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Prevention effect of Allium tuberosum extract on ateriosclerosis in LDL Receptor Knockout Mouse
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  • Journal title : The Korea Journal of Herbology
  • Volume 31, Issue 2,  2016, pp.13-19
  • Publisher : The Korea Association of Herbology
  • DOI : 10.6116/kjh.2016.31.2.13.
 Title & Authors
Prevention effect of Allium tuberosum extract on ateriosclerosis in LDL Receptor Knockout Mouse
Kwon, OJun; Lee, JooYoung; Roh, Seong-Soo;
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Objectives : The present study was designed to evaluate the protective effect of Allium tuberosum (AT) extract on atherosclerosis in LDL receptor knockout (LDLr KO) mouse fed western diet.Methods : The AT was extracted 70% ethanol. The experimental groups were divided with four groups of LDLr KO mice, one group fed a normal diet and the others fed a Western diets for 8weeks. Two Western diet groups were orally administered AT extract at dosage of 100 and 300 mg/kg body weight. The body weight and food intake were measured every day. We measured levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and Glucose in serum. Also, effect of AT extract performed using H&E staining.Results : The AT treatment groups showed decrease in body weight and food efficiency in comparison with control group. Blood biochemistry parameters such as TG, TC, LDL, and glucose levels were increased in control group, while AT treatment groups were reduced. Also, the increased levels of ALT and AST were improved by AT extract. We confirmed that the weights of liver, kidney, subcutaneous fat, epididymal fat, kidney leaf fat, and intraabdominal fat were change in LDLr KO mice treated AT extract. In addition, histopathological changes in liver and aorta were similar to normal group.Conclusions : Based on these results, the AT extract is considered to make prevention of atherosclerosis through reduction and functional improvement of the liver and vascular endothelial cells in the body fat accumulation and lipid content in LDLr ko mouse model.
Allium tuberosum;atherosclerosis;LDL receptor knockout mouse;
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Kim the Korea national health and nutrition examination survey (KNHANES): current status and challenges. Epidemiol Health. 2014 ; 36 : 1-3.

Kannel WB, Castelli WP, Gordon T, McNamara PM. Serum cholesterol, lipoproteins and the risk of coronary heart disease. The Framingham study. Ann Intern Med. 1971 ; 74 : 1. crossref(new window)

Nigel Mackman. Triggers, targets and treatments for thrombosis. Nature. 2008 ; 451 : 914-8. crossref(new window)

Ryu SH. Antioxidative effects of kimchi and kimchi ingredients in vitro and in vivo systems. Inje Univ. 1997.

Wenzel U, Kuntz S, Brendel MD, Daniel H. Dietary flavone is a potent apoptosis inducer in human colon carcinoma cells. Cancer Res. 2000 ; 60 : 3823-31.

Ahn JM, Lee SH, Song YS. Biological functions in leek. Food Ind Nutr. 2001 ; 6 : 68-73.

Lim TS, Oh HI, Do JR, Kim HK. Physiological Actives of Leek Extracts from Allium tuberosum and Allium senescenes. J Kor Soc Food Sci Nutr. 2006 ; 35(3) : 301-6. crossref(new window)

Do JR, Kang SN, Kim KJ, Jo JH, Lee SW. Antimicrobial and Antioxidant Activities and Phenolic Contents in the Water Extract of Medicinal Plants. Food Sci Biotechnol. 2004 ; 13(5) : 640-5.

Chung HD, Youn SJ. The Physioecological Characteristics and Productivity of the Korean Native Allium tuberosum Rottl. J Kor Soc Hort Sci. 1996 ; 37(4) : 495-504.

Cha JY, Kim SK, Kim HJ, Song JY, Cho YS. Chemical Compositions and Antioxidative Activity of Leek (Allium turberusum) Seeds. Kor J Life Sci. 2000 ; 10(3) : 273-8.

Park YC, Lee JS, Kim MH, Kim DY, Lee SD. Pharmacological action and Toxicity of Angelica sinensis. Korean J Orient Med Pre. 2011 ; 19(2) : 93-108.

Gesina L. Longenecker. The Platelet: Phys iology and Pharmacology. Academic Press INC. 1985 : 17.

Kumar V, Abbas AK. Robbins Basic Pathology. Elsevier INC. 8th ed. 2007 : 10.

Srivastava RA, Mistry S, Sharma S. A novel antiinflammatory natural product from Sphaeranthus indicus inhibits expression of VCAM1 and ICAM1, and slows atherosclerosis progression independent of lipid changes. Nutr Metab (Lond). 2015 ; 12 : 20. crossref(new window)

Gargalovic PS. Atherogenesis on the Chopping Block. Cell Metab. 2009 ; 9(5) : 399-401. crossref(new window)

Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL. Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice. Circulation. 2008 ; 117(3) : 411-20. crossref(new window)

Ko KW, Paul A, Ma K, Li L, Chan L. Endothelial lipase modulates HDL but has no effect on atherosclerosis development in apoE-/- and LDLR-/- mice. J Lipid Res. 2005 ; 46(12) : 2586-94. crossref(new window)

Whitman SC. A practical approach to using mice in atherosclerosis research. Clin Biochem Rev. 2004 ; 25(1) : 81-93.

Breslow JL. Mouse models of atherosclerosis. Science. 1996 ; 272(5262) : 685-8. crossref(new window)

Ham IH, Lee UC, Lee BH, Choi HY. Lipid lowering activity of Ponciri Fructus and Aurantii Fructus Immaturus on hyperlipemia rats induced by Triton WR-1339. Korea J Herbol. 2007 ; 22(3) : 109-16.

Kang SS, Shin YJ. Study of the Effects of Samulhwalhyeol- tang in Hyperlipidemic Animal Model Induced with a High-Fat Diet. J Korean Orient Int Med. 2014 ; 35(2) : 119-32.

Lim AK, Jung MJ, Kim DW, Hong JH, Jung HK, Kim KS, Kim YH, Kim DI. An Extrapolation Concentration Decision Effect Antihyperlipidemic of Aglycone Isoflavone from Biotransformation Soybean on the Fed High-Fat Diet Rats. J Korean Soc Food Sci Nutr. 2009 ; 38(9) : 1167-73. crossref(new window)