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Response of Muscle Protein Synthesis to the Infusion of Insulin-like Growth Factor-I and Fasting in Young Chickens
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 Title & Authors
Response of Muscle Protein Synthesis to the Infusion of Insulin-like Growth Factor-I and Fasting in Young Chickens
Kita, K.; Shibata, T.; Aman Yaman, M.; Nagao, K.; Okumura, J.;
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In order to elucidate the physiological function of circulating IGF-I on muscle protein synthesis in the chicken under malnutritional conditions, we administrated recombinant chicken IGF-I using a osmotic mini pump to fasted young chickens and measured the rate of muscle protein synthesis and plasma metabolite. The pumps delivered IGF-I at the rate of . Fractional rate of protein synthesis in the muscle was measured using a large dose injection of L-[]phenylalanine. Constant infusion of chicken IGF-I did not affect plasma glucose level. Significant interaction between dietary treatment and IGF-I infusion was observed in plasma NEFA and total cholesterol concentrations. When chicks were fasted, IGF-I infusion decreased plasma NEFA and total cholesterol concentrations. On the other hand, IGF-I administration did not affect plasma levels of both metabolites. Fasting reduced plasma triglyceride concentration significantly. IGF-I infusion also decreased the level of plasma triglyceride. Plasma IGF-I concentration of young chickens was halved by fasting for 1 d. IGF-I infusion using an osmotic minipump for 1 d increased plasma IGF-I concentration in fasted chicks to the level of fed chicks. Fasting decreased body weight and the loss of body weight was significantly ameliorated by IGF-I infusion. There was a significant interaction between dietary treatment and IGF-I infusion in the fractional rate of breast muscle protein synthesis. There was no effect of IGF-I infusion on muscle protein synthesis in fed chicks. Muscle protein synthesis reduced by fasting was ameliorated by IGF-I infusion, but did not reach to the level of fed control. Muscle weight of fasted chicks infused with IGF-I was similar to fasted birds without IGF-I infusion, which suggests that muscle protein degradation would be increased by IGF-I infusion as well as protein synthesis in fasted chicks.
IGF-I infusion;Muscle;Protein Synthesis;Chickens;
 Cited by
Investigation of the Insulin-like Growth Factor System in Breast Muscle during Embryonic and Postnatal Development in Langshan and Arbor Acres Chickens Subjected to Different Feeding Regimens,;;;;

아세아태평양축산학회지, 2009. vol.22. 4, pp.471-482 crossref(new window)
Ballard, F. J., R. J. Johnson, P. C. Owens, G. L. Francis, F. Z. Upton, J. P. McMurtry and J. C. Wallace. 1990. Chicken insulin-like growth factor-I: Amino acid sequence, radioimmunoassay, and plasma levels between strains and during growth. Gen. Comp. Endocrinol. 79:459-468. crossref(new window)

Bark, T. H., M. A. McNurlan, C. H. Lang and P. J. Garlick. 1998. Increased protein synthesis after acute IGF-I or insulin infusion is localized to muscle in mice. Am. J. Physiol. 275:E118-E123.

Clemmons, D. R., A. Smith-Banks and L. E. Underwood. 1992. Reversal of diet-induced catabolism by infusion of recombinant insulin-like growth factor-I in humans. J. Clin. Endocrinol. Metab. 75:234-238. crossref(new window)

Czerwinski, S. M., J. M. Cate, G. Francis, F. Tomas, D. M. Brocht and J. P. McMurtry. 1998. The effect of insulin-like growth factor-I (IGF-I) on protein turnover in the meat-type chicken (Gallus domesticus). Comp. Biochem. Physiol. 119C:75-80.

Garlick, P. J., M. A. McNurlan and V. R. Preedy. 1980. A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of $[^3H]$phenylalanine. Biochem. J. 192:719-723.

Huybrechts, L. M., E. Decuypere, J. Buyse, E. R. Kuhn and M. Tixier-Boichard. 1992. Effect of recombinant human insulinlike growth factor-I on weight gain, fat content, and hormonal parameters in broiler chickens. Poult. Sci. 71:181-187.

Jacob, R., E. Barrett, G. Plewe, K. D. Fagin and R. S. Sherwin. 1989. Acute effects of insulin-like growth factor I on glucose and amino acid metabolism in the awake fasted rat. Comparison with insulin. J. Clin. Invest. 83:1717-1723. crossref(new window)

Jacob, B., X. Hu, D. Niederstock, S. Hasan, P. H. McNulty, R. S. Sherwin and L. H. Young. 1996. IGF-I stimulation of muscle protein synthesis in the awake rat: permissive role of insulin and amino acids. Am. J. Physiol. 270:E60-E66.

