colony and cell morphology, physiological, and biochemical experiments, as well as 16S rRNA gene-sequence analysis, was performed. Results suggested that BZ25 with excellent characteristics of high cholesterol-removal rate of 36.32% (w/w), as well as tolerance to acid and bile, was identified as Bifidobacterium animalis subsp. lactis. To further evaluate Bifidobacterium BZ25’s growth characteristic and tolerance to oxygen, culture experiments were performed in liquid medium and an agar plate. Findings suggested that BZ25 grew well both in environmental 20% (v/v) CO2-80% (v/v) atmospheric air and in 100% atmospheric air because BZ25 reached an absorbance of 1.185 at 600 nm in 100% atmospheric air. Moreover, BZ25 was aerotolerant and can grow in an agar medium under the environmental condition of 100% atmospheric air. This study can lay a preliminary foundation for the potential industrial applications of BZ25.
 Keywords
Screening;Bifidobacterium;cholesterol;identification;tolerance to acid;bile;and oxygen;
 Language
English
 Cited by
 References
1.
Ahn, J., Hwang, H., and Park, J. (2001) Physiological responses of oxygen-tolerant anaerobic Bifidobacterium longum under oxygen. J. Microbiol. Biotechnol. 11, 443-451.

2.
Andriantsoanirina, V., Allano, S., Butel, M., and Aires, J. (2013) Tolerance of Bifidobacterium human isolates to bile, acid and oxygen. Anaerobe 21, 39-42. crossref(new window)

3.
Cai, Y., Matsumoto, M., and Benno, Y. (2000) Bifidobacterium lactis meile, et al. 1997 is a subjective synonym of Bifidobacterium animalis (mitsuoka 1969) scardovi and trovatelli 1974. Microbiol. Immunol. 44, 815-820. crossref(new window)

4.
Oh, C. Y. and Lee, W. K. (2000) Cholesterol lowering effect of lactic acid bacteria isolated from the human intestine. Korean J. Vet. Public Health 24, 181-188.

5.
Dunne, C. (2001) Adaptation of bacteria to the intestinal niche: probiotics and gut disorder. Inflamm. Bowel. Dis. 7, 136-145. crossref(new window)

6.
Jungersen, M., Wind, A., Johansen, E., Christensen, J. E., Stuer-Lauridsen, B., and Eskesen, D. (2014) The science behind the probiotic strain Bifidobacterium animalis subsp. lactis BB-12®. Microorganisms 2, 92-110. crossref(new window)

7.
Kawasaki, S., Mimura, T., Satoh, T., Takeda, K., and Niimura, Y. (2006) Response of the microaerophilic Bidobacterium species, B. boum and B. thermophilum, to oxygen. Appl. Environ. Microbiol. 72, 6854-6858. crossref(new window)

8.
Liong, M. T. and Shah, N. P. (2005) Bile salt deconjugation and bsh activity of five Bifidobacterial strains and their cholesterol co-precipitating properties. Food Res. Int. 38, 135-142. crossref(new window)

9.
Liu, Y., Tang, H., Lin, Z., and Xu, P. (2015) Mechanisms of acid tolerance in bacteria and prospects in biotechnology and bioremediation. Biotechnol. Adv. 33, 1484-1492. crossref(new window)

10.
De Man, J. C., Rogosa, M., and Sharpe, M. E. (1960) A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23, 130-135. crossref(new window)

11.
Mayer, H., Amtmann, E., Philippi, E., Steinegger, G., Mayrhofer, S., and Kneifel, W. (2007) Molecular discrimination of new isolates of Bifidobacterium animalis subsp. lactis from reference strains and commercial probiotic strains. Int. Dairy J. 17, 565-573. crossref(new window)

12.
Meile, L., Ludwig, W., Rueger, U., Gut, C., Kaufmann, P., Dasen, G., Wenger, S., and Teuber, M. (1997) Bifidobacterium lactis sp. nov, a moderately oxygen tolerant species isolated from fermented milk. System. Appl. Microbiol. 20, 57-64. crossref(new window)

13.
Nishida, S., Gotou, M., Akutsu, S., Ono, M., Hitomi, Y., Nakamura, T., and Iino, H. (2004) Effect of yoghurt containing Bifidobacterium lactis Bb-12® on improvement of defaecation and faecal microflora of healthy female adults. Milk Sci. 53, 71-80.

14.
Rudel, L. L. and Morris, M. D. (1973) Determination of cholesterol using o-phthalaldehyde. J. Lipid Res. 14, 364-366.

15.
Sánchez, B., Reyes-Gavilán, C. D. L., and Margolles, A. (2006) The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance. Environmental Microbiology 8, 1825-1833. crossref(new window)

16.
Sánchez, B., Ruiz, L., Reyes-Gavilán, C. D. L., and Margolles, A. (2008) Proteomics of stress response in Bifidobacterium. Front Biosci. 1, 6905-6919.

17.
Sánchez, B., Champomier-Vergès, M., Collado, M., Anglade, P., Baraige, F., Sanz, Y., Reyes-Gavilán, C. D. L., Margolles, A., and Zagorec, M. (2007) Low-pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum. Appl. Environ. Microbiol. 73, 6450-6459. crossref(new window)

18.
Shin, S. Y. and Park, J. H. (1997) Activities of oxidative enzymes related with oxygen tolerance in Bifidobacterium sp. J. Microbiol. Biotechnol. 7, 356-359.

19.
Steinberg, D., Parthasarathy, S., Carew, T., Khoo, J., and Witztum, J. (1989) Beyond cholesterol modification of low density lipoprotein that increase its atherogenicity. N. Engl. J. Med. 320, 915-924. crossref(new window)

20.
WHO (2009) Cardiovascular disease. http://www.Who.Int/mediacentre /factsheets/fs317/en/print.html.

21.
Yu, Z., Zhang, X., Li, S., Li, C., Li, D., and Yang, Z. (2013) Evaluation of probiotic properties of Lactobacillus plantarum strains isolated from chinese sauerkraut. World J. Microbiol. Biotechnol. 29, 489-498. crossref(new window)

22.
Zheng, Y., Lu, Y., Wang, J., Yang, L., Pan, C., and Huang, Y. (2013) Pro-biotic properties of Lactobacillus strains isolated from tibetan kefir grains. PLoS ONE 8, 10.1371/journal.pone.0069868. crossref(new window)

23.
Ziarno, M., Sekul, E., and Lafraya, A. A. (2007) Cholesterol assimilation by commercial yoghurt starter cultures. Acta Sci. Pol. Technol. 6, 83-94.