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Kinetic Behavior of Salmonella on Low NaNO2 Sausages during Aerobic and Vacuum Storage
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 Title & Authors
Kinetic Behavior of Salmonella on Low NaNO2 Sausages during Aerobic and Vacuum Storage
Ha, Jimyeong; Gwak, Eunji; Oh, Mi-Hwa; Park, Beomyoung; Lee, Jeeyeon; Kim, Sejeong; Lee, Heeyoung; Lee, Soomin; Yoon, Yohan; Choi, Kyoung-Hee;
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This study evaluated the growth kinetics of Salmonella spp. in processed meat products formulated with low sodium nitrite (NaNO2). A 5-strain mixture of Salmonella spp. was inoculated on 25-g samples of sausages formulated with sodium chloride (NaCl) (1.0%, 1.25%, and 1.5%) and NaNO2 (0 and 10 ppm) followed by aerobic or vacuum storage at 10℃ and 15℃ for up to 816 h or 408 h, respectively. The bacterial cell counts were enumerated on xylose lysine deoxycholate agar, and the modified Gompertz model was fitted to the Salmonella cell counts to calculate the kinetic parameters as a function of NaCl concentration on the growth rate (GR; Log CFU/g/h) and lag phase duration (LPD; h). A linear equation was then fitted to the parameters to evaluate the effect of NaCl concentration on the kinetic parameters. The GR values of Salmonella on sausages were higher (p<0.05) with 10 ppm NaNO2 concentration than with 0 ppm NaNO2. The GR values of Salmonella decreased (p<0.05) as NaCl concentration increased, especially at 10℃. This result indicates that 10 ppm NaNO2 may increase Salmonella growth at low NaCl concentrations, and that NaCl plays an important role in inhibiting Salmonella growth in sausages with low NaNO2.
Salmonella;kinetic model;NaCl;NaNO2;sausages;
 Cited by
Aguilera, J. M. and Karel, M. (1997) Preservation of biological materials under desiccation. Crit. Rev. Food Sci. Nutr. 37, 287-309. crossref(new window)

Bearson, S., Bearson, B., and Foster, J. W. (1997) Acid stress responses in enterobacteria. FEMS Microbiol. Lett. 147, 173-180. crossref(new window)

Birk, T., Henriksen, Müller, Hansen, T. B., and Aabo, S. (2015) Growth potential of exponential- and stationary-phase Salmonella Typhimurium during sausage fermentation. Meat Sci. 116 (in press)

Bonnet, M. and Montville, T. J. (2005) Acid-tolerant Listeria monocytogenes persist in a model food system fermented with nisin-producing bacteria. Lett. Appl. Microbiol. 40, 237-242. crossref(new window)

Choi, Y. S., Kim, H. W., Hwang, K. E., Song, D. H., Choi, J. H., Lee, M. A., Chung, H. J., and Kim, C. J. (2014) Physicochemical properties and sensory characteristics of reducedfat frankfurters with pork back fat replaced by dietary fiber extracted from makgeolli lees. Meat Sci. 96, 892-900. crossref(new window)

Davies, R., Paiba, G., Evans, S., and Dalziel, B. (2000) Surveys for Salmonella in pigs, cattle and sheep at slaughter in Great Britain. Vet. Rec. 147, 695.

Gibson, A. M., Bratchell, N., and Roberts, T. A. (1987) The effect of sodium chloride and temperature on the rate and extent of growth of Clostridium botulinum type A in pasteurized pork slurry. J. Appl. Bacteriol. 62, 479-490. crossref(new window)

Gilberthorpe, N. J. and Poole, R. K. (2008) Nitric oxide homeostasis in Salmonella typhimurium roles of respiratory nitrate reductase and flavohemoglobin. J. Biol. Chem. 283, 11146-11154. crossref(new window)

Ham, H., Hong, I., Lim, H., Yang, Y., Choi, Y., Kim, C., Kweon, T., and Lee, J. (2004) Nitrites contents on processed meat products (ham, sausage etc) in market during 2000-2003. Korean J. Vet. Serv. 27, 115-120.

