Advanced SearchSearch Tips
Distribution and Activities of Hydrolytic Enzymes in the Rumen Compartments of Hereford Bulls Fed Alfalfa Based Diet
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Distribution and Activities of Hydrolytic Enzymes in the Rumen Compartments of Hereford Bulls Fed Alfalfa Based Diet
Lee, S.S.; Kim, C.-H.; Ha, J.K.; Moon, Y.H.; Choi, N.J.; Cheng, K.-J.;
  PDF(new window)
The distribution and activities of hydrolytic enzymes (cellulolyti, hemicellulolytic,pectinolytic and others) in the rumen compartments of Hereford bulls fed 100% alfalfa hay based diets were evaluated. The alfalfa proportion in the diet was gradually increased for two weeks. Whole rumen contents were processed into four fractions: Rumen contents including both the liquid and solid fractions were homogenized and centrifuged, and the supernatant was assayed for enzymes located in whole rumen contents (WRE); rumen contents were centrifuged and the supernatant was assayed for enzymes located in rumen fluids (RFE); feed particles in rumen contents were separated manually, washed with buffer, resuspended in an equal volume of buffer, homogenized and centrifuged and supernatant was assayed for enzymes associated with feed particles (FAE); and rumen microbial cell fraction was separated by centrifugation, suspended in an equal volume of buffer, sonicated and centrifuged, and the supernatant was assayed for enzymes bound with microbial cells (CBE). It was found that polysaccharide-degrading proteins such as -1,4-D-endoglucanase, -1,4-D-exoglucanase, xylanase and pectinase enzymes were located mainly with the cell bound (CBE) fraction. However, -D-glucosidase, -D-fucosidase, acetylesterase, and -L-arabinofuranosidase were located in the rumen fluids (RFE) fraction. Protease activity distributions were 37.7, 22.1 and 40.2%, and amylase activity distributions were 51.6, 18.2 and 30.2% for the RFE, FAE and CBE fractions, respectively. These results indicated that protease is located mainly in rumen fluid and with microbial cells, whereas amylase was located mainly in the rumen fluid.
Rumen;Hydrolytic Enzyme;Polysaccharidases;Glycosidases;Enzyme Distributions;
 Cited by
Influences of Surfactant Tween 80 on the Gas Production, Cellulose Digestion and Enzyme Activities by Mixed Rumen Microorganisms,;;

아세아태평양축산학회지, 2003. vol.16. 8, pp.1151-1157 crossref(new window)
L., Aquaculture Research, 2016, 48, 7, 3801  crossref(new windwow)
Exploring the bioprospecting and biotechnological potential of white-rot and anaerobic Neocallimastigomycota fungi: peptidases, esterases, and lignocellulolytic enzymes, Applied Microbiology and Biotechnology, 2017, 101, 8, 3089  crossref(new windwow)
Ali, B. R. S., L. Zhou, F. M. Graves, R. B. Freedman, G. W. Black, H. J. Gilbert and G. P. Hazlewood. 1995. Cellulases and hemicellulases of the anaerobic fungus Piromyces constitute a multiprotein cellulose-binding complex and are encoded by multigene families. FEMS Microbiol. Lett. 125:15-22. crossref(new window)

Attwood, G. T. and K. Reilly. 1995. Identification of proteolytic rumen bacteria isolated from New Zealand cattle. J. Appl. Bacteriol. 79:22-29.

Cheng, K. J. and T. A. McAllister. 1997. Compartmentation in the rumen. In: The Rumen Microbial Ecosystem (Ed. P. N. Hobson and C. S. Stewart). Elsevier Applied Science, London. pp. 492-522.

Chesson, A. and C. W. Forsberg. 1988. Polysaccharide degradation by rumen microorganisms. In: The Rumen Microbial Ecosystem (Ed. P. N. Hobson and C. S. Stewart). Elsevier Applied Science, London. pp. 329-381

Chesson, A., C. S. Stewart, K. Dalgarno and T. P. King. 1986. Degradation of isolated grass mesophyll, epidermis and fibre cell walls in the rumen and by cellulolytic rumen bacteria in axenic culture. J. Appl. Bacteriol. 60:327-336.

Coleman, G. S. 1986. The amylase activity of 14 species of entodiniomorphid protozoa and the distribution of amylase in digesta fractions of sheep containing no protozoa or one of seven different protozoal populations. J. Agric. Sci. 107:709-721. crossref(new window)

Craig, W. M., D. R. Brown, G. A. Broderick and D. B. Ricker. 1987a. Post-prandial compositional changes of fluid- and particle-associated ruminal microorganisms. J. Anim. Sci. 65:1042-1048.

Craig, W. M., G. A. Broderick and D. B. Ricker. 1987b. Quantitation of microorganisms associated with the particulate phase of ruminal ingesta. J. Nutr. 117:56-62.

Doerner, K. C. and B. A. White. 1990. Assessment of the endo- - 1,4-glucanase components of Ruminococcus flavefaciens FD-1. Appl. Environ. Microbiol. 56:1844-1850.

Flint, H. J., J. X. Zhang and J. Martin. 1994. Multiplicity and expression of xylanase in the rumen cellulolytic bacterium Ruminococcus flavefaciens. Curr. Microbiol. 29:139-143. crossref(new window)

Forsberg, C. W. and L. Lam. 1977. Use of adenosine 5'- triphosphate as an indicator of the microbiota biomass in bovine rumen contents. Appl. Environ. Microbiol. 33: 528-537.

