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Cytoskeletal Patterns, In Vitro Maturation and Parthenogenetic Development of Rabbit GV Oocytes
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 Title & Authors
Cytoskeletal Patterns, In Vitro Maturation and Parthenogenetic Development of Rabbit GV Oocytes
Ju, J.C.; Chen, T.H.; Tseng, J.K.; Tsay, C.; Yeh, S.P.; Chou, P.C.; Chen, C.H.; Liu, C.T.;
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 Abstract
The purposes of this study were to optimize the in vitro maturation (IVM) and culture (IVC) systems of rabbit oocytes. Cytoskeletal structures in the germinal vesicle stage (GV) and during IVM are also investigated. Ovaries were transported from local slaughterhouses and the cumulus-oocyte complexes (COCs) were collected from ovarian follicles (). COCs were randomly allocated to TCM199-based medium (, TCM-199) supplemented with , glucose, sodium pyruvate and FSH (), (), (), or (), for IVM. In Experiment 1, COCs were retrieved from the follicles and 51 GV oocytes were fixed in the fixative (MTSB-XF) for nuclear and cytoplasmic examinations. In Experiment 2, progressive changes of both the nucleus and the cytoskeleton were examined at 0, 6, 16, and 20 h after IVM. Maturation (MR) and developmental rates were assessed in Experiment 3. Cytoplasmic microtubules (MT) were clearly observed in rabbit GV oocytes. To our knowledge, this is the first report that describes the appearance of MT structures in the GV stage ooplasm. Tremendous variations in cytoskeletal alterations were observed among treatments with the exception of the vitelline ring (VR), which is constantly visible and unchanged during maturation. Germinal vesicle breakdown (GVBD) does not occur at 6 h after onset of maturation culture. When the oocytes for IVM were collected within 2 h, results from Experiment 3 showed that rates of nuclear maturation were 42, 8, 42, 37 and 65% at 16 h of IVM for through , respectively, in which , and had significantly greater MR than those in other groups (p<0.05). Morula/blastocyst development after parthenogenetic activation ranged from 20 to 63% with significantly greater rates in , and (p<0.05). These results suggested that oocytes recovered from slaughterhouse ovaries can be matured and parthenogenetically activated in vitro, but the MR remained low in this study. Addition of and LH in the medium may be beneficial for cytoplasmic maturation, but FBS exerts a nega- tive role in the subsequent development of parthenogenetic embryos when energy substrates are provided in the IVC media. More studies are required for improving the MR and further development of the GV stage rabbit oocytes.
 Keywords
Germinal Vesicle;Cytoplasmic Microtubule;In Vitro Maturation;Cytoskeleton;Rabbit;
 Language
English
 Cited by
 References
1.
Abeydeera, L. R., W. H. Wang, T. C. Cantley, A. Rieke, R. S. Prather and B. N. Day. 1998. Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization. Mol. Reprod. Dev. .51:395-401. crossref(new window)

2.
Albertini, D. F. and J. T. Clark. 1981. Visualization of assembled and disassembled microtubule protein by double fluorescence microscopy. Cell. Biol. Intl. Reports 5(4):387-397. crossref(new window)

3.
Aman, R. B. and J. E. Parks.1994. Effect of cooling and rewarming on the meiotic spindle and chromosomes of in vitro-matured bovine oocytes. Biol. Reprod. 50:103-110. crossref(new window)

4.
Cha, K. Y., J. J. Koo, J. J. Ko, D. H. Choi, S. Y. Han and T. K. Yoon. 1991. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil. Steril. 55:109-113.

5.
Cheng, S. P., Y. C. Chang, J. C. Ju and C. L. Young. 1988. In vitro development of intact and micromanipulated rabbit embryos. Proceedings of the three-way faculty exchange seminar on the application of biotechnology to agriculture. Seoul, Korea, pp. 235-244.

