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Kinetic Approaches to Measuring Peroxiredoxin Reactivity
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  • Journal title : Molecules and Cells
  • Volume 39, Issue 1,  2016, pp.26-30
  • Publisher : Korea Society for Molecular and Cellular Biology
  • DOI : 10.14348/molcells.2016.2325
 Title & Authors
Kinetic Approaches to Measuring Peroxiredoxin Reactivity
Winterbourn, Christine C.; Peskin, Alexander V.;
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Peroxiredoxins are ubiquitous thiol proteins that catalyse the breakdown of peroxides and regulate redox activity in the cell. Kinetic analysis of their reactions is required in order to identify substrate preferences, to understand how molecular structure affects activity and to establish their physiological functions. Various approaches can be taken, including the measurement of rates of individual steps in the reaction pathway by stopped flow or competitive kinetics, classical enzymatic analysis and measurement of peroxidase activity. Each methodology has its strengths and they can often give complementary information. However, it is important to understand the experimental conditions of the assay so as to interpret correctly what parameter is being measured. This brief review discusses different kinetic approaches and the information that can be obtained from them.
hyperoxidation;kinetics;peroxide;peroxiredoxin;thiol oxidation;
 Cited by
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