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Effects of Casein and Protein-free Diets on Endogenous Amino Acid Losses in Pigs
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 Title & Authors
Effects of Casein and Protein-free Diets on Endogenous Amino Acid Losses in Pigs
Zhang, Yongcheng; Li, Defa; Fan, Shijun; Piao, Xiangshu; Wang, Jitan; Han, In K.;
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Quantification of endogenous amino acid loss at the terminal ileum is an essential means for calculation of the true amino acid digestibility of a feedstuff. Since nitrogen appeared in the determined diet or not could shift the results very much, also, none of digestibility markers could be recovered with 100% rate at the terminal ileum, the objectives of the present study were: (1) to determine endogenous amino acid losses when fed either a casein diet or a protein-free diet and (2) to examine the reliability of chromic oxide or acid insoluble ash in the protein-free diet. Six ileal-cannulated pigs ( BW) with a simple T-cannula in the terminal ileum were used in a replicated Latin square designed trial, after allowed a 14 d recuperation period. Each test period ran for 12 days comprised of a 10 d adjustment period and a 2 d collection period. The endogenous AA losses of His, Ile, Lys, Cys, Thr, Val, Trp, Asp, Glu, and Ser from pigs fed the casein diet were significantly higher than those of the protein-free diet (p<0.05). No significant difference was found in the amount of endogenous amino acid loss when determined with the different markers in the protein-free diet (p>0.05). These data suggest that endogenous amino acid loss could be underestimated when a protein-free diet is used. A direct effect of dietary peptides on the endogenous amino acid loss was found when the casein diet was fed. Our results also indicate that acid insoluble ash can be used as an inert marker as an alternative to chromic oxide when measuring endogenous amino acid loss.
Protein-Free Diet;Casein;Endogenous Amino Acids;Pig;Digestibility Markers;
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아세아태평양축산학회지, 2014. vol.27. 8, pp.1124-1130 crossref(new window)
Animal models for determining amino acid digestibility in humans – a review, British Journal of Nutrition, 2012, 108, S2, S273  crossref(new windwow)
Use of markers to determine the ileal digestibility of amino acids for swine, Ciência e Agrotecnologia, 2013, 37, 3, 259  crossref(new windwow)
Ileal Endogenous Amino Acid Flow Response to Nitrogen-free Diets with Differing Ratios of Corn Starch to Dextrose in Pigs, Asian-Australasian Journal of Animal Sciences, 2014, 27, 8, 1124  crossref(new windwow)
Effects of fish meal replacement by soybean peptide on growth performance, digestive enzyme activities, and immune responses of yellow catfish Pelteobagrus fulvidraco, Fisheries Science, 2016, 82, 4, 665  crossref(new windwow)
Neutral detergent fiber increases endogenous ileal losses but has no effect on ileal digestibility of amino acids in growing pigs, Animal Science Journal, 2016, 88, 2, 322  crossref(new windwow)
Ileal endogenous losses in pigs feeding a protein-free diet or diets with different contents of casein or crystalline amino acids, Archives of Animal Nutrition, 2017, 71, 3, 210  crossref(new windwow)
AOAC. 1990. Official Methods of Analysis (15th Ed.). Association of Official Analytical Chemists, Arlington, Virginia.

Butts, C. A., P. J. Moughan, W. C. Smith and D. H. Garr. 1993a. Endogenous lysine and other amino acid flows at the terminal ileum of the growing pigs (20 kg body weight): The effect of protein-free, synthetic amino acid, peptide and protein alimentation. J. Sci. Food Agric. 61:31-40. crossref(new window)

Butts, C. A., P. J. Moughan, W. C. Smith, G. W. Reynolds and D. J. Garrick. 1993b. The effects of food dry matter intake on endogenous ileal amino acid excretion determined under peptide alimentation in the 50 kg liveweight pig. J. Sci. Food Agric. 62:235-243. crossref(new window)

Chung, T. K. and D. H. Baker. 1992. Apparent and true amino acid digestibility of a crystalline amino acid mixture and of casein: Comparison of values obtained with ileal-cannulated pigs and cecectomized cockerels. J. Anim. Sci. 70(12):3781-3790.

de Lange, C. F. M., W. B. Souffrant and W. C. Sauer. 1990. Real ileal protein and amino acid digestibilities in feedstuffs for growing pigs as determined with the $^{15}N$-isotope dilution technique. J. Anim. Sci. 68:409-418.

de Lange, C. F. M., W. C. Sauer, R. Mosenthin and W. B. Souffrant. 1989a. The effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and feces in pigs. J. Anim. Sci. 67:746-754.

de Lange, C. F. M., W. C. Sauer and W. B. Souffrant. 1989b. The effect of protein status of the pig on the recovery and amino acid composition of endogenous protein in digesta collected from the distal ileum. J. Anim. Sci. 67:755-762.

