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Effects of Styrene-metabolizing Enzyme Polymorphisms and Lifestyle Behaviors on Blood Styrene and Urinary Metabolite Levels in Workers Chronically Exposed to Styrene
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  • Journal title : Toxicological Research
  • Volume 31, Issue 4,  2015, pp.355-361
  • Publisher : The Korean Society of Toxicology
  • DOI : 10.5487/TR.2015.31.4.355
 Title & Authors
Effects of Styrene-metabolizing Enzyme Polymorphisms and Lifestyle Behaviors on Blood Styrene and Urinary Metabolite Levels in Workers Chronically Exposed to Styrene
Kim, Ki-Woong;
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The aim of this study was to investigate whether genetic polymorphisms of CYP2E1, GSTM1, and GSTT1 and lifestyle habits (smoking, drinking, and exercise) modulate the levels of urinary styrene metabolites such as mandelic acid (MA) and phenylglyoxylic acid (PGA) after occupational exposure to styrene. We recruited 79 male workers who had received chronic exposure in styrene fiberglass-reinforced plastic manufacturing factories. We found that serum albumin was significantly correlated with blood styrene/ambient styrene (BS/AS), urinary styrene (US)/AS, and US/BS ratios as well as urinary metabolites, that total protein correlated with US/MA and US/PGA ratios, and that low density lipoprotein (LDL)-cholesterol significantly correlated with US/BS, US/MA, and US/PGA ratios. Multiple logistic regression analyses using styrene-metabolizing enzyme genotypes and lifestyle habits as dependent variables and blood and urine styrene concentrations and urine styrene metabolite levels as independent variables revealed that was associated with the MA/US ratio and GSTM1 with US/BS, that a smoking habit was associated with US/AS and MA/US ratios and MA and PGA levels, and that regular exercise was correlated with PGA/US. In conclusion, the results suggested that genetic polymorphisms of styrene-metabolizing enzymes, lifestyle behaviors, and albumin and LDL-cholesterol serving as homeostasis factors together are involved in styrene metabolism.
Styrene;Metabolism;Gene polymorphisms;Biological monitoring;Workers;
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Toxicological Research, 2016. vol.32. 4, pp.337-343 crossref(new window)
Combined Toxic Effects of Polar and Nonpolar Chemicals on Human Hepatocytes (HepG2) Cells by Quantitative Property - Activity Relationship Modeling, Toxicological Research, 2016, 32, 4, 337  crossref(new windwow)
International Programme on Chemical Safety (IPCS). (1983) Styrene. Environmental Health Criteria 26. World Health Organization, Geneva, pp. 20-50.

Ministry of Employment and Labor (MoEL). (2013) 2013 Annual report for the health examination of workers. Ministry of Employment and Labor.

Watabe, T., Ozawa, N. and Yoshkawa, K. (1981) Stereochemistry in the oxidative metabolism of styrene by hepatic microsomes. Biochem. Pharmacol., 30, 1695-1698. crossref(new window)

Ohtsuji, H. and Ikeda, M. (1971) The metabolism of styrene in rat and the stimulatory effect of phenobarbital. Toxicol. Appl. Pharmacol., 18, 321-328. crossref(new window)

Laffon, B., Pasaro, E. and Mendez, J. (2001) Effects of styrene- 7,8-oxide over p53, p21, bcl-2 and bax expression in human lymphocyte cultures. Mutagenesis, 16, 127-132. crossref(new window)

Chakrabarti, S.K. (2000) Altered regulation of dopaminergic activity and impairment in motor function in rats after subchronic exposure to styrene. Pharmacol. Biochem. Behav., 66, 523-532. crossref(new window)

Husain, R., Srivastava, S.P., Mushtaq, M. and Seth, P.K. (1980) Effect of styrene on levels of serotonin, noradrenalin, dopamine and activity of acetyl cholinesterase and monoamine oxidase in rat brain. Toxicol. Lett., 7, 47-50. crossref(new window)

Sumner, S.J. and Fennell, T.R. (1994) Review of metabolic fate of styrene. Crit. Rev. Toxicol., 24, S11-33. crossref(new window)

American Conference of Government Industrial Hygienists (ACGIH). (2000) Threshold limit values (TLVs) for chemical substances and physical agents and biological exposure indices (BEIs). ACGIH, Cincinnati, OH.

