Advanced SearchSearch Tips
Effects of Haedokgumhwa-san Water Extracts on LPS-induced Inflammatory Response in Macrophage
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : The Korea Journal of Herbology
  • Volume 30, Issue 5,  2015, pp.67-74
  • Publisher : The Korea Association of Herbology
  • DOI : 10.6116/kjh.2015.30.5.67.
 Title & Authors
Effects of Haedokgumhwa-san Water Extracts on LPS-induced Inflammatory Response in Macrophage
LIM, Jae-Soo; KANG, Ok-Hwa; SEO, Yun-Soo; KWON, Dong-Yeul;
  PDF(new window)
Objectives : TheHaedokgumhwa-sanwater extract (HDKHS) is used in Korea, Japan and China as a traditional therapeutic agent to cure an infectious disease. But its study is not enough. Therefore, the present study focused on the elucidation of HDKHS to investigate the anti-inflammatory effects and to established the possible mechanisms involved in its action on LPS-stimulated immune response in murine macrophages.Methods : Inflammatory status was induced by LPS and measured by increasement of inflammatory mediators. LPS induced secretions of NO and PGE2in RAW 264.7 cells were measured using griess reagent and enzyme-linked immunosorbent assay (ELISA) kit respectively. production of IL-6 was examined using ELISA kit and expression of IL-6 mRNA was measured by RT-PCR method. To investigate the effects of HDKHS on inflammatory mediators, such as iNOS, COX-2 and MAPKs, western blot and RT-PCR were performed.Results : HDKHS significantly reduced production of NO and PGE2 which were induced by LPS. Also, activation of IL-6 was reduced both protein and mRNA levels. The expressions of inflammatory mediator include iNOS and COX-2 were decreased by pretreatment with HDKHS. futhermore The result showed HDKHS down-regulate the LPS induced phosphorylation of ERK 1/2, one of the MAPK family, which is considered as a main regulator of transmission from pathogens to nucleus of immune cells.Conclusions : Our results suggest that the anti-inflammatory properties of HDKHS may stem from the inhibition of pro-inflammatory mediators via suppression of initiation of inflammatory response by inhibiting MAPKs signaling pathways.
Haedokgumhwa-san;Anti-inflammatory activity;Macrophages;Mitogen-activated protein kinases (MAPKs);
 Cited by
가미생맥산(加味生脈散) 및 개별약재의 항산화 및 항염증 효능에 대한 비교 연구,지중구;

대한본초학회지, 2016. vol.31. 1, pp.69-75 crossref(new window)
Wang HT, Fang YQ, You P, Bao XC, Yuan HR, Ma J, Wang FF, Li KC. Expression changes of inflammatory factors in the rat lung of decompression sickness induced by fast buoyancy ascent escape. Undersea Hyperb Med. 2015 ; 42(1) : 15-22.

Lomba KS, de Souza Breves Beiler TF, Sete MR, Pires FR, da Silva Figueredo CM. Use of minimally invasive gingival biopsies in the study of inflammatory mediators expression and their correlation with gingival fluid in patients with chronic periodontitis. Indian J Dent Res. 2015 ; 26(2) : 126-30. crossref(new window)

Bae BC. Commentary on Nei-Jing. Seoul : Sungbosa. 200, 725-8.

Donguihak Dictionary Compilation Committee. New donguihak Dictionary. seoul : Yeo Gangchulpansa. 2003 : 218.

Mum RM. Documentary Consideration about Stress. J Orient Neuropsychiatry. 1991 ; 2(1) : 38-50.

Ju JH. Shinpyeon Dangyesimbeopbuyeo(sang). seoul : Dae Seong munhwasa. 1993 : 255.

Heo J. Donguibogam. 9th ed. Seoul : Namsandang. 2009 : 188.

Heo J. Donguibogam. 9th ed. Seoul : Namsandang. 2009 : 396.

Heo J. Donguibogam. 9th ed. Seoul : Namsandang. 2009 : 182.

Lee HI, Lee SK, Kwon YM, Song YS. Antibacterial effect of Haedokgeumhwa-san against methicillin-resistant Staphylococcus aureus. J Orient Rehabilitation Med. 2015 ; 25(2) : 1-13. crossref(new window)

McNelis JC, Olefsky JM. Macrophages, immunity, and metabolic disease. Immunity. 2014 ; 41(1) : 36-48. crossref(new window)

Predonzani A, Calì B, Agnellini AH, Molon B. Spotlights on immunological effects of reactive nitrogen species: When inflammation says nitric oxide. World J Exp Med. 2015 ; 5(2) : 64-76. crossref(new window)

Lee JK. Analgesic and anti-inflammatory effect of Scutellaria baicalensis. Kyung Won University. 2008.

