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The ETS Factor, ETV2: a Master Regulator for Vascular Endothelial Cell Development
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  • Journal title : Molecules and Cells
  • Volume 38, Issue 12,  2015, pp.1029-1036
  • Publisher : Korea Society for Molecular and Cellular Biology
  • DOI : 10.14348/molcells.2015.0331
 Title & Authors
The ETS Factor, ETV2: a Master Regulator for Vascular Endothelial Cell Development
Oh, Se-Yeong; Kim, Ju Young; Park, Changwon;
  PDF(new window)
 Abstract
Appropriate vessel development and its coordinated function is essential for proper embryogenesis and homeostasis in the adult. Defects in vessels cause birth defects and are an important etiology of diseases such as cardiovascular disease, tumor and diabetes retinopathy. The accumulative data indicate that ETV2, an ETS transcription factor, performs a potent and indispensable function in mediating vessel development. This review discusses the recent progress of the study of ETV2 with special focus on its regulatory mechanisms and cell fate determining role in developing mouse embryos as well as somatic cells.
 Keywords
cell reprogramming;endothelial cells;ETV2;transcription factors;
 Language
English
 Cited by
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ETV-2 activated proliferation of endothelial cells and attenuated acute hindlimb ischemia in mice, In Vitro Cellular & Developmental Biology - Animal, 2017, 53, 7, 616  crossref(new windwow)
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Etv2 as an essential regulator of mesodermal lineage development, Cardiovascular Research, 2017  crossref(new windwow)
3.
The Hemogenic Competence of Endothelial Progenitors Is Restricted by Runx1 Silencing during Embryonic Development, Cell Reports, 2016, 15, 10, 2185  crossref(new windwow)
 References
1.
Abedin, M.J., Nguyen, A., Jiang, N., Perry, C.E., Shelton, J.M., Watson, D.K., and Ferdous, A. (2014). Fli1 acts downstream of Etv2 to govern cell survival and vascular homeostasis via positive autoregulation. Circ. Res. 114, 1690-1699. crossref(new window)

2.
Bartel, F.O., Higuchi, T., and Spyropoulos, D.D. (2000). Mouse models in the study of the Ets family of transcription factors. Oncogene 19, 6443-6454. crossref(new window)

3.
Barton, K., Muthusamy, N., Fischer, C., Ting, C.N., Walunas, T.L., Lanier, L.L., and Leiden, J.M. (1998). The Ets-1 transcription factor is required for the development of natural killer cells in mice. Immunity 9, 555-563. crossref(new window)

4.
Behrens, A.N., Zierold, C., Shi, X., Ren, Y., Koyano-Nakagawa, N., Garry, D.J., and Martin, C.M. (2014). Sox7 is regulated by ETV2 during cardiovascular development. Stem Cells Dev. 23, 2004-2013. crossref(new window)

5.
Berman, H.M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T.N., Weissig, H., Shindyalov, I.N., and Bourne, P.E. (2000). The Protein Data Bank. Nucleic Acids Res. 28, 235-242. crossref(new window)

6.
Bertrand, J.Y., Chi, N.C., Santoso, B., Teng, S., Stainier, D.Y., and Traver, D. (2010). Haematopoietic stem cells derive directly from aortic endothelium during development. Nature 464, 108-111. crossref(new window)

7.
Boisset, J.C., van Cappellen, W., Andrieu-Soler, C., Galjart, N., Dzierzak, E., and Robin, C. (2010). In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium. Nature 464, 116-120. crossref(new window)

8.
Bondue, A., Lapouge, G., Paulissen, C., Semeraro, C., Iacovino, M., Kyba, M., and Blanpain, C. (2008). Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification. Cell Stem Cell 3, 69-84. crossref(new window)

9.
Brown, T.A., and McKnight, S.L. (1992). Specificities of proteinprotein and protein-DNA interaction of GABP alpha and two newly defined ets-related proteins. Genes Dev. 6, 2502-2512. crossref(new window)