Kerr, D., W. V. Tamborlane, F. Rife and R. S. Sherwin. 1993. Effect of insulin-like growth factor-1 on the responses to and recognition of hypoglycemia in humans. A comparison with insulin. J. Clin. Invest. 91:141-147. crossref(new window)

Kita, K., J. Iwata, T. Shibata, M. A. Conlon and J. Okumura. 1997. Influence of fasting on plasma insulin-like growth factor-I concentration in chickens at early stages of growth. Jpn. Poult. Sci. 34:410-413.

Kita, K., S. Matsunami and J. Okumura. 1996a. Relationship of protein synthesis to mRNA levels in the muscle of chicks under various nutritional conditions. J. Nutr. 126:1827-1832.

Kita, K., T. Muramatsu and J. Okumura. 1993. Effect of dietary protein and energy intakes on whole-body protein turnover and its contribution to heat production in chicks. Br. J. Nutr. 69:681-688. crossref(new window)

Kita, K. and J. Okumura. 1999. Dietary protein levels alter plasma insulin-like growth factor-I concentration of chicks. Jpn. Poult. Sci. 36:25-30.

Kita, K., F. M. Tomas, P. C. Owens, S. E. Knowles, B. E. Forbes, Z. Upton, R. Hughes and F. J. Ballard. 1996b. Influence of nutrition on hepatic IGF-I mRNA levels and plasma concentrations of IGF-I and IGF-II in meat-type chickens. J. Endocrinol. 149:181-190. crossref(new window)

Kovacs, G. T., S. Worgall, P. Schwalbach, T. Steichele, O. Mehls and L. Rosivall. 1999. Hypoglycemic effects of insulin-like growth factor-1 in experimental uremia: can concomitant growth hormone administration prevent this effect?. Horm. Res. 51:193-200. crossref(new window)

Aman Yaman, M., K. Kita and J. Okumura. 2000. Various macronutrient intakes additively stimulate protein synthesis in liver and muscle of food-deprived chicks. J. Nutr. 130:70-76.

McGuinness, M. C. and L. A. Cogburn. 1991. Response of young broiler chickens to chronic injection of recombinant-derived human insulin-like growth factor-I. Domest. Anim. Endocrinol. 8:611-620. crossref(new window)

Read, L. C., F. J. Ballard, G. L. Francis, R. C. Baxter, C. J. Bagley and J. C. Wallace. 1986. Comparative binding of bovine, human and rat insulin-like growth factors to membrane receptors and to antibodies against human insulin-like growth factor-I. Biochem. J. 233:215-221.

Rosebrough, R. W. and J. P. McMurtry. 1993. Protein and energy relationship in the broiler chicken. 11. Effects of protein quantity and quality on metabolism. Br. J. Nutr. 70:667-678. crossref(new window)

Rosebrough, R. W., A. D. Mitchell and J. P. McMurtry. 1996. Dietary crude protein changes rapidly alter metabolism and plasma insulin-like growth factor I concentrations in broiler chickens. J. Nutr. 126:2888-2898.

Schalch, D. S., H. Yang, D. M. Ney and R. D. DiMarchi. 1989. Infusion of human insulin-like growth factor I (IGF-I) into malnourished rats reduces hepatic IGF-I mRNA abundance. Biochem. Biophys. Res. Comm.160:795-800. crossref(new window)

Takano, K., N. Hizuka, K. Shizume, K. Asakawa, I. Fukuda and H. Demura. 1991. Repeated sc administration of recombinant human insulin-like growth factor I (IGF-I) to human subjects for 7 days. Growth Reg. 1:23-28.

Tixier-Boichard, M., L. M. Huybrechts, E. Decuypere, E. R. Kuhn, J. L. Monvoisin, G. Coquerelle, J. Charrier and J. Simon. 1992. Effects of insulin-like growth factor-I (IGF-I) infusion and dietary tri-iodothyronine (T3) supplementation on growth, body composition and plasma hormone levels in sex-linked dwarf mutant and normal chickens. J. Endocrinol. 133:101-110. crossref(new window)

Waterlow, J. C., P. J. Garlick and D. J. Millward. 1978. Protein turnover in the whole body and tissues. In: Protein turnover in mammalian tissues and in the whole body, North-Holland Amsterdam, pp. 626-695.