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Plasma Amino Acid Status of Crossbred Heifers Fed Two Levels of Dietary Protein and its Relationship to Puberty Onset
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Plasma Amino Acid Status of Crossbred Heifers Fed Two Levels of Dietary Protein and its Relationship to Puberty Onset
Swain, R.K.; Kaur, Harjit;
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Twelve prepubertal Karan Fries heifers (15 months, ) were divided into two equal groups. Group 1 was fed as per NRC requirements and group 2 was fed 20% more protein than group 1 heifers. The experimental feeding was continued until the onset of puberty in both the groups. Blood samples were collected at fortnightly intervals and analyzed for amino acids using HPLC. Group 1 and 2 heifers required and days of experimental feeding to exhibit first estrus resulting in total age at puberty as and days in the two groups respectively. The concentration of total amino acids averaged 4.40 and 4.89 mmol/l and those of non-essential amino acids (NEAA) was 2.32 and 2.49 mmol/l in groups 1 and 2, respectively. The concentration of plasma essential amino acids i.e. histidine, threonine, valine, methionine, isoleucine, leucine and phenylalanine were higher (p<0.01) in group 2 than group 1. Plasma concentration of large neutral amino acids (LNAA) was significantly higher in group 2 (1.28 mmol/l) than in group 1 (1.12 mmol/l). Increased levels of leucine, isoleucine and valine are implicated in increased follicular growth and development in prepubertal heifers and resulted in a 26 day earlier attainment of puberty by 26 days in an experimental period of six months in group 2 heifers. Increased concentrations of aspartate and tyrosine in group 2 heifers might be associated with the release of GnRH from the hypothalamus influencing LH release from anterior pituitary in such animals. It is therefore evident that increased availability of certain amino acids in heifers fed high protein diet might have led to early onset of puberty.
Amino acids;Protein;Puberty;Heifers;Tyrosine;Aspartate;
 Cited by
Acworth, I. N., M. J. During and R. J. Wurtman. 1988. Tyrosine: effects on catecholamine release. Brain Res. Bull. 21:473. crossref(new window)

Copyright Millipore Corporation, 1987. Liquid chromatographic analysis of amino acids in feeds and foods using a modification of the PICO-TAG method revision. Waters instruments (India) Pvt. Ltd., 301-302, Oriental House, Community Center, Gulmohar Enclave, New Delhi.

Deligeorgis, S. G., S. Chadio and J. Menegatos. 1996. Pituitary responsiveness to GnRH in lambs undernourished during fetal life. Anim. Reprod. Sci. 43:113-121. crossref(new window)

Downing, J. A. 1994. Interaction of nutrition and ovulation rate in ewes. Ph. D. thesis, Macquarie University, North Ryde, NSW, Australia.

Downing, J. A., J. Joss and R. J. Scaramuzzi. 1995. A mixture of the branched chain amino acids leucine, isoleucine and valine increases ovulation rate in ewes when infused during the luteal phase of the oestrous cycle: An effect that may be mediated by insulin. J. Endocrinol. 145:315. crossref(new window)

Downing, J. A., J. Joss and R. J. Scaramuzzi. 1996. The effects of n-methyl–D,L-aspartic acid and aspartic acid on the plasma concentration of gonadotrophins, GH and prolactin in the ewe. J. Endocrinol. 149:65-72. crossref(new window)

Ferguson, J. D., T. L. Blanchard, D. T. Galligan, D.C. Hoshall and Chlupa, W. 1988. Infertility in dairy cattle fed a high percentage of protein degradable in the rumen. J. Am. Vet. Med. Assoc. 192:659-661.

Hall, J. B., K. K. Schillo, S. M. Hileman and J. A. Boling. 1992. Does tyrosine act as a nutritional signal mediating the effects of increased feed intake on luetinizing hormone patterns in growth-restricted lambs? Biol. Reprod. 46:573. crossref(new window)

Hall, J. B., R. B. Staigmiller, R. A. Belows, R. E. Short, W. M. Mosley and S. E. Bellows. 1995. Body composition and metabolic profiles associated with puberty in beef heifers. J. Anim. Sci. 73:3409-3420.

Hammerl, J. and M. Russe.1987. Der einflub der aminosaure Ltyrosine and L-tryptophan auf das frucht barkeitsgeschehen bei Jungen ratten. Zuchthyg, 2280.

Jordan, E. R., T. E. Chapman, D. W. Holtan and L. V. Swanson. 1983. Relationship of dietary crude protein to composition of uterine secretions and blood in high-producing postpartum dairy cows. J. Dairy Sci. 66:1854-1862.

Kaur, H. and S. P. Arora. 1989. Growth and puberty as influenced by plane of nutrition in Murrah buffaloes. Buffalo J. 1:57-63.

Kaur, H and S. P. Arora. 1995. Dietary effects on ruminant livestock reproduction with particular reference to protein. Nutr. Res. Rev. 8:121-136. crossref(new window)

Lohakare J, S. A. Khan and A. K. Pattanaik. 2001. Growth performance of crossbred calves fed three different levels of dietary protein. Proceedings Xth Anim. Nutrition Conference, NDRI, Karnal, Abstr. No. 195.

National Research Council. 1989. Nutrient Requirements of Dairy Cattle. 6th Edn. National Academy of Sciences, Washington, DC.

Patil, N. V. 1993. Dietary influence of rumen degradable protein on blood metabolites and some hormones in cyclic and pregnant goats. Ph.D. thesis, NDRI Deemed University, Karnal, India.

Ranjhan S. K. 1980. Animal Nutrition in Tropics. Vikas Publishing House Pvt. Ltd. Delhi, India.

Remirez, V. D., K. Kim and D. Dluzen. 1985. Progesterone action on the LHRH and the nigrostriatal dopamine neuronal systems in vitro and in vivo studies. Recent Prog. Horm. Res.41:421.

Riis, P.M. 1983. The pools of tissue constituents and products: proteins. World Anim. Sci. 3:75.

Schillo, K. K. 1992. Effects of dietary energy on control of luteinizing hormone secretion in cattle and sheep. J. Anim. Sci. 70:1271-1282.

Schillo, K. K., J. B. Hall and S. M. Hileman. 1992. Effects of nutrition and season on the onset of puberty in the beef heifer. J. Anim. Sci. 70:3994-4005.

Shahab, M., K. D. Nusser, J. L. Peters and D. R. Deaver. 1995. Involvement of N methyl-D-aspartate receptor using excitatory amino acid neurotransmitters in control of pulsatile secretion of LH during sexual development in Holstein bull calves. J. Reprod. Fertil. 105:77. crossref(new window)

Smith, J. F., C. J. Clark, J. Parr and J. Konlechner. 1992. Effect of intravenous infusion of branched chain amino acids (BCAA) or insulin on ovulation rate and plasma levels of insulin and BCAA in ewes. Proc. Aust. Soc. Reprod. Biol., 24, Abstr. No. 30.

Snedecor, G. W. and W. G. Cochran. 1980. Statistical Methods. 6th edn. Oxford and IBH Publishing Co., Calcutta.

Sniffen, C. J. and D. R. Jacobson. 1975. Net amino acid absorption in steers fed alfalfa hay cut at two stages of maturity. J. Dairy Sci. 58:371-385.

Swain, R. 1996. Influence of dietary protein on growth, prepubertal luteinizing hormone secretion and onset of puberty in crossbred heifers. Ph.D. Thesis, NDRI Deemed University, Karnal, India.