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Application of Next Generation Sequencing to Investigate Microbiome in the Livestock Sector
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  • Journal title : Journal of Animal Environmental Science
  • Volume 21, Issue 3,  2015, pp.93-98
  • Publisher : Korean Association for Livestock Housing and Environment
  • DOI : 10.11109/JAES.2015.21.3.93
 Title & Authors
Application of Next Generation Sequencing to Investigate Microbiome in the Livestock Sector
Kim, Minseok; Baek, Youlchang; Oh, Young Kyoon;
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The objective of this study was to review application of next-generation sequencing (NGS) to investigate microbiome in the livestock sector. Since the 16S rRNA gene is used as a phylogenetic marker, unculturable members of microbiome in nature or managed environments have been investigated using the NGS technique based on 16S rRNA genes. However, few NGS studies have been conducted to investigate microbiome in the livestock sector. The 16S rRNA gene sequences obtained from NGS are classified to microbial taxa against the 16S rRNA gene reference database such as RDP, Greengenes and Silva databases. The sequences also are clustered into species-level OTUs at 97% sequence similarity. Microbiome similarity among treatment groups is visualized using principal coordinates analysis, while microbiome shared among treatment groups is visualized using a venn diagram. The use of the NGS technique will contribute to elucidating roles of microbiome in the livestock sector.
16S rRNA gene;Livestock sector;Microbiome;Next-generation sequencing;
 Cited by
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