Advanced SearchSearch Tips
Anti-obese Function of Polysaccharides derived from Korean Ginseng (Panax ginseng C.A. Meyer) and Development of Functional Food Material in Preventing Obesity
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : The Korea Journal of Herbology
  • Volume 31, Issue 4,  2016, pp.71-77
  • Publisher : The Korea Association of Herbology
  • DOI : 10.6116/kjh.2016.31.4.71.
 Title & Authors
Anti-obese Function of Polysaccharides derived from Korean Ginseng (Panax ginseng C.A. Meyer) and Development of Functional Food Material in Preventing Obesity
Shon, Myung-Soo; Kim, Gyo-Nam;
  PDF(new window)
Objectives : Adipogenesis was defined as a differentiation process of preadipocytes into the adipocytes. Thus, to control of this process can be one of the most important strategies to prevent obesity. Korean ginseng(Panax ginseng C.A. Meyer) is one of the most widely used medicinal herbs. Although multiple biological activities of Korean ginseng, particularly ginsenosides, have been known, the anti-adipogenic role and function of polysaccharides from Korean ginseng are still unclear. In this study, we examined anti-adipogenic activity of polysaccharides and its molecular basis mechanisms are further investigated.Methods : The cytotoxicity of KGP in 3T3-L1 was evaluated by MTT assay. Anti-adipogenic effect of KGP was examined by Oil Red O (ORO) staining and microscopy observation in 3T3-L1 mature adipocytes. The mRNA expression levels of adipogenic transcriptional factors were analyzed by reverse transcription-polymer chain reaction (RT-PCR). To elucidate the adipogenic molecular mechanism of KGT, SB431542 (TGF-β specific inhibitor) was used.Results : We found that polysaccharides showed no effect on the viability of 3T3-L1 preadipocytes. Dose dependent inhibitory effect of polysaccharides on 3T3-L1 adipogenesis was observed as judged by ORO staining and microscopic image analysis. To obtain further mechanistic insight into anti-adipogenic function of polysaccharides, we then tested the effect of polysaccharides treatment on the adipogenic marker genes. The mRNA expressions level of C/EBPα, PPARγ, C/EBPβ, and fatty acid synthase (FAS) were dose-dependently inhibited by KGP treatment in 3T3-L1 mature adipocytes.Conclusions : In conclusion, these findings suggest that the KGP could be used in treatment of obesity and overweight related diseases.
Korean ginseng;adipogenesis;obesity;polysaccharides;3T3-L1;
 Cited by
Curhan GC, Willett WC, Rimm EB, Spiegelman D, Ascherio AL, Stampfer MJ. Birth weight and adult hypertension, diabetes mellitus, and obesity in US men. Circulation. 1996 ; 94 : 3246-50. crossref(new window)

Gregoire FM, Smas CM, Sul HS. Understanding adipocyte differentiation. Physiol Rev. 1998 ; 78 : 783-809.

Spalding KL, Arner E, Westermark PO, Bernard S, Buchholz BA, Bergmann O, Blomqvist L, Hoffstedt J, Naslund E, Britton T, Concha H, Hassan M, Ryden M, Frisen J, Arner P. Dynamics of fat cell turnover in humans. Nature. 2008 ; 453 : 783-7. crossref(new window)

James AW. Review of signaling pathways governing MSC osteogenic and adipogenic differentiation. Scientifica. 2013 ; 17 : Article ID 684736.

Smas CM, Sul HS. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell. 1993 ; 73 : 725-34. crossref(new window)

Ross SE, Hemati N, Longo KA, Bennett CN, Lucas PC, Erickson RL, MacDougald OA. Inhibition of adipogenesis by Wnt signaling. Science. 2000 ; 289 : 950-3. crossref(new window)

Choy L, Skillington J, Derynck R. Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. J Cell Biol. 2000 ; 149 : 667-82. crossref(new window)

Kim CY, Kim GN, Wiacek JL, Chen CY, Kim KH. Selenate inhibits adipogenesis through induction of transforming growth factor-β1 (TGF-β1) signaling. Biochem Bioph Res Co. 2012 ; 426 : 551-7. crossref(new window)

Oh CH, Kim GN, Lee SH, Lee JS, Jang HD. Effects of heat processing time on total phenolic content and antioxidant capacity of ginseng Jung Kwa. J Ginseng Res. 2010 ; 34 : 198-204. crossref(new window)

Lim JK, Kang HJ, Kang SN, Lee BY. Antioxidant and antimicrobial activities of various solvent fractions of fine ginseng root. Food Sci Biotechnol. 2009 ; 18 : 513-8.

Tchilian EZ, Zhelezarov IE, Hadjiivanova CI. Effect of ginsenoside Rg1 on insulin binding in mice liver and brain membranes. Phytother Res. 1991 ; 5 : 46-8. crossref(new window)

Bray GA. Pathophysiology of obesity. Am J Clin Nutr. 1992 ; 55(suppl) : 488-94.

Li XL, Wang CZ, Mehendale SR, Sun S, Wang Q, Yuan CS. Panaxadiol, a purified ginseng component, enhances the anti-cancer effects of 5-fluorouracil in human colorectal cancer cells. Cancer Chemother Pharmacol. 2009 ; 64 : 1097-104. crossref(new window)

Lee KY, Park JA, Chung E, Lee YH, Kim SI, Lee SK. Ginsenoside-Rh2 blocks the cell cycle of SK-HEP-1 cells at the G1/S boundary by selectively inducing the protein expression of p27kip1. Cancer Lett. 1996 ; 110 : 193-200. crossref(new window)

Jin YH, Yim H, Park JH, Lee SK. Cdk2 activity is associated with depolarization of mitochondrial membrane potential during apoptosis. Biochem Biophys Res Commun. 2003 ; 305 : 974-80. crossref(new window)

Oh GS, Pae HO, Choi BM, Seo EA, Kim DH, Shin MK. 20(S)-Protopanaxatriol, one of ginsenoside metabolites, inhibits inducible nitric oxide synthase and cyclooxygenase-2 expressions through inactivation of nuclear factor-kappa B in RAW 264.7 macrophages stimulated with lipopolysaccharide. Cancer Lett. 2004 ; 205 : 23-9. crossref(new window)

Ntambi JM, Kim YC. Adipocyte differentiation and gene expression. J Nutr. 2000 ; 130(12) : 3122S-26S.

Park HS, Shim SM, Kim GH. Inhibitory effects of ethyl acetate-soluble fraction from morus alba on lipid accumulation in 3T3-L1 cells. Nat Prod Commun. 2013 ; 8(11) : 1579-82.

Kim CY, Le TT, Chen C, Cheng JX, Kim KH. Curcumin inhibits adipocyte differentiation through modulation of mitotic clonal expansion. J Nutr Biochem. 2011 ; 22(10) : 910-20. crossref(new window)

Bhatia V, Viswanathan P. Insulin resistance and PPAR insulin sensitizers. Curr Opin Investig Drugs. 2006 ; 7(10) : 891-7.

Harp JB. New insights into inhibitors of adipogenesis. A Curr Opin Lipidol. 2004 ; 15 : 303-7. crossref(new window)

Shi Y, Massague J. Mechanisms of TGF-beta signaling from cell membrane to the nucleus. Cell. 2003 ; 113 : 685-700. crossref(new window)

Wharton K, Derynck R. TGFβ family signaling: novel insights in development and disease, Development. 2009 ; 136 : 3691-7. crossref(new window)