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Antioxidant Activity and Anti-wrinkle Effects of Aceriphyllum rossii Leaf Ethanol Extract
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  • Journal title : Toxicological Research
  • Volume 31, Issue 4,  2015, pp.363-369
  • Publisher : The Korean Society of Toxicology
  • DOI : 10.5487/TR.2015.31.4.363
 Title & Authors
Antioxidant Activity and Anti-wrinkle Effects of Aceriphyllum rossii Leaf Ethanol Extract
Ha, Bi Gyeon; Park, Min Ah; Lee, Chae Myoung; Kim, Young Chul;
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We evaluated the antioxidant activity and anti-wrinkle effects of Aceriphyllum rossii leaf ethanol extract (ARLEE) in vitro using human dermal fibroblasts. The total polyphenol and flavonoid contents of ARLEE were 578.6 and 206.3 mg/g, respectively. At a concentration of , the electron-donating ability of ARLEE was 87.1%. In comparison with the vehicle, ARLEE treatment at significantly increased type I procollagen synthesis (p < 0.01) by 50.7%. In vitro ARLEE treatment (10 mg/mL) inhibited collagenase and elastase activity by 97.1% and 99.2%, respectively. Compared with the control, ascorbic acid treatment at significantly decreased matrix metalloproteinase (MMP)-1 protein expression (p < 0.01) by 37.0%. ARLEE treatment at significantly decreased MMP-1 protein expression (p < 0.01) by 46.1%. Ascorbic acid and ARLEE treatments at significantly decreased MMP-1 mRNA expression (p < 0.01) by 26.1% and 36.1%, respectively. From these results, we conclude that ARLEE has excellent antioxidant activity and even better anti-wrinkle effects than ascorbic acid in human dermal fibroblasts. These results suggest that ARLEE could be used in functional cosmetics for the prevention or alleviation of skin wrinkles induced by ultraviolet rays.
Aceriphyllum rossii;Antioxidant activity;Collagenase activity;MMP-1;Type I procollagen;
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Jenkins, G. (2002) Molecular mechanisms of skin aging. Mech. Ageing Dev., 123, 801-810. crossref(new window)

Berneburg, M., Plettenberg, H. and Krutmann, J. (2000) Photoaging of human skin. Photodermatol. Photoimmunol. Photomed., 16, 239-244. crossref(new window)

Di Lullo, G.A., Sweeney, S.M., Korkko, J., Ala-Kokko, L. and San Antonio, J.D. (2002) Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen. J. Biol. Chem., 277, 4223-4231. crossref(new window)

Chambers, A.F. and Matrisian, L.M. (1997) Changing views of the role of matrix metalloproteinases in metastasis. J. Natl. Cancer Inst., 89, 1260-1270. crossref(new window)

Welgus, H.G., Campbell, E.J., Cury, J.D., Eisen, A.Z., Senior, R.M., Wilhelm, S.M. and Goldberg, G.I. (1990) Neutral metalloproteinases produced by human mononuclear phagocytes. Enzyme profile, regulation, and expression during cellular development. J. Clin. Invest., 86, 1496-1502. crossref(new window)

Offord, E.A., Gautier, J.C., Avanti, O., Scaletta, C., Runge, F., Kramer, K. and Applegate, L.A. (2002) Photoprotective potential of lycopene, ${\beta}$-carotene, vitamin E, vitamin C and carnosic acid in UVA-irradiated human skin fibroblasts. Free Radical Biol. Med., 32, 1293-1303. crossref(new window)

Ivanov, V., Ivanova, S., Roomi, M.W., Kalinovsky, T., Niedzwiecki, A. and Rath, M. (2007) Extracellular matrix-mediated control of aortic smooth muscle cell growth and migration by a combination of ascorbic acid, lysine, proline, and catechins. J. Cardiovasc. Pharmacol., 50, 541-547. crossref(new window)

Choi, C.P., Kim, Y.I., Lee, J.W. and Lee, M.H. (2007) The effect of narrowband ultraviolet B on the expression of matrix metalloproteinase-1, transforming growth factor-beta1 and type I collagen in human skin fibroblasts. Clin. Exp. Dermatol., 32, 180-185. crossref(new window)

