In this study, free vibration analysis of double through-the-width split beam is studied based on the author's earlier work. Each segment which constructs double through-the-width split beam is considered as Timoshenko beam. The effect of coupling between longitudinal and transverse vibration on the natural frequencies of split beams is considered. Data acquisition and modal test of double split beam for clamped-free boundary condition are carried out. Experimental and numerical results obtained by ANSYS were compared with the calculated data by present theory and their comparisons give good agreement with one another. The influences of the size and location of double split, shear deformation, and boundary conditions on the natural frequencies are demonstrated for illustrative purpose. Effects of double split on the dynamic characteristics of beams can be used to detect the size and the location of damages in structures.