6.
Collas, P., J. J. Balise and J. M. Robl. 1992. Influence of cell cycle stage of the donor nucleus on development of nuclear transplant rabbit embryos. Biol. Reprod. 46: 492-500. crossref(new window)

7.
Edwards, R. G. 1965. Maturation in vitro of mouse , sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature 208: 349-351. crossref(new window)

8.
Du, F., J. R. Giles, R. H. Foote, K. H. Graves, X. Yang and R. W. Moreadith. 1995. Nuclear transfer of putative rabbit embryonic stem cells leads to normal blastocyst development. J. Reprod. Fertil. 104, 219-223. crossref(new window)

9.
Hahn, J. 1984. The value of laboratory animal models in embryo transfer research. Theriogenology. 21:45-59. crossref(new window)

10.
Hafez, E. S. E. and B. Hafez. 2000. Folliculogenesis, egg, maturation and ovulation. In: 'Reproduction in Farm Animals', (Ed. E. S. E. Hafez and B. Hafez). 7th edition, Lippincott Williams and Wilkins, Philadelphia, USA. pp. 68-81.

11.
Haper, K. M. and B. G. Brackette. 1993. Bovine blastocyst development after in vitro maturation in a defined medium with epidermal growth factor and low concentrations of gonadotropins. Biol. Reprod. 48:409-416. crossref(new window)

12.
Jelinkova, L, M. Kubelka, J. Motlik and P. Guerrier. 1994. Chromatin condensation and histone H1 kinase activity during growth and maturation of rabbit oocytes. Mol. Reprod. Dev. 37: 210-215. crossref(new window)

13.
Ju, J. C., C. Tsay, C. W. Ruan and X. Yang. 2001. Reversibility in the cytoskeleton and development of pig oocytes treated with roscovitine. Biol Reprod. 64:38(Abstr.).

14.
Ju, J. C., J. E. Parks and X. Yang. 1999. Thermotolerance of IVMderived bovine oocytes and embryos after short-term heat shock. Mol. Reprod. Dev. 53:336-340. crossref(new window)

15.
Kim, N. H., K. S. Chung and B. N. Day. 1997. The distribution and requirements of microtubules and microfilaments during fertilization and parthenogenesis in pig oocytes. J. Reprod. Fertil. 111:143-149. crossref(new window)

16.
Kim, N. H., H. Funahashi, R. Prather, G. Schatten and B. N. Day. 1996a. Microtubule and microfilament dynamics in porcine oocytes during meiotic maturation. Mol. Reprod. Dev. 43:248-255. crossref(new window)

17.
Kim, N. H., C. Simerly, H. Funahashi, G. Schatten,, B. N. Day. 1996b. Microtubule organization in porcine oocytes during fertilization and parthenogenesis. Biol. Reprod. 54:1397-1404. crossref(new window)

18.
Lalantha, R., L. R. Abeydeera, W. H. Wang, T. C. Cantley, A. Rieke, R. S. Prather and B. N. Day. 1998. Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization. Mol. Reprod. 51(4): 395-401. crossref(new window)

19.
Leibfried-Rutledge, M. L., H. M. Florman and N. L. First. 1989. The molecular biology of mammalian oocyte maturation. In: 'The Molecular Biology of Fertilization', (Ed. H. Schatten and G. Schatten). Academic Press Inc., San Diego, CA 92101. pp. 259-301.

20.
Liu, L., J. C. Ju and X. Yang. 1998. Parthenogenetic development and protein pattern of newly matured bovine oocytes after chemical activation. Mol. Reprod. Dev. 49:298-307. crossref(new window)

21.
Liu, C. T., C. H. Chen, S. P. Cheng and J. C. Ju. 2002. Parthenogenesis of rabbit oocytes activated by different stimuli. Anim. Reprod. Sci. 70:267-276. crossref(new window)

22.
Lorenzo, P. L., M. J. Illera, J. C. Illera and M. Illera.1994. Enhancement of cumulus expansion and nuclear maturation during bovine oocyte maturation in vitro by the addition of epidermal growth factor and insulin-like growth factor I. J. Reprod. Fertil. 101:697-701. crossref(new window)

23.
Lorenzo, P. L., P. G. Rebollar, M. J. Illera, J. C. Illera, M. Illera and J. M. R. Alvarino. 1996. Stimulatory effect of insulinlike growth f actor I and epidermal growth factor on the maturation of rabbit oocytes in vitro. J. Reprod. Fertil.. 107:109-117. crossref(new window)

24.
Lorenzo, P. L., J. C. Illera, G. Silvan, C. J. Munro, M. J. Illera and M. Illera. 1997. Steroid level response to insulin-like growth factor-I in oocytes matured in vitro. J. Reprod. Immunol. 35: 11-29. crossref(new window)

25.
McDonald, L. E. 1980. Introduction. In: 'Veterinary Endocrinology and Reproduction', 3rd ed., (Ed. L. E. McDonald. Lea and Febiger). Philadelphia. pp.1-12.

