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Structural Analysis of the Streptomyces avermitilis CYP107W1-Oligomycin A Complex and Role of the Tryptophan 178 Residue
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  • Journal title : Molecules and Cells
  • Volume 39, Issue 3,  2016, pp.211-216
  • Publisher : Korea Society for Molecular and Cellular Biology
  • DOI : 10.14348/molcells.2016.2226
 Title & Authors
Structural Analysis of the Streptomyces avermitilis CYP107W1-Oligomycin A Complex and Role of the Tryptophan 178 Residue
Han, Songhee; Pham, Tan-Viet; Kim, Joo-Hwan; Lim, Young-Ran; Park, Hyoung-Goo; Cha, Gun-Su; Yun, Chul-Ho; Chun, Young-Jin; Kang, Lin-Woo; Kim, Donghak;
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 Abstract
CYP107W1 from Streptomyces avermitilis is a cytochrome P450 enzyme involved in the biosynthesis of macrolide oligomycin A. A previous study reported that CYP107W1 regioselectively hydroxylated C12 of oligomycin C to produce oligomycin A, and the crystal structure of ligand free CYP107W1 was determined. Here, we analyzed the structural properties of the CYP107W1-oligomycin A complex and characterized the functional role of the Trp178 residue in CYP107W1. The crystal structure of the CYP107W1 complex with oligomycin A was determined at a resolution of . Oligomycin A is bound in the substrate access channel on the upper side of the prosthetic heme mainly by hydrophobic interactions. In particular, the Trp178 residue in the active site intercalates into the large macrolide ring, thereby guiding the substrate into the correct binding orientation for a productive P450 reaction. A Trp178 to Gly mutation resulted in the distortion of binding titration spectra with oligomycin A, whereas binding spectra with azoles were not affected. The Gly178 mutant`s catalytic turnover number for the 12-hydroxylation reaction of oligomycin C was highly reduced. These results indicate that Trp178, located in the open pocket of the active site, may be a critical residue for the productive binding conformation of large macrolide substrates.
 Keywords
CYP;CYP107W1;oligomycin;P450;Streptomyces avermitilis;X-ray crystal structure;
 Language
English
 Cited by
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Structural insights into the binding of lauric acid to CYP107L2 from Streptomyces avermitilis, Biochemical and Biophysical Research Communications, 2017, 482, 4, 902  crossref(new windwow)
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Characterization of a Biflaviolin Synthase CYP158A3 fromStreptomyces avermitilisand Its Role in the Biosynthesis of Secondary Metabolites, Biomolecules & Therapeutics, 2017, 25, 2, 171  crossref(new windwow)
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Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function, Nat. Prod. Rep., 2017, 34, 9, 1141  crossref(new windwow)
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Recent Structural Insights into Cytochrome P450 Function, Trends in Pharmacological Sciences, 2016, 37, 8, 625  crossref(new windwow)
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