10.
Caprioli, A., Koyano-Nakagawa, N., Iacovino, M., Shi, X., Ferdous, A., Harvey, R.P., Olson, E.N., Kyba, M., and Garry, D.J. (2011). Nkx2-5 represses Gata1 gene expression and modulates the cellular fate of cardiac progenitors during embryogenesis. Circulation 123, 1633-1641. crossref(new window)

11.
Carmeliet, P., and Jain, R.K. (2011). Molecular mechanisms and clinical applications of angiogenesis. Nature 473, 298-307. crossref(new window)

12.
Choi, K. (2002). The hemangioblast: a common progenitor of hematopoietic and endothelial cells. J. Hematother. Stem Cell Res. 11, 91-101. crossref(new window)

13.
Chung, Y.S., Zhang, W.J., Arentson, E., Kingsley, P.D., Palis, J., and Choi, K. (2002). Lineage analysis of the hemangioblast as defined by FLK1 and SCL expression. Development 129, 5511-5520. crossref(new window)

14.
Ciau-Uitz, A., Wang, L., Patient, R., and Liu, F. (2013). ETS transcription factors in hematopoietic stem cell development. Blood Cells Mol. Dis. 51, 248-255. crossref(new window)

15.
Cohen, D.E., and Melton, D. (2011). Turning straw into gold: directing cell fate for regenerative medicine. Nat. Rev. Genet. 12, 243-252. crossref(new window)

16.
Craig, M.P., Grajevskaja, V., Liao, H.K., Balciuniene, J., Ekker, S.C., Park, J.S., Essner, J.J., Balciunas, D., and Sumanas, S. (2015). Etv2 and fli1b function together as key regulators of vasculogenesis and angiogenesis. Arterioscler. Thromb. Vasc. Biol. 35, 865-876. crossref(new window)

17.
De Haro, L., and Janknecht, R. (2002). Functional analysis of the transcription factor ER71 and its activation of the matrix metalloproteinase-1 promoter. Nucleic Acids Res. 30, 2972-2979. crossref(new window)

18.
De Haro, L., and Janknecht, R. (2005). Cloning of the murine ER71 gene (Etsrp71) and initial characterization of its promoter. Genomics 85, 493-502. crossref(new window)

19.
De Val, S., Chi, N.C., Meadows, S.M., Minovitsky, S., Anderson, J.P., Harris, I.S., Ehlers, M.L., Agarwal, P., Visel, A., Xu, S.M., et al. (2008). Combinatorial regulation of endothelial gene expression by ets and forkhead transcription factors. Cell 135, 1053-1064. crossref(new window)

20.
Dejana, E., Taddei, A., and Randi, A.M. (2007). Foxs and Ets in the transcriptional regulation of endothelial cell differentiation and angiogenesis. Biochim. Biophys. ACTA 1775, 298-312.

21.
Drake, C.J., and Fleming, P.A. (2000). Vasculogenesis in the day 6.5 to 9.5 mouse embryo. Blood 95, 1671-1679.

22.
Ema, M., Faloon, P., Zhang, W.J., Hirashima, M., Reid, T., Stanford, W.L., Orkin, S., Choi, K., and Rossant, J. (2003). Combinatorial effects of Flk1 and Tal1 on vascular and hematopoietic development in the mouse. Genes Dev. 17, 380-393. crossref(new window)

23.
Ema, M., Takahashi, S., and Rossant, J. (2006). Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors. Blood 107, 111-117. crossref(new window)

24.
Faloon, P., Arentson, E., Kazarov, A., Deng, C.X., Porcher, C., Orkin, S., and Choi, K. (2000). Basic fibroblast growth factor positively regulates hematopoietic development. Development 127, 1931-1941.

