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Arginine Supplementation Recovered the IFN-γ-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2α Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells
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  • Journal title : Molecules and Cells
  • Volume 39, Issue 5,  2016, pp.410-417
  • Publisher : Korea Society for Molecular and Cellular Biology
  • DOI : 10.14348/molcells.2016.2358
 Title & Authors
Arginine Supplementation Recovered the IFN-γ-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2α Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells
Xia, Xiaojing; Che, Yanyi; Gao, Yuanyuan; Zhao, Shuang; Ao, Changjin; Yang, Hongjian; Liu, Juxiong; Liu, Guowen; Han, Wenyu; Wang, Yuping; Lei, Liancheng;
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During the lactation cycle of the bovine mammary gland, autophagy is induced in bovine mammary epithelial cells (BMECs) as a cellular homeostasis and survival mechanism. Interferon gamma () is an important antiproliferative and apoptogenic factor that has been shown to induce autophagy in multiple cell lines in vitro. However, it remains unclear whether can induce autophagy and whether autophagy affects milk synthesis in BMECs. To understand whether affects milk synthesis, we isolated and purified primary BMECs and investigated the effect of on milk synthesis in primary BMECs in vitro. The results showed that significantly inhibits milk synthesis and that autophagy was clearly induced in primary BMECs in vitro within 24 h. Interestingly, autophagy was observed following treatment, and the inhibition of autophagy can improve milk protein and milk fat synthesis. Conversely, upregulation of autophagy decreased milk synthesis. Furthermore, mechanistic analysis confirmed that mediated autophagy by depleting arginine and inhibiting the general control nonderepressible-2 kinase (GCN2)/eukaryotic initiation factor () signaling pathway in BMECs. Then, it was found that arginine supplementation could attenuate -induced autophagy and recover milk synthesis to some extent. These findings may not only provide a novel measure for preventing the -induced decrease in milk quality but also a useful therapeutic approach for -associated breast diseases in other animals and humans.
autophagy;arginine;BMECs;;milk synthesis;
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