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Dual Regulation of R-Type CaV2.3 Channels by M1 Muscarinic Receptors
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  • Journal title : Molecules and Cells
  • Volume 39, Issue 4,  2016, pp.322-329
  • Publisher : Korea Society for Molecular and Cellular Biology
  • DOI : 10.14348/molcells.2016.2292
 Title & Authors
Dual Regulation of R-Type CaV2.3 Channels by M1 Muscarinic Receptors
Jeong, Jin-Young; Kweon, Hae-Jin; Suh, Byung-Chang;
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 Abstract
Voltage-gated () channels are dynamically modulated by Gprotein-coupled receptors (GPCR). The muscarinic receptor stimulation is known to enhance channel gating through the activation of protein kinase C (PKC). Here, we found that receptors also inhibit currents when the channels are fully activated by PKC. In whole-cell configuration, the application of phorbol 12-myristate 13-acetate (PMA), a PKC activator, potentiated currents by ~two-fold. After the PMA-induced potentiation, stimulation of receptors decreased the currents by . We examined whether the depletion of phosphatidylinositol 4,5-bisphosphate () is responsible for the muscarinic suppression of currents by using two methods: the Danio rerio voltage-sensing phosphatase (Dr-VSP) system and the rapamycin-induced translocatable pseudojanin (PJ) system. First, dephosphorylation of to phosphatidylinositol 4-phosphate (PI(4)P) by Dr-VSP significantly suppressed currents, by . Next, dephosphorylation of both PI(4)P and to PI by PJ translocation further decreased the current by up to . The results suggest that currents are modulated by the receptor in a dual mode-that is, potentiation through the activation of PKC and suppression by the depletion of membrane . Our results also suggest that there is rapid turnover between PI(4)P and in the plasma membrane.
 Keywords
channel;Danio rerio voltage-sensitive phosphatase(Dr-VSP); muscarinic receptor;;Pseudojanin;
 Language
English
 Cited by
1.
Stimulatory and inhibitory effects of PKC isozymes are mediated by serine/threonine PKC sites of the Ca v 2.3α 1 subunits, Archives of Biochemistry and Biophysics, 2017, 621, 24  crossref(new windwow)
2.
The HOOK region of voltage-gated Ca2+channel β subunits senses and transmits PIP2signals to the gate, The Journal of General Physiology, 2017, 149, 2, 261  crossref(new windwow)
3.
PI(4,5)P 2 and L-type Ca 2+ Channels Partner Up to Fine-Tune Ca 2+ Dynamics in β Cells, Cell Chemical Biology, 2016, 23, 7, 753  crossref(new windwow)
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