Dierick, N., I. Vervaeke, J. Decuypere, H. van der Heyde and H. K. Henderickx. 1983. Influence de la nature et du niveau des fibres brutes sur la digestibilite ileale at fecal apparente de la matiere chez les porcs. Rev. Agric. 36:1961-1970.

Donkoh, A., P. J. Moughan and P. C. H. Morel. 1995. Comparison of methods to determine the endogenous amino acid flow at the terminal ileum of the growing rat. J. Sci. Food Agric. 67:359-366. crossref(new window)

Donkoh, A. and P. J. Moughan. 1999. Endogenous ileal nitrogen and amino acid flows in the growing pig receiving a proteinfree diet and diets containing enzymically hydrolysed casein or graded levels of meat and bone meal. Anim. Sci. 68:511-518. crossref(new window)

Han, In. K. and J. H. Lee. 2000. The role of synthetic amino acids in monogastric animal production. Asian-Aus. J. Anim. Sci. 13(4):543-560. crossref(new window)

Jagger, B., J. Wiseman, D. J. A. Cole and J. Craigon. 1992. Evaluation of inert markers for the determination of ileal and faecal apparent digestibility values in the pigs. Br. J. Nutr. 68:729-739. crossref(new window)

Kavanagh, S., P. B. Lynch, F. O'Mara and P. J. Caffrey. 2001. A comparison of total collection and marker technique for the measurement of apparent digestibility of diets for growing pigs. Anim. Feed Sci. Technol. 89:49-58. crossref(new window)

Low, A. G. 1980. Nutrient absorption in pigs. J. Sci. Food Agric. 31:1087-1130. crossref(new window)

Low, A. G. 1982. Digestibility and availability of amino acids from feedstuffs for pigs. A review. Livest. Prod. Sci. 9:511-520. crossref(new window)

McCarthy C. H., F. X. Aherne and D. B. Okai. 1974. Use of HClinsoluble ash as an index for determining apparent digestibility with pigs. Can. J. Anim. Sci. 54:107-109. crossref(new window)

Millward, D. J., P. J. Garlick and P. J. Reeds. 1976. The energy cost of growth. Proc. Nutr. Soc. 35:339-349. crossref(new window)

Moughan, P. J. and S. M. Rutherfurd. 1990. Endogenous flow of total lysine and other amino acids at the distal ileum of the protein or peptide fed rat: The chemical labeling of gelatin protein by transformation of lysine to homoarginine. J. Sci. Food Agric. 52:179-192. crossref(new window)

National Research Council. 1998. Nutrient Requirement of Swine. 10th Ed. National Academy Press. Washington, DC.

Rodwell, V. W. 1985. Catabolism of the carbon skeletons of amino acids. In: Review of Biochemistry, 20th Ed. (Ed. S. Harper). Los Altos. California: Lange Medical Publications. pp. 124-133.

Sauer, W. C, L. Ozimek. 1986. Digestibility of amino acids in swine: Results and their practical application: A review. Livest. Prod. Sci. 15:367-387. crossref(new window)

Sauer, W. C. and K. de Lange. 1992. Novel methods for determining protein and amino acid digestibilties in feed stuffs. In: Modern Methods in Protein Nutrition and Metabolism (Ed. S. Nissen). Academic Press Inc., San Diego, CA. pp. 87-120.

Souffrant, W. B. 1991. Endogenous nitrogen losses during digestion in pigs. In: Digestive Physiology in Pigs (Ed. M. W. Verstegen, A. J. Huisman and L. A. den Hartog). Wageningen, Netherlands: EAAP Publication. No. 54:147-165.

SPSS for Windows Base System User's Guide Release 6.0. Marija J. Norusis/SPSS Inc.

Zhang, J. H., D. F. Li., X. S. Piao and Y. C. Zhang. 2000. Determination of ileal apparent amino acids digestibility of feather meal with different inert markers. In: Symposium of 6th Animal Nutrition of China. pp. 622-629.

Zhu, X. P., D. F. Li., S. Y. Qiao, C. T. Xiao, Q. Y. Qiao and C. Ji. 1998. Evaluation of HP 300 soybean protein in starter pig diets. Asian-Australian J. Anim. Sci. 11:201-207. crossref(new window)