Advanced SearchSearch Tips
Dynamics of Early Fermentation of Italian Ryegrass (Lolium multiflorum Lam.)Silage
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Dynamics of Early Fermentation of Italian Ryegrass (Lolium multiflorum Lam.)Silage
Shao, Tao; Ohba, N.; Shimojo, M.; Masuda, Y.;
  PDF(new window)
The dynamics of fermentation were studied with Italian ryegrass ensiled in the laboratory silos. The silos were kept in the room set at 25, and then were opened on 0.5, 1, 2, 3, 5, 7 and 14 days after ensiling, respectively. The samples were taken from three silos at each sampling time for chemical analyses. Mono-and disaccharides composition was determined for glucose, fructose and sucrose by high performance liquid chromatography. The Italian ryegrass silage succeeded to achieve lactate type fermentation; high values of lactic acid (85.83 g/kg) and lactic acid/acetic acid at the end of ensiling (14 day), low values of pH (3.74), acetic acid (5.38 g/kg), ethanol (19.20 g/kg) and (75.84 g/kg), no or only small amounts of butyric acid, valeric acid and propionic acid. The fermentation dynamics showed a fast and large pH decrease caused by a fast and large production of lactic acid during the first 5 days. Mono-and disaccharides composition largely decreased within initial 0.5 day (12 h) of ensiling. Sucrose disappeared rapidly within initial 0.5 day of ensiling, and fructose and glucose contents showed an initial rise during ensiling, and then decreased gradually. These indicated that the enzymes of plant tissue were active within 2 days of ensiling, which caused the initial rise in fructose and glucose from the hydrolysis of sucrose and fructans. After 5 days of ensilage, glucose was consumed completely, suggesting that glucose was the first fermentation substrate. After 2 days of ensiling, sum amounts of lactic acid and remaining mono-and disaccharides proved to be larger than the quantity of mono-and disaccharides in the initial grass. From the facts mentioned above, it was suggested that considerable amounts of lactic acid were produced from some other substrate such as fructans than initial mono-and disaccharides.
Dynamics;Fermentation;Italian Ryegrass;
 Cited by
Fermentation Quality of Italian Ryegrass (Lolium multiflorum Lam.) Silages Treated with Encapsulated-glucose, Glucose, Sorbic Acid and Pre-fermented Juices,;;;;

아세아태평양축산학회지, 2007. vol.20. 11, pp.1699-1704 crossref(new window)
Effect of Different Rates of Ethanol Additive on Fermentation Quality of Napiergrass (Pennisetum purpureum),;;;;

아세아태평양축산학회지, 2011. vol.24. 5, pp.636-642 crossref(new window)
Effect of inclusion of grasses and wet hulless-barley distillers? grains on the fermentation and nutritive quality of oat straw- and straw-grass silages in Tibet, Animal Production Science, 2013, 53, 5, 419  crossref(new windwow)
Effects of adding acetic acid and molasses on fermentation quality and aerobic stability of total mixed ration silage prepared with hulless barley straw in Tibet, Grassland Science, 2014, 60, 4, 206  crossref(new windwow)
degradability in Tibet, Grassland Science, 2016, 62, 4, 248  crossref(new windwow)
AOAC. 1984. Official Methods of Analysis. 14th edn. Association of Official and Analytical Chemists Arlington, Virginia-2221.

Ashbell, G. and Y. Kashanchi. 1987. In-silo losses from wheat ensiled in bunker silos in subtropical climate. J. Sci. Food Agric. 40:95-103. crossref(new window)

Barker, S. B. and W. H. Summerson. 1941. The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 138:535-554.

Beck, T. 1972. Die quantitative und qualitative Zusammensetzung der Milchsauerebakterienpopulation im Garfutter. Landwirtschaftliche Forschung. 27:55-63.

Beck, T. 1978. The microbiology of silage fermentation. In: Fermentation of Silage-a Review Ed. McCullough and M. E. Iowa: National Feed Ingredients Association. pp. 61-115.

