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Molecular Characterization of Fluoroquinolone Resistant Escherichia coli Isolates from Chickens in Korea
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  • Journal title : Journal of Digital Convergence
  • Volume 14, Issue 4,  2016, pp.371-378
  • Publisher : The Society of Digital Policy and Management
  • DOI : 10.14400/JDC.2016.14.4.371
 Title & Authors
Molecular Characterization of Fluoroquinolone Resistant Escherichia coli Isolates from Chickens in Korea
Sung, Ji-Youn; Oh, Ji-Eun;
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An aim of current study was to investigate the prevalence and the mechanism of quinolone-resistance in E. coli isolates obtained from chicken cecum in Korea. In addition, multilocus sequence typing (MLST) was also performed for the molecular characterization of E. coli isolates. In an antimicrobial susceptibility test by the disk diffusion method, the 63.5% (54/85) of E. coli isolates showed the resistance to quinolone group of antimicrobial agents. All of the 54 E. coli isolates showing resistant to quinolone group had sense mutations in gyrA gene and point mutations at the , , or residues in parC gene were detected in 90.7% of the isolates. Interestingly, E. coli ST was closely related to amino acid substitutions in parE gene. Our results indicated that the long-term use of antimicrobial agents in food-producing animals was strongly associated with a prevalence of antimicrobial resistance in commensal Enterobacteriaceae, suggesting the need for continuous surveillance and monitoring of antimicrobial resistant determinants in bacterial isolates from food animals.
Antimicrobial resistance;Chicken;E. coli;Food animals;Multilocus sequence typing;Quinolones;
 Cited by
N. Aoike, T. Saga, R. Sakata, A. Yoshizumi, S. Kimura, M. Iwata, S. Yoshizawa, Y. Sugasawa, Y. Ishii, K. Yamaguchi, K. Tateda, "Molecular characterization of extraintestinal Escherichia coli isolates in Japan: relationship between sequence types and mutation patterns of quinolone resistance-determining regions analyzed by pyrosequencing", J. Clin. Microbiol., Vol. 51, pp. 1692-1698, 2013. crossref(new window)

V. Cattoir, L. Poirel, V. Rotimi, CJ. Soussy, P. Nordmann, "Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates", J. Antimicrob. Chemother, Vol. 60, pp. 394-397, 2007. crossref(new window)

"Clinical and Laboratory Standards Institute (CLSI). Performance standard for antimicrobial susceptibility testing; Twenty first informational supplement CLSI Document". M100-S21, Vol. 31, No. 1. Clinical and Laboratory Standards nstitute, Wayne, PA, 2010.

H. K. Dessie, D. H. Bae, Y. J. Lee, "Characterization of integrons and their cassettes n Escherichia coli and Salmonella isolates from oultry in Korea", Poult Sci., Vol. 92, pp. 036-3043, 2013.

P. Heisig, "Genetic evidence for a role of parC mutations in development of high-level fluoroquinolone resistance in Escherichia coli, Antimicrob". Agents Chemother., Vol. 40, pp. 879-885, 1996.

D. C. Hooper, "Mechanisms of action and resistance of older and newer fluoroquinolones". Clin. Infect. Dis., Vol. 31, Suppl. pp. 24-28, 2000. crossref(new window)

E. K. Hyytia-Trees, K. Cooper, E.M. Ribot, P. Gerner-Smidt, "Recent developments and future prospects in subtyping of food borne bacterial pathogens", Future Microbiol., Vol. 2, pp. 175-185, 2007. crossref(new window)

L. B. Jensen, F. J. Angulo, K. Molbak, H. C. Wegener, "Human health risks associated with antimicrobial use in animals, In Guardabassi L, Jensen LB, Kruse H, editores". p.13-26. Guide to Antimicrobial Use in Animals. Blackwell Publishing Ltd, Oxford, UK, 2008.

H. Y. Kang, Y. S. Jeong, J. Y. Oh, S. H. Tae, C. H. Choi, D. C. Moon, W. K. Lee, Y.C Lee, S. Y. Seol, D. T. Cho, J. C. Lee, "Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea", J. Antimicrob. Chemother., Vol. 55, pp. 639-644, 2005. crossref(new window)

M. Karczmarczyk, M. Martins, T. Quinn, N. Leonard, S. Fanning, "Mechanisms of fluoroquinolone resistance in Escherichia coli isolates from food-producing animals", Appl. Environ. Microbiol., Vol. 77, pp. 7113-7120, 2011. crossref(new window)

S. Paltansing, M. E. Kraakman, J. M. Ras, E. Wessels, A. T. Bernards, "Characterization of fluoroquinolone and cephalosporin resistance mechanisms in Enterobacteriaceae isolated in a Dutch teaching hospital reveals the presence of an Escherichia coli ST131 clone with a specific mutation in parE", J. Antimicrob. Chemother., Vol. 68, pp. 40-45, 2013. crossref(new window)

I. Phillips, M. Casewell, T. Cox, B. De Groot, C. Friis, R. Jones, C. Nightingale, R. Preston, J. Waddell, "Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data", J. Antimicrob. Chemother., Vol. 53, pp. 28-52, 2004.

B. W. Shaheen, R. Nayak, S. L. Foley, D. M. Boothe, "Chromosomal and plasmid-mediated fluoroquinolone resistance mechanisms among broad-spectrum-cephalosporin-resistant Escherichia coli isolates recovered from companion animals in the USA", J. Antimicrob. Chemother., Vol. 68, pp. 1019-1024, 2013. crossref(new window)

J. Strahilevitz, G. A. Jacoby, D. C. Hooper, A. Robicsek, "Plasmid mediated quinolone resistance: A multifaceted threat", Clin. Microbiol. Rev., Vol. 22, pp. 664-689, 2009. crossref(new window)

M. D. Tamang, H. M. Nam, M. H. Chae, S. R. Kim, M. Gurung, G. C. Jang, S. C. Jung, S. K. Lim, "Prevalence of plasmid-mediated quinolone resistance determinants among Escherichia coli isolated from food animals in Korea". Foodborne Pathog. Dis., Vol. 9, pp. 1057-1063, 2012. crossref(new window)

C. Torres, M. Zarazaga, "Antibiotics as growth promoters in animals. Are we going down the right road". Gac. Sanit., Vol. 16, pp. 109-112, 2002. crossref(new window)

T. Wirth, D. Falush, R. Lan, F. Colles, P. Mensa, L. H. Wieler, H. Karch, P. R. Reeves, M. C. Maiden, H. Ochman, M. Achtman, "Sex and virulence in Escherichia coli: an evolutionary perspective", Mol. Microbiol., Vol. 60, pp. 1136-1151, 2006. crossref(new window)

H. Y. Yang, Y.S. Nam, H. J. Lee, "Prevalence of plasmid-mediated quinolone resistance genes among ciprofloxacin-nonsusceptible Escherichia coli and Klebsiella pneumoniae isolated from blood cultures in Korea", Can. J. Infect. Dis. Med. Microbiol., Vol. 25, pp. 163-169, 2014.

Sanghyuk Lee, "Grouping DNA sequences with similarity measure and application", Journal of the Korea Convergence Society, Vol. 4, No. 3, pp. 35-41, 2013.

Jae-Il Han, Hyun-Ho Sung, Chang-Eun Park, "Study on Convergence Technique Using the Antimicrobial Resistance and Virulence Genes Analysis in Escherichia coli", Journal of the Korea Convergence Society, Vol. 6, No. 5, pp. 77-84, 2015.