25.
Ferdous, A., Caprioli, A., Iacovino, M., Martin, C.M., Morris, J., Richardson, J.A., Latif, S., Hammer, R.E., Harvey, R.P., Olson, E.N., et al. (2009). Nkx2-5 transactivates the Ets-related protein 71 gene and specifies an endothelial/endocardial fate in the developing embryo. Proc. Natl. Acad. Sci. USA 106, 814-819. crossref(new window)

26.
Findlay, V.J., LaRue, A.C., Turner, D.P., Watson, P.M., and Watson, D.K. (2013). Understanding the role of ETS-mediated gene regulation in complex biological processes. Adv. Cancer Res. 119, 1-61. crossref(new window)

27.
Flamme, I., Frolich, T., and Risau, W. (1997). Molecular mechanisms of vasculogenesis and embryonic angiogenesis. J. Cell. Physiol. 173, 206-210. crossref(new window)

28.
Frum, T., and Ralston, A. (2015). Cell signaling and transcription factors regulating cell fate during formation of the mouse blastocyst. Trends Genet. 31, 402-410. crossref(new window)

29.
Ginsberg, M., James, D., Ding, B.S., Nolan, D., Geng, F., Butler, J.M., Schachterle, W., Pulijaal, V.R., Mathew, S., Chasen, S.T., et al. (2012). Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFbeta suppression. Cell 151, 559-575. crossref(new window)

30.
Gurdon, J.B. (2006). From nuclear transfer to nuclear reprogramming: the reversal of cell differentiation. Ann. Rev. Cell Devel. Biol. 22, 1-22. crossref(new window)

31.
Haar, J.L., and Ackerman, G.A. (1971). A phase and electron microscopic study of vasculogenesis and erythropoiesis in the yolk sac of the mouse. The Anatomical Record 170, 199-223. crossref(new window)

32.
Han, J.K., Chang, S.H., Cho, H.J., Choi, S.B., Ahn, H.S., Lee, J., Jeong, H., Youn, S.W., Lee, H.J., Kwon, Y.W., et al. (2014). Direct conversion of adult skin fibroblasts to endothelial cells by defined factors. Circulation 130, 1168-1178. crossref(new window)

33.
Hart, A., Melet, F., Grossfeld, P., Chien, K., Jones, C., Tunnacliffe, A., Favier, R., and Bernstein, A. (2000). Fli-1 is required for murine vascular and megakaryocytic development and is hemizygously deleted in patients with thrombocytopenia. Immunity 13, 167-177. crossref(new window)

34.
Hatakeyama, J., and Kageyama, R. (2004). Retinal cell fate determination and bHLH factors. Semin. Cell Devel. Biol. 15, 83-89. crossref(new window)

35.
Hayashi, M., Pluchinotta, M., Momiyama, A., Tanaka, Y., Nishikawa, S., and Kataoka, H. (2012). Endothelialization and altered hematopoiesis by persistent Etv2 expression in mice. Exp. Hematol. 40, 738-750 e711. crossref(new window)

36.
Hirata, H., Kawamata, S., Murakami, Y., Inoue, K., Nagahashi, A., Tosaka, M., Yoshimura, N., Miyamoto, Y., Iwasaki, H., Asahara, T., et al. (2007). Coexpression of platelet-derived growth factor receptor alpha and fetal liver kinase 1 enhances cardiogenic potential in embryonic stem cell differentiation in vitro. J. Biosci. Bioeng. 103, 412-419. crossref(new window)

37.
Hollenhorst, P.C., Jones, D.A., and Graves, B.J. (2004). Expression profiles frame the promoter specificity dilemma of the ETS family of transcription factors. Nucleic Acids Res. 32, 5693-5702. crossref(new window)

38.
Hollenhorst, P.C., McIntosh, L.P., and Graves, B.J. (2011). Genomic and biochemical insights into the specificity of ETS transcription factors. Ann. Rev. Biochem. 80, 437-471. crossref(new window)

39.
Huang, P., He, Z., Ji, S., Sun, H., Xiang, D., Liu, C., Hu, Y., Wang, X., and Hui, L. (2011). Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors. Nature 475, 386-389. crossref(new window)