Forsberg, C. W., J. J. Beveridge and A. H. Hellstrom. 1981. Cellulase and xylanase release from Bacteroides succinogenes and its importance in the rumen environment. Appl. Environ. Microbiol. 42:886-896.

Gilbert, H. J., G. P. Hazlewood, J. L. Laurie, C. G. Orpin and G. P. Xue. 1992. Homolytic catalytic domains in rumen fungal xylanase: homologous catalytic domains in a fungal xylanase: evidence for gene duplication and prokaryotic origin. Mol. Microbiol. 6:2065-2072. crossref(new window)

Gong, C. S. and G. T. Tsao. 1979. Cellulase biosynthesis and regulation. Ann. Rep. Ferm. Proc. 3:111-140.

Grouleau, D. and C. W. Forsberg. 1981. Cellulolytic activity of the rumen bacterium Bacteroides succinogenes. Can. J. Microbiol. 29:504-517.

Leatherwood, J. M. 1965. Cellulase from Ruminococcus albus and mixed rumen microorganisms. Appl. Microbiol. 13:771-775.

Lee, S. S., J. K. Ha and Y. J. Choi. 1995. Studies on the isolation and identification of rumen fungi, characterization of cellulolytic fungal enzymes, and its industrial utilization: V. Cellulolytic enzyme assessments of ruminal fungi isolated from the rumen of Korean native cattle and goat. Kor. J. Anim. Feed. 19(6):507-524.

Lee, S. S., K. J. Shin, W. Y. Kim, J. K. Ha and I. K. Han. 1999. The rumen ecosystem : As a fountain source of nobel enzymes. Asian-Aus. J. Anim. Sci. 12(6):988-1001.

Legay-Carmier, F and D. Bauchart. 1989. Distribution of bacteria in the rumen contents of dairy cows given a diet supplemented with soya-bean oil. Brit. J. Nutr. 61:725-740. crossref(new window)

Lever, M. 1977. Carbohydrate determination with 4-hydroxybenzoic acid hydrazide (PAHBAH): effect of bismuth on the reaction. Anal. Biochem. 81:21-27. crossref(new window)

Malburg, L. M. and C. W. Forsberg. 1993. Fibrobacter succinogenes possesses at least nine distinct glucanase genes. Can. J. Microbiol. 39:882-891.

Martin, C. and B. Michalet-Doreau. 1995. Variations in mass and enzyme activity of rumen microorganisms: effect of barley and buffer supplements. J. Sci. Food Agric. 67:407-413. crossref(new window)

Merry, R. J. and A. B. McAllan. 1983. A comparison of the chemical composition of mixed bacteria harvested from the liquid and solid fractions of rumen digesta. Br. J. Nutr. 50:701-709. crossref(new window)

Michel, V., G. Fonty, L. Millet, F. Bonnemoy and P. Gouet. 1993. In vitro study of the proteolytic activity of rumen anaerobic fungi. FEMS Microbiol. Lett. 110:5-9. crossref(new window)

Nelson, N. 1944. A photometric adaptation of the Somogyi method for the determination of glucose. J. Biologic. Chem. 54:2675-2680.

Pettipher, G. L. and M. J. Latham. 1979a. Characteristics of enzymes produced by Ruminococcus flavefaciens rumen bacteria which degrade plant cell walls. J. Gen. Microbiol. 110:21-27.

Pettipher, G. L. and M. J. Latham. 1979b. Production of enzymes degrading plant cell walls and fermentation of cellobiose by Ruminococcus flavefaciens in batch and continuous culture source of energy for ruminant herbivores. J. Gen. Microbiol. 110:29-38.

Somogyi, M. 1952. Notes of sugar determination. J. Biologic. Chem. 195:19-23.

Tagawa, K. and A. Kaji. 1988. Polygalacturonase from Corticium rofsii. In: Methods in Enzymology (Ed. W. A. Wood and S. T. Kellogg). Academic Press, San Diego. pp. 361-365.

Trinci, A. P. J., D. R. Davies, K. Gull, M. L. Lawrence, B. B. Nielsen, A. Rickers and M. K. Theodorou. 1994. Anaerobic fungi in herbivorous animals. Mycol. Res. 98:129-152. crossref(new window)

Wallace, R. J., R. Onodera and M. A. Cotta. 1988. Metabolism of nitrogen-containing compounds. In: The Rumen Microbial Ecosystem. (Ed. P. N. Hobson and C. S. Stewart). Elsevier Applied Science, London. pp. 283-328

Wells, J. E. and J. B. Russell. 1996. Why do many ruminal bacteria die and lyse so quickly? J. Dairy Sci. 79:1487-1495.

Williams, A. G. and N. H. Strachan. 1984. The distribution of polysaccharide-degrading enzymes in the bovine rumen digesta ecosystem. Curr. Microbiol. 10:215-220. crossref(new window)

Willams, A. G., I. M. Morrison, N. H. Strachan and R. E. Brice. 1984. Distribution of glycoside hydrolases and polysaccharide depolymerases associated with the particulate fraction from the bovine rumen and an artificial rumen. Appl. Biochem. Biotechnol. 9:419-420. crossref(new window)

Yazdi, M. T., J. R. Woodward and A. Radford. 1990. The cellulase complex of Neurospora crassa: activity, stability and release. J. Gen. Microbiol. 136:1313-1319.

중복. 삭제 바람