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Mitochondrial Efficiency-Dependent Viability of Saccharomyces cerevisiae Mutants Carrying Individual Electron Transport Chain Component Deletions
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  • Journal title : Molecules and Cells
  • Volume 38, Issue 12,  2015, pp.1054-1063
  • Publisher : Korea Society for Molecular and Cellular Biology
  • DOI : 10.14348/molcells.2015.0153
 Title & Authors
Mitochondrial Efficiency-Dependent Viability of Saccharomyces cerevisiae Mutants Carrying Individual Electron Transport Chain Component Deletions
Kwon, Young-Yon; Choi, Kyung-Mi; Cho, ChangYeon; Lee, Cheol-Koo;
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 Abstract
Mitochondria play a crucial role in eukaryotic cells; the mitochondrial electron transport chain (ETC) generates adenosine triphosphate (ATP), which serves as an energy source for numerous critical cellular activities. However, the ETC also generates deleterious reactive oxygen species (ROS) as a natural byproduct of oxidative phosphorylation. ROS are considered the major cause of aging because they damage proteins, lipids, and DNA by oxidation. We analyzed the chronological life span, growth phenotype, mitochondrial membrane potential (MMP), and intracellular ATP and mitochondrial superoxide levels of 33 single ETC component-deleted strains during the chronological aging process. Among the ETC mutant strains, 14 (, , , , , , , , , , , , , and ) showed a significantly shorter life span. The deleted genes encode important elements of the ETC components succinate dehydrogenase (complex II) and cytochrome c oxidase (complex IV), and some of the deletions lead to structural instability of the membrane--ATP synthase due to mutations in the stator stalk (complex V). These short-lived strains generated higher superoxide levels and produced lower ATP levels without alteration of MMP. In summary, ETC mutations decreased the life span of yeast due to impaired mitochondrial efficiency.
 Keywords
cellular ATP;electron transport chain;ETC-component single gene deletion;mitochondria;mitochondrial ROS;
 Language
English
 Cited by
1.
Caloric Restriction and Rapamycin Differentially Alter Energy Metabolism in Yeast, The Journals of Gerontology: Series A, 2017  crossref(new windwow)
2.
Caloric Restriction-Induced Extension of Chronological Lifespan Requires Intact Respiration in Budding Yeast, Molecules and Cells, 2017, 40, 4, 307  crossref(new windwow)
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The role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions, Scientific Reports, 2017, 7, 1  crossref(new windwow)
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