JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Diluent Component, Freezing Rate, Thawing Time and Thawing Temperature on Acrosome Morphology and Motility of Frozen-thawed Boar Sperm
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Diluent Component, Freezing Rate, Thawing Time and Thawing Temperature on Acrosome Morphology and Motility of Frozen-thawed Boar Sperm
Yi, Y.J.; Kwon, Y.A.; Ko, H.J.; Park, C.S.;
  PDF(new window)
 Abstract
This study was carried out to obtain informations regarding the effect of N-acetyl-D-glucosamine in the LEY (lactoseegg yolk) diluent according to incubation time in 5 ml maxi-straw and the effects of freezing rate, thawing temperature and thawing time in the LEN (lactose-egg yolk and N-acetyl-D-glucosamine) diluent on acrosome morphology and motility of frozen-thawed boar sperm. The study showed that the LEN diluent was higher post-thaw NAR (normal apical ridge) acrosome than the LEY diluent for 0.5 h incubation at 37. However, there were no differences between the LEN and LEY diluents on post-thaw sperm motility according to incubation time. The straws frozen from 5.0 cm (20/min) to 17.0 cm (1/min) above the liquid nitrogen surface did not show any significant differences on post-thaw sperm motility. However, the straws frozen above 5.0 cm from the liquid nitrogen surface were higher NAR acrosome than those frozen above 17.0 cm. The post-thaw percentages of motile sperm and NAR acrosome were significantly higher (p<0.05) for the maxi-straws submerged for 40 or 45 sec in a 52 water bath than for 30, 35, 50 or 55 sec. The mean sample temperatures of maxi-straws after 40 or 45 sec submersion were 20.7 or 26.4. In conclusion, the sample temperature of the thawed semen was very important for post-thaw sperm survival in the LEN diluent of 5 ml maxi-straw. When the temperature of the thawed semen was 20.7, the percentages of motile sperm and NAR acrosome were highest.
 Keywords
Diluent;Freezing Rate;Thawing Temperature;Boar Sperm;
 Language
English
 Cited by
1.
Analysis of Membrane Integrity and Mitochondrial Activity in Fresh and Cryopreserved Boar Sperm Using Flow Cytometry,;;;;;;;;

Reproductive and Developmental Biology, 2005. vol.29. 4, pp.253-257
2.
종모돈의 정액성상, 동결-융해 후 정자의 생존성, 혈청 중 FSH, LH, Estradiol-17$\beta$ 및 Testosterone 농도에 미치는 품종과 계절의 영향,박창식;성낙도;김철호;진동일;최양석;이영주;

Reproductive and Developmental Biology, 2005. vol.29. 1, pp.25-30
3.
Evaluation of Boar Sperm Viability by MTT Reduction Assay in Beltsville Thawing Solution Extender,;;;;;;

아세아태평양축산학회지, 2008. vol.21. 4, pp.494-498 crossref(new window)
4.
Comparison of Viability, ATP and In vitro Fertilization of Boar Sperm Stored at 4℃ in the Three Different Diluents,;;;;;;;;;

아세아태평양축산학회지, 2008. vol.21. 8, pp.1127-1133 crossref(new window)
5.
Comparison of Motility, Acrosome, Viability and ATP of Boar Sperm with or without Cold Shock Resistance in Liquid Semen at 17℃ and 4℃, and Frozen-thawed Semen,;;;;;;;;

아세아태평양축산학회지, 2008. vol.21. 2, pp.190-197 crossref(new window)
6.
Simplified Slow Freezing Program Established for Effective Banking of Embryonic Stem Cells,;;;;;

아세아태평양축산학회지, 2009. vol.22. 3, pp.343-349 crossref(new window)
 References
1.
Aumuller, R. 1982. Tiefgefrierung von Ebersamen in Kunststoffrohren. Uberprufung des Einflusses der Zentrifugationstechnik im Besamungsversuch unter Praxisbedingungen. Hannover. Tieraztl. Hochschule, Diss.