Puddu P, Latorre D, Valenti P, Gessani S. Immunoregulatory role of lactoferrin-lipopolysaccharide interactions. Biometals. 2010 ; 23(3) : 387-97. crossref(new window)

Fang H, Pengal RA, Cao X, Ganesan LP, Wewers MD, Marsh CB, Tridandapani S. Lipopolysaccharide -induced macrophage inflammatory response is regulated by SHIP. J Immunol 2004 ; 173 : 360-6. crossref(new window)

Jang SI, Kim BH, Lee WY, An SJ, Choi HG, Jeon BH, Chung HT, Rho JR, Kim YJ, Chai KY. Stylopine from Chelidonium majus inhibits LPS-induced inflammatory mediators in RAW 264.7 cells. Arch Pharm Res. 2004 ; 27(9) : 923-9. crossref(new window)

Kim JB, Han AR, Park EY, Kim JY, Cho W, Lee J, Seo EK, Lee KT. Inhibition of LPS-induced iNOS, COX-2 and cytokines expression by poncirin through the NF-kappaB inactivation in RAW 264.7 macrophage cells. Biol Pharm Bull 2007 ; 30(12) : 2345-51. crossref(new window)

Aalaei-andabili SH, Rezaei N. Toll like receptor (TLR)-induced differential expression of microRNAs (MiRs) promotes proper immune response against infections: a systematic review. J Infect. 2013 ; 67(4) : 251-64. crossref(new window)

Weidinger A, Kozlov AV. Biological Activities of Reactive Oxygen and Nitrogen Species: Oxidative Stress versus Signal Transduction. Biomolecules. 2015 ; 5(2) : 472-84. crossref(new window)

Ryu JH, Ahn H, Kim JY, Kim YK. Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-actived macrophage. Phytother Res. 2003 ; 17(5) : 485-9. crossref(new window)

Radulovic M, Bauman WA, Wecht JM, LaFountaine M, Kahn N, Hobson J, Singh K, Renzi C, Yen C, Schilero GJ. Biomarkers of inflammation in persons with chronic tetraplegia. J Breath Res. 2015 ; 9(3) : 036001. crossref(new window)

Kobayashi Y. The regulatory role of nitric oxide in proinflammatory cytokine expression during the induction and resolution of inflammation. J Leukoc Biol. 2010 ; 88(6) : 1157-62. crossref(new window)

Kuo CL, Chi CW, Liu TY. The anti-inflammatory potential of berberine in vitro and in vivo. Cancer letters. 2004 ; 203 : 127-37. crossref(new window)

Ishikura H, Nakamura Y, Kawano Y, Tanaka J, Mizunuma M, Ohta D, Nishida T, Murai A. Intravenous immunoglobulin improves sepsis-induced coagulopathy: A retrospective, single-center observational study. J Crit Care. 2015 ; 30(3) : 579-83. crossref(new window)

van de Vyver M, Engelbrecht L, Smith C, Myburgh KH. Neutrophil and monocyte responses to downhill running: Intracellular contents of MPO, IL-6, IL-10, pstat3, and SOCS3. Scand J Med Sci Sports. 2015 ; doi: 10.1111/sms.12497. crossref(new window)

Jones SA, Scheller J, Rose-John S. Therapeutic strategies for the clinical blockade of IL-6/gp130 signaling. J Clin Invest. 2011 ; 121(9) : 3375-83. crossref(new window)

Han JM, Jin YY, Kim HY, Park KH, Lee WS, Jeong TS. Lavandulyl flavonoids from Sophora flavescens suppress lipopolysaccharide-induced activation of nuclear factor-kappaB and mitogen- activated protein kinases in RAW264.7 cells. Biol Pharm Bull. 2010 ; 33(6) : 1019-23. crossref(new window)

Su YW, Chiou WF, Chao SH, Lee MH, Chen CC, Tsai YC. Ligustilide prevents LPS-induced iNOS expression in RAW 264.7 macrophages by preventing ROS production and down-regulating the MAPK, NF-κB and AP-1 signaling pathways. Int Immunopharmacol. 2011 ; 11(9) : 1166-72. crossref(new window)