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Establishing Quantitative Standards for Residual Alkaline Phosphatase in Pasteurized Milk
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 Title & Authors
Establishing Quantitative Standards for Residual Alkaline Phosphatase in Pasteurized Milk
Kim, Dong-Hyeon; Chon, Jung-Whan; Lim, Jong-Soo; Kim, Hong-Seok; Kang, Il-Byeong; Jeong, Dana; Song, Kwang-Young; Kim, Hyunsook; Kim, Kwang-Yup; Seo, Kun-Ho;
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The alkaline phosphatase (ALP) assay is a rapid and convenient method for verifying milk pasteurization. Since colorimetric ALP assays rely on subjective visual assessments, their results are especially unreliable near the detection limits. In this study, we attempted to establish quantitative criteria for residual ALP in milk by using a more objective method based on spectrophotometric measurements. Raw milk was heat-treated for 0, 10, 20, 30, and 40 min and then subjected to ALP assays. The quantitative criteria for residual ALP in the milk was determined as 2 μg phenol/mL of milk, which is just above the ALP value of milk samples heat-treated for 30 min. These newly proposed methodology and criteria could facilitate the microbiological quality control of milk.
alkaline phosphatase;milk;pasteurization;quantitative criteria;spectrophotometer;
 Cited by
AOAC international (2000) Official methods of analysis. 17th ed, AOAC International, Arlington, VA, sec. 946.01.

Fanni, J. A. (1983) Phosphohydrolase activities of Penicillium caseicolum. Milchwissenschaft 38, 523-526.

Hammer, B. W. and Olson, H. C. (1941) Phosphatase production in dairy products by microorganisms. J. Milk Technol. 4, 83-85.

Klotz, V., Hill, A., Warriner, K., Griffiths, M., and Odumeru, J. (2008) Assessment of the colorimetric and fluorometric assays for alkaline phosphatase activity in cow's, goat's, and sheep's milk. J. Food Prot. 71, 1884-1888. crossref(new window)

Korea Food and Drug Administration. Food code. Available from: Accessed Mar. 3, 2015.

Murthy, G. K., Kleyn, D. H., Richardson, T., and Rocco, R. M. (1992) Alkaline phosphatase methods. In: Standard methods for the examination of dairy products. Marshall, R. T. (ed) American Public Health Association, Washington, DC, pp. 413-431.

Rankin, S. A., Christiansen, A., Lee, W., Banavara, D. S., and Lopez-Hernandez, A. (2010) Invited review: The application of alkaline phosphatase assays for the validation of milk product pasteurization. J. Dairy Sci. 93, 5538-5551. crossref(new window)

Schlimme, E., Kiesner, C., Lorenzen, P. C., and Martin, D. (1997) Chemical process parameters for thermal inactivation of alkaline phosphatase in milk. Kieler Milchw. Forsch. 49, 207-219.

Suzuki, U., Yoshimura, K., and Takaishi, M. (1907) Uber ein enzyme “phytase” das anhydro-oxy-methylen-diphosphorsaure spaltet. Bull. Coll. Agric. Tokyo Imp. Univ. 7, 503-512.

United States Food and Drugs Administration (2001) BAM: Screening method for phosphatase in Cheese. Available from: Accessed Jun. 12, 2015.