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Detection for Non-Milk Fat in Dairy Product by Gas Chromatography
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 Title & Authors
Detection for Non-Milk Fat in Dairy Product by Gas Chromatography
Kim, Ha-Jung; Park, Jung-Min; Lee, Jung-Hoon; Kim, Jin-Man;
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The aim of this study was to evaluate the potential use of fatty acids, triacylglycerols, and cholesterol in the detection of adulterated milk fat. The fatty acid, triacylglycerol, and cholesterol profiles of the mixtures of milk and non-milk fat (adulteration ratios of 10%, 30%, 50%, 70%, and 90%) were analyzed by gas chromatography. The results showed that concentrations of the fatty acids with oleic acid (C18:1n9c) and linoleic acid (C18:2n6c), triglycerides with C52 and C54, and cholesterol detected are proportional to the adulteration ratios remarkably. Oleic acid (C18:1n9c), linoleic acid (C18:2n6c), C52, and C54 were lower in pure milk fat than in adulterated mixtures. In contrast, pure milk has a higher cholesterol concentration than all adulterated mixtures (adulteration concentration in the range 10-90%). Thus, we suggest that oleic acid (C18:1n9c), linoleic acid (C18:2n6c), C52, C54, and cholesterol are suitable indicators and can be used as biomarkers to rapidly detect adulterated milk fat by gas chromatography. This study is expected to provide basic data for adulteration and material usage. Moreover, this new approach can detect the presence of foreign oils and fats in the milk fat of cheese and can find application in related studies.
adulteration;milk fat;cheese;gas chromatography;foreign fat;
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한국축산식품학회지, 2016. vol.36. 5, pp.612-616 crossref(new window)
Challinor, J. M. (1996) A rapid simple pyrolysis derivatisation gas chromatography-mass spectrometry method for profiling of fatty acids in trace quantities of lipids. J. Anal. Appl. Pyrol. 37, 185-197. crossref(new window)

Destaillats, F., Wispelaere, M., Joffre, F., Golay, P. A., Huga, B., Giuffrida, F., Fauconnot, L., and Dionisi, F. (2006) Authenticity of milk fat by fast analysis of triacylglycerols application to the detection of partially hydrogenated vegetable oils. J. Chromatogr. A 1131, 227-234. crossref(new window)

Fontecha, J., Mayo, I., Toledano, G., and Juárez, M. (2006) Use of changes in triacylglycerols during ripening of cheeses with high lipolysis levels for detection of milk fat authenticity. Int. Dairy J. 16, 1498-1504. crossref(new window)

Goudjil, H., Fontecha, J., Fraga, M. J., and Juarez, M. (2003) TAG composition of ewe’s milk fat. Detection of foreign fats. J. Am. Oil Chem. Soc. 80, 219-222. crossref(new window)

Gutiérrez, R., Vega, S., Díaz, G., Sánchez, J., Coronado, M., Ramírez, A., Pérez, J., González, M., and Schettino, B. (2009) Detection of non-milk fat in milk fat by gas chromatography and linear discriminant analysis. J. Dairy Sci. 92, 1846-1855. crossref(new window)

Hocquette, J. F. and Gigli, S. (2005) Indicators of milk and beef quality. Wageningen Academic Publishers. Wageningen, Netherlands, p. 38.

Hurley, I. P., Coleman, R. C., Ireland, H. E., and Williams, J. H. H. (2006) Use of sandwich IgG ELISA for the detection and quantification of adulteration of milk and soft cheese. Int. Dairy J. 16, 805-812. crossref(new window)

Hurley, I. P., Ireland, H. E., Coleman, R. C., and Williams, J. H. H. (2004) Application of immunological methods for the detection of species adulteration in dairy products. Int. J. Food Sci. Technol. 39, 873-878. crossref(new window)

International Dairy Federation (IDF) (2008). Nutritional quality of milkfat. Available from: Accessed Mar. 03, 2014.

Kim, N. S., Lee, J. H., Han, K. M., Kim, J. W., Cho, S., and Kim, J. (2013) Discrimination of commercial cheeses from fatty acid profiles and phytosterol contents obtained by GC and PCA. Food Chem. 15, 40-47.

Lipp, M. (1996) Determination of the adulteration of butter fat by its triglyceride composition obtained by GC. A comparison of the suitability of PLS and neural networks. Food Chem. 55, 389-395. crossref(new window)

Marekov, I., Nedelcheva, D., Panayotova, S. and Tarandjiiska, R. (2011) Detection of milkfat adulteration by GC analysis of saturated, CIS-monoenoic and CIS, CIS-dienoic fatty acid fractions isolated by silver ion TLC. J. Liq. Chromatogr. Relat. Technol. 34, 888-901. crossref(new window)

Miller, G. D., Jarvis, J. K., and McBean, L. D. (2006) Handbook of dairy foods and nutrition. 3rd ed, Taylor & Francis, NY, p. 432.

Ministry of Food and Drug Safety (MFDS) (2014) Food code. Available from: Accessed Jan. 30, 2014.

Moatsou, G. and Anifantakis, E. (2003) Recent developments in antibody-based analytical methods for the differentiation of milk from different species. Int. J. Dairy Technol. 56, 133-138. crossref(new window)

Molkentin, J. (2007) Detection of foreign fat in milk fat from different continents by triacylglycerol analysis. Eur. J. Lipid Sci. Technol. 109, 505-510. crossref(new window)

Molkentin, J. and Precht, D. (1998) Comparison of gas chromatographic methods for analysis of butyric acid in milk fat and fats containing milk fat. Z. Lebensm. Unters. Forsch. 206, 213-216. crossref(new window)

Ntakatsane, M. P., Liu, X. M., and Zhou, P. (2013) Short communication: Rapid detection of milk fat adulteration with vegetable oil by fluorescence spectroscopy. J. Dairy Sci. 96, 2130-2136. crossref(new window)

Park, J. M., Jeong, I. S., Kwak, B. M., Ahn, J. H., Leem, D. G., Jeong, J. Y., and Kim, J. M. (2013) Application of rapid sample preparation method and monitoring for cholesterol content in chicken egg and egg powder. Korean J. Food Sci. An. 33, 672-677. crossref(new window)

Parodi, P. W. (1971) Detection of synthetic and adulterated butterfats: III. Triglyceride and fatty acid analysis. J. Dairy Technol. 26, 155-158.

Pereira, P. C. (2014) Milk nutritional composition and its role in human health. Nutrition. 30, 619-627. crossref(new window)

Pinto, C. M., Contreras, M. O., Carrasco, R. E., Brito, C. C., Molina, H. C. L., Ah-hen, S. K., and Vega, S. L. (2002) Determinación de la autenticidad de grasas lácteas. Análisis discriminante lineal de triacilglicéridos. Agro Sur. 30, 59-67. crossref(new window)