2.
Crabo, B. G. and S. Einarsson. 1971. Fertility of deep frozen boar spermatozoa. Acta Vet. Scand. 12:125-127.

3.
Crabo, B. G., E. F. Graham and E. V. Larsson. 1972. The influence of thawing methods on frozen boar spermatozoa. Cryobiology 9: 331(Abstr.). crossref(new window)

4.
Eriksson, B., N. Lundeheim and H. Roderiguez-Martinez. 1997a. Viability of cryopreserved boar spermatozoa frozen in flat plastic containers. In: Control of Pig Reproduction (Ed. G. R. Foxcraft, R. D. Geisert and C. Doberska). J. Reprod. Fertil. (Suppl.) 52, p. 127.

5.
Eriksson. B., H. Ekwall and H. Rodriguez-Martinez. 1997b. Effect of freezing rates on the architecture of the frozen state and the post thaw viability of boar semen. In Proceedings of 34th Annual Meeting of the Society for Cryobiology, p. 142.

6.
Fiser, P. S. and R. W. Fairfull. 1990. Combined effect of glycerol concentration and cooling velocity on motility and acrosomal integrity of boar spermatozoa frozen in 0.5 ml straws. Mol. Reprod. Dev. 25:123-129. crossref(new window)

7.
Froede-Garibay, D. 1979. Tiefgefrierkonservierung von Ebersamen in Kunststoffrohren. Einflub der Zentrifugationstechnik auf qualitative und quantitatives Tiefgefriersperma-Parameter. Hannover. Tieraztl. Hochschule, Diss.

8.
Graham, E. F., A. H. Rajamannan, Jr., M. K. L. Schmehl, M. Maki-Laurila and R. E. Bowere. 1971. Preliminary reports on produce and rational for freezing boar semen. A. I. Digest 19:12.

9.
Kim, H. K., M. S. Ko, I. C. Kim, H. K. Chung, K. W. Lee, D. S. Son, H. Kim, S. H. Chee and C. S. Park. 1989. Deep freezing of boar semen in liquid nitrogen container. Korean J. Anim. Reprod. 31(3):155-157.

10.
Larsson, K. and S. Einarsson. 1976. Fertility of deep frozen boar spermatozoa. Influence of thawing diluents and of boars. Acta Vet. Scand. 17:43-62.

11.
Lehninger, A. L. 1975. Biochemistry Lehninger. Worth Publishers Inc. p. 266.

12.
Mazur, P. 1985. Basic concepts in freezing cells. In: Deep Freezing of Boar Semen (Ed. L. A. Johnson and K. Larsson). Swedish University of Agricultural Sciences, Uppsala, pp. 91-111.

13.
Medrano, A., W. V. Holt and P. F. Watson. 1998. The effect of freezing rate on inter-boar sperm cryosurvival. J. Reprod. Fertil. Abstract series no. 21, 114(Abstr.).

14.
Nagase, H. and T. Niwa. 1963. Studies on the deep freezing technique for bull semen. III. Deep freezing of bull semen in pellet form. Jap. J. Anim. Reprod. 9:73-77.

15.
Obando, H., T. Tamayo and C. Alvaro. 1984. Evaluation of some factors affecting swine spermatozoa during freezing. Xth Intern. cong. Anim. Reprod. AI, Urbana, II. p. 193.

16.
Paquignon, M. and M. Courot. 1976. Fertilizing capacity of frozen boar spermatozoa. Ⅷth Intl. Congr. Anim Reprod. A. I., Cracow, Ⅳ. pp. 1041-1044.

17.
Park, C. S. and V. G. Pursel. 1985. Effect of freezing rate on boar sperm frozen in maxi-straws. J. Anim. Sci. 61(Suppl. 1):411 (Abstr.).

18.
Perezcanto-Fernandez, J. 1978. Tiefgefrierkonservierung von Ebersamen in Kunststoffrohren. Einfuhrung des Verfahrens an einer Schweinebesamungsstation, in vitro Untersuchungen zur Nachverdunnerzusammensetzung und zum Auftauverfahren sowie Befruchtungsergebnisse nach Einsatz zweier Nachverdunner und der hormonellen Brunstinduktion. Hannover. Tieraztl. Hochschule, Diss.

19.
Polge, C., S. Salamon and I. Wilmut. 1970. Fertilizing capacity of frozen boar semen following surgical insemination. Vet. Rec. 87:424-428.