40.
Ieda, M., Fu, J.D., Delgado-Olguin, P., Vedantham, V., Hayashi, Y., Bruneau, B.G., and Srivastava, D. (2010). Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell 142, 375-386. crossref(new window)

41.
Iwafuchi-Doi, M., and Zaret, K.S. (2014). Pioneer transcription factors in cell reprogramming. Genes Dev. 28, 2679-2692. crossref(new window)

42.
Jain, R.K. (2003). Molecular regulation of vessel maturation. Nat. Med. 9, 685-693. crossref(new window)

43.
Johnson, N.C., Dillard, M.E., Baluk, P., McDonald, D.M., Harvey, N.L., Frase, S.L., and Oliver, G. (2008). Lymphatic endothelial cell identity is reversible and its maintenance requires Prox1 activity. Genes Dev. 22, 3282-3291. crossref(new window)

44.
Kataoka, H., Hayashi, M., Nakagawa, R., Tanaka, Y., Izumi, N., Nishikawa, S., Jakt, M.L., Tarui, H., and Nishikawa, S. (2011). Etv2/ER71 induces vascular mesoderm from Flk1+PDGFRalpha+ primitive mesoderm. Blood 118, 6975-6986. crossref(new window)

45.
Kim, H., Nguyen, V.P., Petrova, T.V., Cruz, M., Alitalo, K., and Dumont, D.J. (2010). Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature. BMC Dev. Biol. 10, 72. crossref(new window)

46.
Kim, J.Y., Lee, R.H., Kim, T.M., Kim, D.W., Jeon, Y.J., Huh, S.H., Oh, S.Y., Kyba, M., Kataoka, H., Choi, K., et al. (2014). OVOL2 is a critical regulator of ER71/ETV2 in generating FLK1+, hematopoietic, and endothelial cells from embryonic stem cells. Blood 124, 2948-2952. crossref(new window)

47.
Kissa, K., and Herbomel, P. (2010). Blood stem cells emerge from aortic endothelium by a novel type of cell transition. Nature 464, 112-115. crossref(new window)

48.
Knoepfler, P.S. (2009). Deconstructing stem cell tumorigenicity: a roadmap to safe regenerative medicine. Stem Cells 27, 1050-1056. crossref(new window)

49.
Kodandapani, R., Pio, F., Ni, C.Z., Piccialli, G., Klemsz, M., McKercher, S., Maki, R.A., Ely, K.R. (1996). A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex. Nature 380, 456-460. crossref(new window)

50.
Koyano-Nakagawa, N., Kweon, J., Iacovino, M., Shi, X., Rasmussen, T.L., Borges, L., Zirbes, K.M., Li, T., Perlingeiro, R.C., Kyba, M., et al. (2012). Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression. Stem Cells 30, 1611-1623. crossref(new window)

51.
Kume, T., Jiang, H., Topczewska, J.M., and Hogan, B.L. (2001). The murine winged helix transcription factors, Foxc1 and Foxc2, are both required for cardiovascular development and somitogenesis. Genes Dev. 15, 2470-2482. crossref(new window)

52.
Lee, D., Park, C., Lee, H., Lugus, J.J., Kim, S.H., Arentson, E., Chung, Y.S., Gomez, G., Kyba, M., Lin, S., et al. (2008). ER71 acts downstream of BMP, Notch, and Wnt signaling in blood and vessel progenitor specification. Cell Stem Cell 2, 497-507. crossref(new window)

53.
Lee, D., Kim, T., and Lim, D.S. (2011). The Er71 is an important regulator of hematopoietic stem cells in adult mice. Stem Cells 29, 539-548. crossref(new window)

54.
Lee, S., Park, C., Han, J.W., Kim, J.Y., Cho, K., Kim, E.J., Kim, S., Lee, S.-J., An, H.J., Sin, M.Y., et al. (2014). Abstract 18205: Direct Reprogramming of Human Dermal Fibroblasts into Endothelial Cells Using a Single Transcription Factor. Circulation 130, A18205.