Chen, C.L., Liou, S.F., Chen, S.J. and Shih, M.F. (2011) Protective effects of chlorella-derived peptide on UVB-induced production of MMP-1 and degradation of procollagen genes in human skin fibroblasts. Regul. Toxicol. Pharmacol., 60, 112-119. crossref(new window)

Matrisian, L.M., Ganser, G.L., Kerr, L.D., Pelton, R.W. and Wood, L.D. (1992) Negative regulation of gene expression by TGF-beta. Mol. Reprod. Dev., 32, 111-120. crossref(new window)

Han, J.T., Kim, H.Y., Park, Y.D., Lee, Y.H., Lee, K.R., Kwon, B.M. and Baek, N.I. (2002) Aceriphyllic acid A, a new ACAT inhibitory triterpenoid, from Aceriphyllum rossii. Planta Med., 68, 558-561. crossref(new window)

Ahn, E.M., Han, J.T., Kwon, B.M., Kim, S.H. and Baek, N.I. (2008) Anti-cancer activity of flavonoids from Aceriphyllum rossii. J. Korean Soc. Appl. Biol. Chem., 51, 309-315. crossref(new window)

Lim, S.H., Kim, H.Y., Park, M.H., Park, Y.H., Ham, H.J., Lee, K.Y., Kim, K.H., Park, D.S. and Kim, S.M. (2010) Biological activities of solvent extracts from leaves of Aceriphyllum rossii. J. Korean Soc. Food Sci. Nutr., 39, 1739-1744. crossref(new window)

Middleton, E. Jr. and Drzewiecki, G. (1984) Flavonoid inhibition of human basophil histamine release stimulated by various agents. Biochem. Pharmacol., 33, 3333-3338. crossref(new window)

Folin, O. and Denis, W. (1912) On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem., 12, 239-243.

Davies, R., Massey, R.C. and McWeeny, D.J. (1980) The catalysis of the N-nitrosation of secondary amines by nitrosophenols. Food Chem., 6, 115-122. crossref(new window)

Blois, M.S. (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200. crossref(new window)

Son, E.D., Choi, G.H., Kim, H., Lee, B., Chang, I.S. and Hwang, J.S. (2007) Alpha-ketoglutarate stimulates procollagen production in cultured human dermal fibroblasts, and decreases UVB-induced wrinkle formation following topical application on the dorsal skin of hairless mice. Biol. Pharm. Bull., 30, 1395-1399. crossref(new window)

Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275.

Wondrak, G.T., Roberts, M.J., Cervantes-Laurean, D., Jacobson, M.K. and Jacobson, E.L. (2003) Proteins of the extracellular matrix are sensitizers of photo-oxidative stress in human skin cells. J. Invest. Dermatol., 121, 578-586. crossref(new window)

Bickers, D.R. and Athar, M. (2006) Oxidative stress in the pathogenesis of skin disease. J. Invest. Dermatol., 126, 2565-2575. crossref(new window)

Seyoum, A., Asres, K. and El-Fiky, F.K. (2006) Structure-radical scavenging activity relationships of flavonoids. Phytochemistry, 67, 2058-2070. crossref(new window)

Chang, H.C., Huang, G.J., Agrawal, D.C., Kuo, C.L., Wu, C.R. and Tsay, H.S. (2007) Antioxidant activities and polyphenol contents of six folk medicinal ferns used as "Gusuibu". Bot. Stud., 48, 397-406.

Kim, Y.C., Choi, S.Y. and Park, E.Y. (2015) Anti-melanogenic effects of black, green, and white tea extracts on immortalized melanocytes. J. Vet. Sci., 16, 135-143. crossref(new window)

Kondo, S. (2000) The roles of cytokines in photoaging. J. Dermatol. Sci., 23 Suppl 1, S30-36. crossref(new window)

Godeau, G. and Hornebeck, W. (1988) Morphometric analysis of the degradation of human skin elastic fibers by human leukocyte elastase (EC 3-4-21-37) and human skin fibroblast elastase (EC 3-4-24). Pathol. Biol., 36, 1133-1138.

Fisher, G.J., Kang, S., Varani, J., Bata-Csorgo, Z., Wan, Y., Datta, S. and Voorhees, J.J. (2002) Mechanisms of photoaging and chronological skin aging. Arch. Dermatol., 138, 1462-1470.