Horsch, A. M., Sebranek, J. G., Dickson, J. S., Niebuhr, S. E., Larson, E. M., Lavieri, N. A., and Wilson, L. A. (2014) The effect of pH and nitrite concentration on the antimicrobial impact of celery juice concentrate compared with conventional sodium nitrite on Listeria monocytogenes. Meat Sci. 96, 400-407. crossref(new window)

Jo, H., Park, B., Oh, M., Gwak, E., Lee, H., Lee, S., and Yoon, Y. (2014) Probabilistic models to predict the growth initiation time for Pseudomonas spp. in processed meats formulated with NaCl and NaNO2. Korean J. Food Sci. An. 34, 736-741. crossref(new window)

Junttila, J., Hirn, J., Hill, P., and Nurmi, E. (1989) Effect of different levels of nitrite and nitrate on the survival of Listeria monocytogenes during the manufacture of fermented sausage. J. Food. Protect 52, 158-161.

Krause, B. L., Sebranek, J. G., Rust, R. E., and Mendonca, A. (2011) Incubation of curing brines for the production of readyto- eat, uncured, no-nitrite-or-nitrate-added, ground, cooked and sliced ham. Meat Sci. 89, 507-513. crossref(new window)

Long, S. M., Adak, G. K., O'Brien, S. J., and Gillespie, I. A. (2002) General outbreaks of infectious intestinal disease linked with salad vegetables and fruit, England and Wales, 1992- 2000. Commun. Dis. Public Health 5, 101-105.

Mills, P. C., Rowley, G., Spiro, S., Hinton, J. C., and Richardson, D. J. (2008) A combination of cytochrome c nitrite reductase (NrfA) and flavorubredoxin (NorV) protects Salmonella enterica serovar Typhimurium against killing by NO in anoxic environments. Microbiology 154, 1218-1228. crossref(new window)

Nichols, G. L. and De Louvois, J. (1995) The microbiological quality of raw sausages sold in the UK. PHLS Microbiol. Dig. 12, 236-242.

Page, G. V. and Solberg, M. (1980) Nitrogen assimilation by Salmonella typhimurium in a chemically defined minimal medium containing nitrate, nitrite, or ammonia. J. Food Sci. 45, 75-76. crossref(new window)

Poole, R. K. and Hughes, M. N. (2000) New functions for the ancient globin family: Bacterial responses to nitric oxide and nitrosative stress. Mol. Microbiol. 36, 775-783. crossref(new window)

Rhee, K. S. and Ziprin, Y. A. (2001) Pro-oxidative effects of NaCl in microbial growth-controlled and uncontrolled beef and chicken. Meat Sci. 57, 105-112. crossref(new window)

Seong, P. N., Kim, J. H., Cho, S. H., Kang, D. W., Kang, G. H., Park, B. Y., Lee, J. M., Jung, J. H., and Kim, D. H. (2010) The effects of salt and NaNO2 on fatty acid composition, free amino acids, microbial counts and sensory characteristics of dry-cured ham processed under Korean environment. Korean J. Food Sci. An. 30, 435-442. crossref(new window)

Sindelar, J. J., Cordray, J. C., Sebranek, J. G., Love, J. A., and Ahn, D. U. (2007) Effects of varying levels of vegetable juice powder and incubation time on color, residual nitrate and nitrite, pigment, pH, and trained sensory attributes of ready-to-eat uncured ham. J. Food Sci. 72, S388-S395. crossref(new window)

Sugimura, T. (2000) Nutrition and dietary carcinogens. Carcinogenesis 21, 387-395. crossref(new window)

Uyttendaele, M., De Troy, P., and Debevere, J. (1999) Incidence of Salmonella, Campylobacter jejuni, Campylobacter coli, and Listeria monocytogenes in poultry carcasses and different types of poultry products for sale on the Belgian retail market. J. Food Protect. 62, 735-740.

Wagner, C. and Hensel, M. (2011) Adhesive mechanisms of Salmonella enterica. Adv. Exp. Med. Biol. 715, 17-34. crossref(new window)

Whiting, R. C. and Buchanan, R. L. (1997) Development of a quantitative risk assessment model for Salmonella enteritidis in pasteurized liquid eggs. Int. J. Food Microbiol. 36, 111-125. crossref(new window)

Yoon, Y. (2010) Principal theory and application of predictive microbiology. Food Sci. Ind. 43, 70-74.

Zwietering, M. H., Jongenburger, I., Rombouts, F. M., and Van't Riet, K. (1990) Modeling of the bacterial growth curve. Appl. Environ. Microbiol. 56, 1875-1881.