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A 4-week Repeated dose Oral Toxicity Study of Mecasin in Sprague-Dawley Rats to Determine the Appropriate Doses for a 13-week, Repeated Toxicity Test
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  • Journal title : Journal of Pharmacopuncture
  • Volume 18, Issue 4,  2015, pp.45-50
  • Publisher : KOREAN PHARMACOPUNCTURE INSTITUTE
  • DOI : 10.3831/KPI.2015.18.037
 Title & Authors
A 4-week Repeated dose Oral Toxicity Study of Mecasin in Sprague-Dawley Rats to Determine the Appropriate Doses for a 13-week, Repeated Toxicity Test
Cha, Eunhye; Lee, Jongchul; Lee, Seongjin; Park, Manyong; Song, Inja; Son, Ilhong; Song, Bong-Keun; Kim, Dongwoung; Lee, Jongdeok; Kim, Sungchul;
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 Abstract
Objectives: In this study, we investigated the 4-week repeated-dose oral toxicity of gami-jakyak gamcho buja decoction (Mecasin) to develop safe treatments. Methods: In order to investigate the 4-week oral toxicity of Mecasin, we administered Mecasin orally to rats. Sprague-Dawley (SD) rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of Mecasin of 500, 1,000, and 2,000 mg/kg of body weight were administered to the experimental groups, and a dose of normal saline solution of 10 mL/kg was administered to the control group. We examined the survival rate, weight, clinical signs, and gross findings for four weeks. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: No deaths occurred in any of the four groups. No significant changes in weights or food consumption between the control group and the experimental groups were observed. Serum biochemistry revealed that some groups showed significant decrease in inorganic phosphorus (IP) (P < 0.05). During necropsy on the rats, one abnormal macroscopic feature, a slight loss of fur, was observed in the mid dosage (1,000 mg/kg) male group. No abnormalities were observed in any other rats. In histopathological findings, the tubular basophilia and cast of the kidney and extramedullary hematopoiesis of the spleen were found. However, those changes were minimal and had occurred naturally or sporadically. No other organ abnormalities were observed. Conclusion: During this 4-week, repeated, oral toxicity test of Mecasin in SD rats, no toxicity changes due to Mecasin were observed in any of the male or the female rats in the high dosage group. Thus, we suggest that the doses in a 13-week, repeated test should be 0, 500, 1,000, and 2,000 mg/kg respectively.
 Keywords
four week repeated toxicity test;gami jakyak gamcho buja decoction (mecasin);herbal medicine;sprague-dawley rat;
 Language
English
 Cited by
 References
1.
Guo L, Cho SY, Kang SS, Lee SH, Baek HY, Kim YS. Orthogonal array design for optimizing extraction efficiency of active constituents from jakyak-gamcho decoction, the complex formula of herbal medicines, Paeoniae radix and Glycyrrhizae radix. J Ethnopharmacol. 2007;113(2):306-11. crossref(new window)

2.
Kang TH, Baek HY, Kim YC. Protective effect of jakyak- gamcho-tang extract and its constituents against t-BHP-induced oxidative damage in HT22 cells. Am J Chin Med. 2005;33(2):181-9. crossref(new window)

3.
Lee JM, Hong SY, Oh MS. [Effects of jakyakkamchobuja- tang on papain-induced osteoarthritis in mice]. J Korean Oriental Med. 2013;34(1):116-35. Korean.

4.
Jeong HH, Lee JC, Cha EH, Park MY, Son IH, Song BK, et al. A study on the oral toxicity of mecasin in rats. J Pharmacopuncture. 2014;17(4):61-5. crossref(new window)

5.
Elinav E, Chajek-Shaul T. Licorice consumption causing severe hypokalemic paralysis. Mayo Clin Proc. 2003;78(6):767-8. crossref(new window)

6.
Heo JI, Kim JH, Lee JM, Kim SC, Park JB, Kim JB, et al. Antioxidant activity and its mechanism of Paeonia lactiflora pall extract. Nat Prod Sci. 2013;19(1):49-53.

7.
Lee HD, Song CH. [Effect of Glycyrrhizae radix pharmacopuncture on PCA and secretion of ${\beta}$-hexosaminidase and cytokines in RBL-2H3 cells]. Korean J Acupunct. 2011;28(3):77-83. Korean.

