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Rapid Sex Identification of Chicken by Fluorescence In Situ Hybridization Using a W Chromosome-specific DNA Probe
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 Title & Authors
Rapid Sex Identification of Chicken by Fluorescence In Situ Hybridization Using a W Chromosome-specific DNA Probe
Sohn, S.H.; Lee, C.Y.; Ryu, E.K.; Han, J.Y.; Multani, A.S.; Pathak, S.;
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It has been known that the sex of chicken cells can be most accurately identified by fluorescence in situ hybridization (FISH). However, the presently available FISH has not been widely used for sex identification, because the procedures for cell preparation and FISH itself are complicated and time-consuming. The present study was undertaken to test a rapid FISH procedure for sexing chicken. A FISH probe was simultaneously synthesized and labeled with digoxigenin by polymerase chain reaction (PCR) targeting a 416 bp segment of the 717 bp XhoI family fragment which is repeated over 10 thousand times exclusively in the W chromosome. Sexing by FISH was performed on cytological preparations of early embryos, adult lymphocytes and feather pulps of newly hatched chicks. The DNA probe hybridized to all types of uncultured interphase as well as metaphase female but not male cells that had been examined. Moreover, consistent with the known site of the XhoI family, the hybridization signal was localized to the pericentromeric region of the W chromosome. We, therefore, conclude that the present PCR-based FISH can be used as a rapid and reliable sex identification procedure for chicken.
Chicken;W Chromosome;XhoI family;PCR;FISH;Sexing;
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Clinton, M. 1994. A rapid protocol for sexing chick embryos (Gallus g. domesticus). Anim. Genet. 25:361-362. crossref(new window)

Griffiths, R., M. C. Double, K. Orr and R. J. G. Dawson. 1998. A DNA test to sex most birds. Mol. Ecol. 7:1071-1075. crossref(new window)

Itoh, Y., M. Suzuki, A. Ogawa, I. Munechika, K. Murata and S. Mizuno. 2001. Identification the sex of a wide range of Carinatae birds by PCR using primer sets selected from chicken EE0.6 and its related sequences. J. Hered. 92:315-321. crossref(new window)

Klein, S. and F. Ellendorff. 2000. Localization of XhoI repetitive sequences on autosomes in addition to the W chromosome in chickens and its relevance for sex diagnosis. Anim. Genet. 31:104-109. crossref(new window)

Kobayashi, J., S. Akiyo, U. Hiroshi, W. Tukasa, S. Kohsuke, S. Hiroshi, U. Motoaki and S. Eimei. 1998. Rapid detection of male-specific DNA sequence in bovine embryos using fluorescence in situ hybridization. Mol. Reprod. and Develop. 51:390-394. crossref(new window)

Kodama, H., H. Saitoh, M. Tone, S. Kuhara, Y. Sakaki and S. Mizuno. 1987. Nucleotide sequences and unusual electrophoretic behaviour of the W chromosome-specific repeating DNA units of the domestic fowl, Gallus gallus domesticus. Chromosoma 96:18-25. crossref(new window)

Mizuno, S. and H. Macgregor. 1998. The lampbrush chromosomes of birds: a unique opportunity to look at the molecular cytogenetics of sex chromosomes. Cytogenet. Cell Genet. 80:149-157. crossref(new window)

Ogawa, A., I. Solovei, N. Hutchison, Y. Saitoh, J. Ikeda, H. Macgregor and S. Mizuno. 1997. Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W chromosome of chicken and its use as a universal probe for sexing Carinatae birds. Chromosome Res. 5:93-101. crossref(new window)

Petitte, J. N. and A. E. Kegelmeyer. 1995. Rapid sex determination of chick embryos using the polymerase chain reaction. Anim. Biotech. 6:119-130. crossref(new window)

Saitoh, Y. and S. Mizuno. 1992. Distribution of XhoI and EcoRI family repetitive DNA sequences into separate domains in the chicken W chromosome. Chromosoma 101:474-477. crossref(new window)

Saitoh, Y., H. Saitoh, K. Ohtomo and S. Mizuno. 1991. Occupancy of the majority of DNA in the chicken W chromosome by bent-repetitive sequences. Chromosoma 101:32-40. crossref(new window)

Sanger, F., S. Nicklen and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:5463-5467. crossref(new window)

Seabright, M. 1971. A rapid banding technique for human chromosomes. Lancet 30:971-972. crossref(new window)

Sohn, S. H. and E. K. Ryu. 1999. Chromosome preparation from chick leucocyte culture using ficoll treatment. J. Agric. Technol. Res. (Jinju National University) 12:131-140.

Sohn, S. H., E. K. Ryu and K. H. Baik. 2000. A methodological study on the chromosome preparation for chick embryo; Induction of high-resolution chromosomes by using ethidium bromide. Korean J. Anim. Sci. Technol. 42:157-164.

Sohn, S. H., N. S. Fechheimer and K. E. Nestor. 1995. Transmission of C-band variants in Japanese quail. Asian-Aus. J. Anim. Sci. 8:171-174.

Suka, N., Y. Shinohara, Y. Saitoh, H. Saitoh, K. Ohtomo, M. Harata, E. Shpigelman and S. Mizuno. 1993. Wheterochromatin of chicken; its unusual DNA components, late replication, and chromatin structure. Genetica 88:93-105. crossref(new window)