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MicroRNA Target Recognition: Insights from Transcriptome-Wide Non-Canonical Interactions
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  • Journal title : Molecules and Cells
  • Volume 39, Issue 5,  2016, pp.375-381
  • Publisher : Korea Society for Molecular and Cellular Biology
  • DOI : 10.14348/molcells.2016.0013
 Title & Authors
MicroRNA Target Recognition: Insights from Transcriptome-Wide Non-Canonical Interactions
Seok, Heeyoung; Ham, Juyoung; Jang, Eun-Sook; Chi, Sung Wook;
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 Abstract
MicroRNAs (miRNAs) are small non-coding RNAs (~22 nucleotides) regulating gene expression at the post-transcriptional level. By directing the RNA-induced silencing complex (RISC) to bind specific target mRNAs, miRNA can repress target genes and affect various biological phenotypes. Functional miRNA target recognition is known to majorly attribute specificity to consecutive pairing with seed region (position 2-8) of miRNA. Recent advances in a transcriptome-wide method of mapping miRNA binding sites (Ago HITS-CLIP) elucidated that a large portion of miRNA-target interactions in vivo are mediated not only through the canonical "seed sites" but also via non-canonical sites (~15-80%), setting the stage to expand and determine their properties. Here we focus on recent findings from transcriptome-wide non-canonical miRNA-target interactions, specifically regarding "nucleation bulges" and "seed-like motifs". We also discuss insights from Ago HITS-CLIP data alongside structural and biochemical studies, which highlight putative mechanisms of miRNA target recognition, and the biological significance of these non-canonical sites mediating marginal repression.
 Keywords
argonaute;CLIP;microRNA;non-canonical targets;
 Language
English
 Cited by
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