Bousset, J., N. Bousset-Fatianoff, Ph., Gouet and M. Contrepois. 1972. Annales de biologie Animale, Biochimie et Biophysique. 12:453-477. crossref(new window)

Breese, E. L. 1983. Exploitation of genetic resource through breeding: Lolium species. In: Genetic Resources of Forage Plants (Ed. G. Mclover and R. A. Bray). CSIRO 1983, Australia. 275-288.

Carpintero, M. C., A. J. Holding and P. McDonald. 1969. Fermentation studies of Lucerne. J. Sci. Food Agric. 29:497-505. crossref(new window)

Catchpoole, V. R. and E. F. Henzell. 1971. Silage and silage making from tropical herbage species. Herb. Abstr. 41:213-219.

Clark, B. J. 1974. Ph. D. Thesis, University of Edinburgh.

Driehuis, F., P. G. Van. Wilkselaar, A. M. Van. Vuuren and S. F. Spoelstra. 1997. Effect of a bacteria inoculant on rate of fermentation and chemical composition of high dry matter grass silages. J Agric. Sci. Cambridge. 128:323-329. crossref(new window)

Gibson, T., A. C. Stirling, R. M. Keddie and R. F. Rosenberger. 1961. Bacteriological changes in silage as affected by laceration of the fresh grass. J. Appl. Bacteriol. 24:60-70.

Gouet, Ph., N. Fatianoff and J. Bousset. 1970. Compte Rendu del Academie de Sciences, Paris. 270:1024-1027.

Greenhill, W. L. 1964 a. Plant juices in relation to silage fermentation. I. The role of the juice. J. Br. Grassl. Soc. 19:30-37. crossref(new window)

Greenhill, W. L. 1964b. Plant juices in relation to silage fermentation. II. Factors affecting the release of juices. J. Br. Grassl. Soc. 19:231-236. crossref(new window)

Greenhill, W. L. 1964 c. Plant juices in relation to silage fermentation. III. Effect of water activity of juice. J. Br. Grassl. Soc. 19:231-236. crossref(new window)

Hattori, I., S. Kumai and R. Fukumi. 1993. The effect of saccharide additives on the fermentation quality of silage. J. Japan. Grassl. Sci. 39:326-333.

Henderson, A. R. and P. McDonald. 1971. Effect of formic acid on the fermentation of grass of low dry matter content. J. Sci. Food Agric. 22:157-163. crossref(new window)

Henderson, N. 1993. Silage additives. Anim. Feed Sci. Technol. 45:35-36. crossref(new window)

Masaki, S. and Y. Ohyama. 1979. Changes in sugars during ensilage-production of lactic acid and volatile fatty acids. Jap. J. Zootech. Sci. 50(5):280-287.

McDonald, P., A. R. Henderson and S. J. E. Heron. 1991. The Biochemistry of Silage. 2nd ed. Cambrian Printers Ltd. Aberystwyth. pp. 184-236.

Rooke, J. A. and F. Kafilzadeh. 1994. The effect upon fermentation and nutritive value of silages produced after treatment by three different inoculants of lactic acid bacteria applied alone or in combination. Grass and Forage Sci. 49:324-333. crossref(new window)

SAS. Institute Inc. 1985. SAS/STAT User`s Guide: Version 6. 4th edn. SAS Institute Inc., Cary, North Carolina.

Seale, D. R. 1986. Bacterial inoculants as silage additives. J. Appl. Bacteriol., Symp. Suppl. 9S-26S.

Smith, D. 1973. The nonstructural carbohydrates. In : Chemistry and Biochemistry of Herbage (Ed. G. W. Butter and R. W. Bailey). Academic press, New York. Volume. 1:105-155.

Weinberg, Z. G., G. Ashbell and G. Azrieli. 1988. The effect of applying lactic acid bacteria at ensilage on the chemical and microbiological composition of vetch, wheat and alfalfa silages. J. Appl. Bacteriol. 64:1-7.

Wylam, C. 1953. Analytical studies on the carbohydrates of grasses and clovers. III. Carbohydrate breakdown during wilting and ensilage. J. Sci. Food Agric. 4:527-531. crossref(new window)