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Molecular Characterization of Fluoroquinolone Resistant Escherichia coli Isolates from Chickens in Korea
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  • Journal title : Journal of Digital Convergence
  • Volume 14, Issue 4,  2016, pp.371-378
  • Publisher : The Society of Digital Policy and Management
  • DOI : 10.14400/JDC.2016.14.4.371
 Title & Authors
Molecular Characterization of Fluoroquinolone Resistant Escherichia coli Isolates from Chickens in Korea
Sung, Ji-Youn; Oh, Ji-Eun;
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 Abstract
An aim of current study was to investigate the prevalence and the mechanism of quinolone-resistance in E. coli isolates obtained from chicken cecum in Korea. In addition, multilocus sequence typing (MLST) was also performed for the molecular characterization of E. coli isolates. In an antimicrobial susceptibility test by the disk diffusion method, the 63.5% (54/85) of E. coli isolates showed the resistance to quinolone group of antimicrobial agents. All of the 54 E. coli isolates showing resistant to quinolone group had sense mutations in gyrA gene and point mutations at the , , or residues in parC gene were detected in 90.7% of the isolates. Interestingly, E. coli ST was closely related to amino acid substitutions in parE gene. Our results indicated that the long-term use of antimicrobial agents in food-producing animals was strongly associated with a prevalence of antimicrobial resistance in commensal Enterobacteriaceae, suggesting the need for continuous surveillance and monitoring of antimicrobial resistant determinants in bacterial isolates from food animals.
 Keywords
Antimicrobial resistance;Chicken;E. coli;Food animals;Multilocus sequence typing;Quinolones;
 Language
English
 Cited by
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