55.
Lindsley, R.C., Gill, J.G., Murphy, T.L., Langer, E.M., Cai, M., Mashayekhi, M., Wang, W., Niwa, N., Nerbonne, J.M., Kyba, M., et al. (2008). Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs. Cell Stem Cell 3, 55-68. crossref(new window)

56.
Liu, F., Kang, I., Park, C., Chang, L.W., Wang, W., Lee, D., Lim, D.S., Vittet, D., Nerbonne, J.M., and Choi, K. (2012). ER71 specifies Flk-1+ hemangiogenic mesoderm by inhibiting cardiac mesoderm and Wnt signaling. Blood 119, 3295-3305. crossref(new window)

57.
Liu, F., Bhang, S.H., Arentson, E., Sawada, A., Kim, C.K., Kang, I., Yu, J., Sakurai, N., Kim, S.H., Yoo, J.J., et al. (2013). Enhanced hemangioblast generation and improved vascular repair and regeneration from embryonic stem cells by defined transcription factors. Stem Cell Rep. 1, 166-182. crossref(new window)

58.
Liu, F., Li, D., Yu, Y.Y., Kang, I., Cha, M.J., Kim, J.Y., Park, C., Watson, D.K., Wang, T., and Choi, K. (2015). Induction of hematopoietic and endothelial cell program orchestrated by ETS transcription factor ER71/ETV2. EMBO Rep. 16, 654-669. crossref(new window)

59.
Lugus, J.J., Chung, Y.S., Mills, J.C., Kim, S.I., Grass, J., Kyba, M., Doherty, J.M., Bresnick, E.H., and Choi, K. (2007). GATA2 functions at multiple steps in hemangioblast development and differentiation. Development 134, 393-405. crossref(new window)

60.
Lyons, I., Parsons, L.M., Hartley, L., Li, R., Andrews, J.E., Robb, L., and Harvey, R.P. (1995). Myogenic and morphogenetic defects in the heart tubes of murine embryos lacking the homeo box gene Nkx2-5. Genes Dev. 9, 1654-1666. crossref(new window)

61.
Meadows, S.M., Myers, C.T., and Krieg, P.A. (2011). Regulation of endothelial cell development by ETS transcription factors. Semin. Cell Dev. Biol. 22, 976-984. crossref(new window)

62.
Moore, J.C., Sheppard-Tindell, S., Shestopalov, I.A., Yamazoe, S., Chen, J.K., and Lawson, N.D. (2013). Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Dev. Biol. 384, 128-140. crossref(new window)

63.
Morita, R., Suzuki, M., Kasahara, H., Shimizu, N., Shichita, T., Sekiya, T., Kimura, A., Sasaki, K., Yasukawa, H., and Yoshimura, A. (2015). ETS transcription factor ETV2 directly converts human fibroblasts into functional endothelial cells. Proc. Natl. Acad. Sci. USA 112, 160-165. crossref(new window)

64.
Motoike, T., Markham, D.W., Rossant, J., and Sato, T.N. (2003). Evidence for novel fate of Flk1+ progenitor: contribution to muscle lineage. Genesis 35, 153-159. crossref(new window)

65.
Mozaffarian, D., Benjamin, E.J., Go, A.S., Arnett, D.K., Blaha, M.J., Cushman, M., de Ferranti, S., Despres, J.P., Fullerton, H.J., Howard, V.J., et al. (2015). Heart disease and stroke statistics--2015 update: a report from the American Heart Association. Circulation 131, e29-322. crossref(new window)

66.
Neuhaus, H., Muller, F., and Hollemann, T. (2010). Xenopus er71 is involved in vascular development. Dev. Dyn. 239, 3436-3445. crossref(new window)

67.
Palencia-Desai, S., Kohli, V., Kang, J., Chi, N.C., Black, B.L., and Sumanas, S. (2011). Vascular endothelial and endocardial progenitors differentiate as cardiomyocytes in the absence of Etsrp/Etv2 function. Development 138, 4721-4732. crossref(new window)

68.
Palis, J., Robertson, S., Kennedy, M., Wall, C., and Keller, G. (1999). Development of erythroid and myeloid progenitors in the yolk sac and embryo proper of the mouse. Development 126, 5073-5084.

