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Ethanol Extract of Ulmus pumila Ameliorates Heat Stress through the Induction of Heat Shock Proteins Expression in RAW264.7 Macrophage Cells
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  • Journal title : Journal of Animal Environmental Science
  • Volume 20, Issue 4,  2014, pp.147-154
  • Publisher : Korean Association for Livestock Housing and Environment
  • DOI : 10.11109/JAES.2014.20.4.147
 Title & Authors
Ethanol Extract of Ulmus pumila Ameliorates Heat Stress through the Induction of Heat Shock Proteins Expression in RAW264.7 Macrophage Cells
dela Cruz, Joseph; Byambaragchaa, Munkhzaya; Choi, Seok-Geun; Hwang, Seong-Gu;
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Heat stress is a significant burden to animal production in most areas of the world. Improving our knowledge of physiological and metabolic mechanisms of acclimation may contribute to the development of procedures that may help to maintain health and production efficiency under hot temperature. The effect of Ulmus pumila (UP) extract in inducing Heat Shock Proteins (HSPs) expression in heat-stressed RAW264.7 macrophage cells was investigated. Cell viability assay showed a dose dependent increase in cells after treatment with UP for 24 hours. RT-PCR and western blot analysis showed that increasing concentrations of UP induce the expression of Heat Shock Factor 1 (HSF1) and dose dependently upregulated the expression of Heat shock protein 70 (Hsp70) and Hsp90. LPS-induced nitric oxide was dose-dependently reduced while phagocytic activity greatly recovered with UP treatment. These data demonstrated that UP can be a potential candidate in the development of cytoprotective agent against heat stress.
RAW264.7 cells;Ulmus pumila;heat stress;Hsp70;Hsp90;
 Cited by
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