20.
Pursel, V. G. and L. A. Johnson. 1971. Procedure for the preservation of boar spermatozoa by freezing. USDA ARS Bull. No. 44-227, pp. 1-5.

21.
Pursel, V. G., L. A. Johnson and G. B. Rampacek. 1972. Acrosome morphology of boar spermatozoa incubated before cold shock. J. Anim. Sci. 34:278-283.

22.
Pursel, V. G. and L. A. Johnson. 1975. Freezing of boar spermatozoa: Fertilizing capacity with concentrated semen and a new thawing procedure. J. Anim. Sci. 40:99-102.

23.
Pursel, V. G. and L. A. Johnson. 1976. Frozen boar spermatozoa: Methods of thawing pellets. J. Anim. Sci. 42:927-931.

24.
Pursel, V. G., L. L. Schulman and L. A. Johnson. 1978. Effect of orvus es paste on acrosome morphology, motility and fertilizing capacity of frozen-thawed boar semen. J. Anim. Sci. 47(1):198-202.

25.
Pursel, V. G. and C. S. Park. 1985. Freezing and thawing procedures for boar spermatozoa. In: Deep Freezing of Boar Semen (Ed. L. A. Johnson and K. Larsson). Swedish University of Agricultural Sciences, Uppsala, pp. 147-166.

26.
Pursel, V. G. and C. S. Park. 1987. Duration of thawing on post thaw acrosome morphology and motility of boar spermatozoa frozen in 5 ml maxi-straws. Theriogenology 28(5):683-690. crossref(new window)

27.
Robbins, R. K., R. G. Saacke and P. T. Chandler. 1976. Influence of freeze rate, thaw rate and glycerol level on acrosomal retention and survival of bovine spermatozoa frozen in french straws. J. Anim. Sci. 42:145-154.

28.
Rota, A., C. Linde-Forsberg, J. Vannozzi, S. Romagnoli and H. Rodriguez-Martinez. 1998. Cryosurvival of dog spermatozoa at different glycerol concentrations and freezing/thawing rates. Reprod. Dom. Anim. 33:355-361. crossref(new window)

29.
SAS Institute Inc. 1988. SAS User's Guide: Statistics (version 6.03), SAS Inst. Inc., Cary, NC., USA.

30.
Scheid, I. R., P. Westendorf and H. Treu. 1980. Deep freezing of boar semen in plastic tubes ('Hulsenberg-Pailletten'): Effect of different glycerol concentrations. 6th Int. Pig Vet. Cong. Copenhagen, Denmark, p. 38.

31.
Schorner, G. 1974. Tiefkuhlkonservierung von Ebersamen. Wein. Tierarztl. Mschr. 61: 141-144.

32.
Schuler, D., U. Kupfer and F. Zimmerman. 1979. Tiefgefrieren von ebersperma: Ein Vergleich zweier Methoden. Schweiz. Arch. Tierheilk. 121:173-178.

33.
Visser, D. and S. Salamon. 1974. Effect of composition of trisbased diluent on survival of boar spermatozoa following deepfreezing. Aust. J. Biol. Sci. 27:485-497.

34.
Waide, Y. 1975. Fertility and survival of frozen boar spermatozoa stored in aluminum packaging containers. Jap. Agric. Res. Quart. 9:115-119.

35.
Watson, P. F. 1979. The preservation of semen in mammals. In: Oxford Reviews of Reproductive Biology (C. A. Finn). Vol. 1, Oxford University Press, Oxford, pp. 283-350.

36.
Westendorf, P., L. Richter and H. Teru. 1975. Zur Tiefgefrierung von Ebersperma. Labor-und Besamungsergebnisse mit dem Hulsenberger Pailletten-Verfahren. Deutsch Tierartzl Wochenschr. 82:261-367.

37.
Woelders, H. and M. Den Besten. 1993. Cryopreservation of boar semen with small between-boar variation of post-thaw sperm survival. Cryobiology 30, 645.

38.
Yi, Y. J., Y. M. Cheon and C. S. Park. 2002. Effect of N-acetyl-Dglucosamine, and glycerol concentration and equilibration time on acrosome morphology and motility of frozen-thawed boar sperm. Anim. Reprod. Sci. 69:91-97. crossref(new window)