26.
McGaughey, R. W., D. H. Montgomery and J. D. Richter. 1979. Germinal vesicle configurations and patterns of polypeptide synthesis of porcine oocytes from antral follicles of different size as related to their competency for spontaneous maturation. J. Exp. Zool. 209:239-254. crossref(new window)

27.
Motlik, J. and J. Fulka. 1986. Factors affecting meiotic competence in pig oocytes. Theriogenology 25:87-96. crossref(new window)

28.
Motlik, J., J. Fulka Jr, R. Prochazka, Z. Rimkevicova, M. Kubelka and J. Fulka. 1989. RNA and protein synthesis requirements for the resumption of meiosis in rabbit oocytes: The role of cumulus cells. Reprod. Nutr. Dev. 29:601-609. crossref(new window)

29.
Ocampo, M. B., T. C. Lerma and H. Kanagawa. 1991. Timing of sequential changes in chromosome configurations during the 1st meiotic division of pig oocytes cultured in vitro. Jpn. J. Vet. Res. 38:127-137.

30.
Ozil, J. P. 1990. The parthenogenetic development of rabbit oocytes after repetitive pulsatile electrical stimulation. Development 109:117-128.

31.
Piotrowska, K., J. A. Modlinski, M. Korwin-Kossakowski and J. Karasiewicz. 2000. Effects of preactivation of ooplasts or synchronization of blastomere nuclei in G1 on preimplantation development of rabbit serial nuclear transfer embryos. Biol. Reprod. 63:677-682. crossref(new window)

32.
Plancha, C. E. and D. F. Albertini. 1994. Hormonal regulation of meiotic maturation in the hamster oocyte involves a cytoskeleton-mediated process. Biol. Reprod. 51:852-864. crossref(new window)

33.
Reed, M. L., J. L. Estrada, M. J. Illera and R. M. Petters. 1993. Effects of epidermal growth factor, insulin-like growth factor-1, and dialyzed porcine follicular fluid on porcine oocyte nuclear maturation in vitro. J. Exp. Zool. 266:74-78. crossref(new window)

34.
Sutovsky, P., L. Jelinkova, L. Antalikova and J. Motlik. 1993. Ultrastructural cytochemistry of the nucleus and nucleolus in growing rabbit oocytes. Biol. Cell 77:173-180. crossref(new window)

35.
Suzuki, H., X. Yang and R. H. Foote. 1994. Surface alterations of the bovine oocyte and its investments during and after maturation and fertilization in vitro. Mol. Reprod. Dev. 38:421-430. crossref(new window)

36.
Vanderhyden, B. C. and D. T. Armstrong. 1990. Effect of gonadotropins and granulosa cells secretions on the maturation and fertilization of rat oocytes in vitro. Mol. Reprod. Dev. 26:337-346. crossref(new window)

37.
Wu, B., J. Tong and S. P. Leibo. 1998. Effect of chilling bovine germinal vesicle-stage oocytes on formation of microtubules and the meiotic spindle. Theriogenology 49:177. crossref(new window)

38.
Yang, X., Y. Chen and R. H. Foote. 1990. Potential of hypertonic medium treatment for embryo micromanipulation: I. Survival of rabbit embryos in vitro and in vivo following sucrose treatment. Mol. Reprod. Dev. 27:110-117. crossref(new window)

39.
Yang, X., S. Jiang, A. Kovacs and R. H. Foote. 1992. Nuclear totipotency of cultured rabbit morulae to support full-term development following nuclear transfer. Biol. Reprod. 47:636-643. crossref(new window)