Wenker, M.A., Kezic, S., Monster, A.C. and de Wolff, F.A. (2001) Metabolic capacity and interindividual variation in toxicokinetics of styrene in volunteers. Hum. Exp. Toxicol., 20, 221-228. crossref(new window)

De Palma, G., Manini, P., Mozzoni, P., Andreoli, R., Bergamaschi, E., Cavazzini, S., Franchini, I. and Mutti, A. (2001) Pholimorphism of xenobiotic-metabolizing enzymes and excretion of styrene-specific mercapturic acids. Chem. Res. Toxicol., 14, 1393-1400. crossref(new window)

Haufroid, V., Buchet, J.P., Gardinal, S., Ghittori, S., Imbriani, M., Hirvonen, A. and Lison, D. (2001) Importance of genetic polymorphism in interpreting drug-metabolizing enzymes for the interpretation of biomarkers of exposure of styrene. Biomarkers, 6, 236-249. crossref(new window)

Haufroid, V., Jakubowski, M., Janasik, B., Ligocka, D., Buchet, J.P., Bergamaschi, E., Manini, P., Mutti, A., Ghittori, S., Arand, M., Hangen, N., Oesch, F., Hirvonen, A. and Lison, D. (2002) Interest of genotyping and phenotyping of drugmetabolizing enzymes for the interpretation of biological monitoring of exposure to styrene. Pharmacogenetics, 12, 691-702. crossref(new window)

De Palma, G., Mozzoni, P., Scotti, E., Manini, P., Andreoli, R., Naccarati, A., Mercati, F., Migliore, L. and Mutti, A. (2003) [Genetic polymorphism of biotransforming enzymes and genotoxic effects of styrene]. G. Ital. Med. Lav. Ergon., 25 Suppl 3, 63-64.

Ma, M., Umemura, T., Mori, Y., Gong. Y., Saijo, Y., Sato, F., Kawai, T. and Kishi, R. (2005) Influence of genetic polymorphisms of styrene-metabolizing enzymes and smoking habits on levels of urinary metabolites after occupational exposure to styrene. Toxicol. Lett., 160, 84-91. crossref(new window)

Conccini, T., Di Nucci, A., Tonini, M., Maestri, L., Costa, L.G., Liuzzi, M. and Manzo, L. (1996) Effects of ethanol administration on cerebral non-protein sulfhydryl content in rats exposed to styrene vapour. Toxicology, 106, 115-122. crossref(new window)

NIOSH. (1996) NIOSH manual of analytical methods (4th edition). Method 1501, U.S. Department of Health and Human Services, Cincinnati.

Kim, K.W., Won, Y.L. and Ko, K.S. (2015) Ethnic differences in the metabolism of toluene: coparisons between Korean and foreign workers exposed to toluene. Toxicol. Res., 31, 25-32. crossref(new window)

de Carvalho, D., Lanchote, V.L., Bonato, P.S., Queiroz, R.H., Santos, A.C. and Dreossi, S.A. (1991) A new derivatization procedure for the analysis of hippuric acid and methyl hippuric acid by gaschromatography. Int. Arch. Occup. Environ. Health, 63, 33-37. crossref(new window)

Shaikh, R.S., Amir, M., Masood, A.I., Sohail, A., Athar, H.U., Siraj, S., Ali, M. and Anjam, M.S. (2010) Frequency distribution of GSTM1 and GSTT1 null allele in Parkistani population and risk of disease incidence. Environ. Toxicol. Pharmacol., 30, 76-79. crossref(new window)

Cai, L., Zheng, Z.L. and Zhang, Z.F. (2005) Cytochrome p4502E1 polymorphisms and the risk of gastric cardiac cancer. World J. Gastroenterol., 11, 1867-1871. crossref(new window)

Prieto-Castello, M.J., Cardona, A., Marhuenda, D., Roei, J.M. and Corno, A. (2010) Use of the CYP2E1 genotype and phenotype for the biological monitoring of occupational exposure to styrene. Toxicol. Lett., 192, 34-39. crossref(new window)

Kim, M.R. and Kim, C.W. (2007) Human blood plasma preparation for tow-dimensional gel electrophoresis. J. Chromatogr. B Anal. Technol. Biomed. Life Sci., 849, 203-210. crossref(new window)

Bern, M., Sand, K.M., Nilson, J., Sandlie, I. and Andersen, J.T. (2015) The role of albumin receptors in regulation of albumin homeostasis: Implications for drug delivery. J. Controlled Release, 211, 144-162. crossref(new window)

Carbonari, D., Mansi, A., Proietto, A.R., Paci, E., Bonanni, R.C., Gherardi, M., Gatto, M.P., Sisto, R. and Tranfo, G. (2015) Influence of genetic polymorphisms of styrene-metabolizing enzymes on the levels of urinary biomarkers of styrene exposure. Toxicol. Lett., 233, 156-162. crossref(new window)