8.
Park HJ, Shim HS, Kim H, Kim KS, Lee H, Hahm DH, Shim I. Effects of Glycyrrhizae radix on repeated restraint stress-induced neurochemical and behavioral responses. Korean J Physiol Pharmacol. 2010;14(6):371-6. crossref(new window)

9.
Tong P, Wu C, Wang X, Hu H, Jin H, Li C. et al. Development and assessment of a complete-detoxi arthritis cation strategy for fuzi (lateral root of aconitum carmichaeli) and its application in rheumatoid therapy. J Ethnopharmacol. 2013;146(2):562-71. crossref(new window)

10.
Singhuber J, Zhu M, Prinz S, Kopp B. Aconitum in traditional Chinese medicine: a valuable drug or an unpredictable risk?. J Ethnopharmacol. 2009;126(1):18-30. crossref(new window)

11.
Kim JK, Kim SH, Lee SM, Jeong HH, Park MY, Kim DW, et al. Study of single-dose toxicity of Aconitum kusnezoffii reichb pharmacopuncture in rats. J Pharmacopuncture. 2012;15(3):48-52. crossref(new window)

12.
Jeong SH, Park DS. [The analgesic and anti-imflamatory effect of A.I.P.R aqua-acupuncture on the arthritis induced by freund's complete adjuvant in rats]. The Acupuncture. 1997;14(1):334-46. Korean.

13.
Park CH, Kim YS. [Effects of salviae Miltiorrhizae radix on blood-brain barrier impairment of ICH-induced rats]. Korea J Herbol. 2014;29(1):19-26. Korean.

14.
Lee HG, Kim YS. [Effects of Salvia miltriorrhiza radix on neuronal apoptosis following intracerebral hemorrhage of rats]. Korea J Herbol. 2012;27(3):89-94. Korean.

15.
Yang CH, Kwen YZ, Kim MR. Effect of Gastrodiae rhizoma extract on blood pressure and plasma catecholamine level in unanaesthetized spontaneousely hypertensive rats. J Korean Orient Med. 1995;16(2):433-46.

16.
Kim WC, Jeong JK, Kim JS, Kim KO. [The verify of memory improvement by Gastrodia elata blume]. J of Oriental Neuropsychiatry. 2013;24(1):27-44. Korean. crossref(new window)

17.
Oh HS, Kim BW. [Anti-inflammatory activity of the water extract of Polygala tenuifolia willd]. J Korean Orient Intern Med. 2013;34(2):204-14. Korean.

18.
Hwang JY, Wu YX, Hwang DI, Bae SJ, Kim TW. [Anti- obesity effect of Polygala tenuifolia]. Korean J Food Preserv. 2014;21(1):97-106. Korean. crossref(new window)

19.
Shin IJ, Son SU, Park HW, Kim YR, Park SH, Swanberg K, et al. Preclinical evidence of rapid-onset antidepressant- like effect in Radix polygalae extract. PLoS One. 2014;9(2):e88617. crossref(new window)

20.
Cho SI. [Curcuma longa L. extract controls cancer cell (sarcoma 180) growth]. Korea J Herbol. 2005;20(4):27-31. Korean.

21.
Oh HI, Park HB, Ju MS, Jung SY, Oh MS. [Comparative study of anti-oxidant and anti-inflammatory activities between Curcumae longae radix and Curcumae longae rhizoma]. Korea J Herbol. 2010;25(1);83-91. Korean.

22.
Choi YS, Han HS, Lee YJ. [Study of the intestinal motility effects of Chaenomelis sinensis fructus and Chaenomelis lagenariae fructus]. Korea J Herbol. 2010;25(4):113-20. Korean.

23.
Ryu HW, Kim YS, Lim EM. [The anti-inflammatory effects of Chaenomelis fructus herba water extract on mouse RAW 264.7 Cell]. J Orient Obstet Gynecol. 2012;25(3):1-15. Korean.

24.
Kim YK. [The principle and test method toxicology]. Seoul: Donghwagisul; 1994(3). p. 15-8. Korean.

25.
Kim YG. [Toxicology]. Seoul: Donghwagisul; 1984. p. 15-8. Korean.

26.
Korea Food & Drug Administration. Korea Food & Drug Administration notification. Seoul: Korea Food & Drug Administration; 2005. p. 60.