69.
Pang, Z.P., Yang, N., Vierbuchen, T., Ostermeier, A., Fuentes, D.R., Yang, T.Q., Citri, A., Sebastiano, V., Marro, S., Sudhof, T.C., et al. (2011). Induction of human neuronal cells by defined transcription factors. Nature 476, 220-223.

70.
Park, C., Kim, T.M., and Malik, A.B. (2013). Transcriptional regulation of endothelial cell and vascular development. Circ. Res. 112, 1380-1400. crossref(new window)

71.
Park, C., Lee, T.J., Bhang, S.H., Liu, F., Nakamura, R., Oladipupo, S.S., Pitha-Rowe, I., Capoccia, B., Choi, H.S., Kim, T.M., et al. (2015). Injury-Mediated Vascular Regeneration Requires Endothelial ER71/ETV2. Arteriosclerosis, thrombosis, and vascular biology. Nov 19. pii: ATVBAHA.115.306430. [Epub ahead of print]

72.
Patan, S. (2004). Vasculogenesis and angiogenesis. Cancer Treat. Res. 117, 3-32. crossref(new window)

73.
Pham, V.N., Lawson, N.D., Mugford, J.W., Dye, L., Castranova, D., Lo, B., and Weinstein, B.M. (2007). Combinatorial function of ETS transcription factors in the developing vasculature. Dev. Biol. 303, 772-783. crossref(new window)

74.
Randi, A.M., Sperone, A., Dryden, N.H., and Birdsey, G.M. (2009). Regulation of angiogenesis by ETS transcription factors. Biochem. Soc. Trans. 37, 1248-1253. crossref(new window)

75.
Rasmussen, T.L., Kweon, J., Diekmann, M.A., Belema-Bedada, F., Song, Q., Bowlin, K., Shi, X., Ferdous, A., Li, T., Kyba, M., et al. (2011). ER71 directs mesodermal fate decisions during embryogenesis. Development 138, 4801-4812. crossref(new window)

76.
Sakurai, H., Era, T., Jakt, L.M., Okada, M., Nakai, S., Nishikawa, S., and Nishikawa, S. (2006). In vitro modeling of paraxial and lateral mesoderm differentiation reveals early reversibility. Stem Cells 24, 575-586. crossref(new window)

77.
Schoenebeck, J.J., Keegan, B.R., and Yelon, D. (2007). Vessel and blood specification override cardiac potential in anterior mesoderm. Dev. Cell 13, 254-267. crossref(new window)

78.
Sekiya, S., and Suzuki, A. (2011). Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475, 390-393. crossref(new window)

79.
Seo, S., Fujita, H., Nakano, A., Kang, M., Duarte, A., and Kume, T. (2006). The forkhead transcription factors, Foxc1 and Foxc2, are required for arterial specification and lymphatic sprouting during vascular development. Dev. Biol. 294, 458-470. crossref(new window)

80.
Sharrocks, A.D. (2001). The ETS-domain transcription factor family. Nat. Rev. Mol. Cell. Biol. 2, 827-837. crossref(new window)

81.
Shi, X., Richard, J., Zirbes, K.M., Gong, W., Lin, G., Kyba, M., Thomson, J.A., Koyano-Nakagawa, N., and Garry, D.J. (2014). Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages. Dev. Biol. 389, 208-218. crossref(new window)

82.
Shi, X., Zirbes, K.M., Rasmussen, T.L., Ferdous, A., Garry, M.G., Koyano-Nakagawa, N., and Garry, D.J. (2015). The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression. J. Biol. Chem. 290, 9614-9625. crossref(new window)

