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PLZF+ Innate T Cells Support the TGF-β-Dependent Generation of Activated/Memory-Like Regulatory T Cells
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  • Journal title : Molecules and Cells
  • Volume 39, Issue 6,  2016, pp.468-476
  • Publisher : Korea Society for Molecular and Cellular Biology
  • DOI : 10.14348/molcells.2016.0004
 Title & Authors
PLZF+ Innate T Cells Support the TGF-β-Dependent Generation of Activated/Memory-Like Regulatory T Cells
Kang, Byung Hyun; Park, Hyo Jin; Park, Hi Jung; Lee, Jae-Il; Park, Seong Hoe; Jung, Kyeong Cheon;
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PLZF-expressing invariant natural killer T cells and CD4 T cells are unique subsets of innate T cells. Both are selected via thymocyte-thymocyte interaction, and they contribute to the generation of activated/memory-like CD4 and CD8 T cells in the thymus via the production of IL-4. Here, we investigated whether innate T cells also affect the development and function of regulatory CD4 T cells. Flow cytometry analysis of the thymus and spleen from both CIITA transgenic C57BL/6 and wild-type BALB/c mice, which have abundant CD4 T cells and invariant natural killer T cells, respectively, revealed that T cells in these mice exhibited a activated/memorylike phenotype. The frequency of regulatory T cells was considerably decreased in cell-deficient and mice as well as in an IL-4-deficient background, such as in and mice, indicating that the acquisition of an activated/ memory-like phenotype was dependent on innate T cells and IL-4. Using fetal thymic organ culture, we further demonstrated that IL-4 in concert with TGF- enhanced the acquisition of the activated/memory-like phenotype of regulatory T cells. In functional aspects, the activated/ memory-like phenotype of Treg cells was directly related to their suppressive function; regulatory T cells of mice more efficiently suppressed ovalbumin-induced allergic airway inflammation compared with their counterparts from wild-type mice. All of these findings suggest that innate T cells also augmented the generation of activated/memory-like regulation via IL-4 production.
IL-4;PLZF;regulatory T lymphocyte;
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