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miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs
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  • Journal title : Molecules and Cells
  • Volume 39, Issue 4,  2016, pp.299-309
  • Publisher : Korea Society for Molecular and Cellular Biology
  • DOI : 10.14348/molcells.2016.2242
 Title & Authors
miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs
Park, Deokbum; Kim, Hyuna; Kim, Youngmi; Jeoung, Dooil;
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We have previously reported the role of miR-217 in anti-cancer drug-resistance. miRNA array and miRNA hybridization analysis predicted miR-30a-3p as a target of miR-217. miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217. miR-30a-3p conferred resistance to anti-cancer drugs and enhanced the invasion, migration, angiogenic, tumorigenic, and metastatic potential of cancer cells in CAGE-dependent manner. CAGE increased the expression of miR-30a-3p by binding to the promoter sequences of miR-30a-3p, suggesting a positive feedback loop between CAGE and miR-30a-3p. miR-30a-3p decreased the expression of p53, which showed the binding to the promoter sequences of miR-30a-3p and CAGE in anti-cancer drug-sensitive cancer cells. Luciferase activity assays showed that p53 serves as a target of miR-30a. Thus, the miR-30a-3p-CAGE-p53 feedback loop serves as a target for overcoming resistance to anti-cancer drugs.
anti-cancer drug-resistance;CAGE;feedback loop;miR-30a;p53;
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