83.
Simoes, F.C., Peterkin, T., and Patient, R. (2011). Fgf differentially controls cross-antagonism between cardiac and haemangioblast regulators. Development 138, 3235-3245. crossref(new window)

84.
Song, K., Nam, Y.J., Luo, X., Qi, X., Tan, W., Huang, G.N., Acharya, A., Smith, C.L., Tallquist, M.D., Neilson, E.G., et al. (2012). Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature 485, 599-604. crossref(new window)

85.
Spyropoulos, D.D., Pharr, P.N., Lavenburg, K.R., Jackers, P., Papas, T.S., Ogawa, M., and Watson, D.K. (2000). Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. Mol. Cell. Biol. 20, 5643-5652. crossref(new window)

86.
Stainier, D.Y., Weinstein, B.M., Detrich, H.W., 3rd, Zon, L.I., and Fishman, M.C. (1995). Cloche, an early acting zebrafish gene, is required by both the endothelial and hematopoietic lineages. Development 121, 3141-3150.

87.
Stenman, J.M., Rajagopal, J., Carroll, T.J., Ishibashi, M., McMahon, J., and McMahon, A.P. (2008). Canonical Wnt signaling regulates organ-specific assembly and differentiation of CNS vasculature. Science 322, 1247-1250. crossref(new window)

88.
Sumanas, S., and Lin, S. (2006). Ets1-related protein is a key regulator of vasculogenesis in zebrafish. PLoS Biol. 4, e10.

89.
Sumanas, S., Jorniak, T., and Lin, S. (2005). Identification of novel vascular endothelial-specific genes by the microarray analysis of the zebrafish cloche mutants. Blood 106, 534-541. crossref(new window)

90.
Takahashi, K., and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676. crossref(new window)

91.
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872. crossref(new window)

92.
Takeuchi, M., Fuse, Y., Watanabe, M., Andrea, C.S., Takeuchi, M., Nakajima, H., Ohashi, K., Kaneko, H., Kobayashi-Osaki, M., Yamamoto, M., et al. (2015). LSD1/KDM1A promotes hematopoietic commitment of hemangioblasts through downregulation of Etv2. Proc. Natl. Acad. Sci. USA 112, 13922-13927. crossref(new window)

93.
Tanaka, M., Chen, Z., Bartunkova, S., Yamasaki, N., and Izumo, S. (1999). The cardiac homeobox gene Csx/Nkx2.5 lies genetically upstream of multiple genes essential for heart development. Development 126, 1269-1280.

94.
Tsai, F.Y., and Orkin, S.H. (1997). Transcription factor GATA-2 is required for proliferation/survival of early hematopoietic cells and mast cell formation, but not for erythroid and myeloid terminal differentiation. Blood 89, 3636-3643.

95.
Tsai, F.Y., Keller, G., Kuo, F.C., Weiss, M., Chen, J., Rosenblatt, M., Alt, F.W., and Orkin, S.H. (1994). An early haematopoietic defect in mice lacking the transcription factor GATA-2. Nature 371, 221-226. crossref(new window)

96.
Unezaki, S., Horai, R., Sudo, K., Iwakura, Y., and Ito, S. (2007). Ovol2/Movo, a homologue of Drosophila ovo, is required for angiogenesis, heart formation and placental development in mice. Genes Cells 12, 773-785.

97.
Veldman, M.B., and Lin, S. (2012). Etsrp/Etv2 is directly regulated by Foxc1a/b in the zebrafish angioblast. Circ. Res. 110, 220-229. crossref(new window)

98.
Veldman, M.B., Zhao, C., Gomez, G.A., Lindgren, A.G., Huang, H., Yang, H., Yao, S., Martin, B.L., Kimelman, D., and Lin, S. (2013). Transdifferentiation of fast skeletal muscle into functional endothelium in vivo by transcription factor Etv2. PLoS Biol. 11, e1001590. crossref(new window)

99.
Verger, A., and Duterque-Coquillaud, M. (2002). When Ets transcription factors meet their partners. Bioessays 24, 362-370. crossref(new window)