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Effects of Dietary Fructooligosaccharide on Digestive Enzyme Activities, Intestinal Microflora and Morphology of Growing Pigs
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 Title & Authors
Effects of Dietary Fructooligosaccharide on Digestive Enzyme Activities, Intestinal Microflora and Morphology of Growing Pigs
Xu, Z.R.; Zou, X.T.; Hu, C.H.; Xia, M.S.; Zhan, X.A.; Wang, M.Q.;
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One hundred and twenty-eight growing barrows (Jiaxing BlackDurocLandrace) at an average BW of 20.8 kg were allocated to four treatments for 42 days, each of which was replicated four times with eight pigs per replicate and used to investigate the effects of fructooligosaccharide (FOS) on digestive enzyme activities, intestinal microflora and morphology of growing pigs. The pigs received the same basal corn-soybean meal diet and FOS was added to the basal diet at 0, 2, 4, 6 g/kg diet at the expense of corn, respectively. As compared to control, supplementation with 4 and 6 g/kg FOS significantly improved average daily gain and feed efficiency. Addition of FOS enhanced the growth of Bifidobacterium and Lactobacillus, but inhibited Clostridium and Escherichia coli in the small intestinal and proximal colonic contents. Supplementation with 4 and 6 g/kg FOS significantly improved the activities of total protease, trypsin and amylase in the small intestinal contents. However, FOS had no significant effect on the activity of lipase in the small intestinal contents as well as the digestive enzymes in pancreas. Morphological measurement of jejunal mucosa did show response to consumption of FOS. Villus height and the villus height to crypt depth ratio at the jejunal mucosa were significantly higher with 4 and 6 g/kg FOS supplementation as compared to control.
Fructooligosaccharide;Digestive Enzyme;Intestinal Microflora;Intestinal Morphology;Pig;
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Asian-Australasian Journal of Animal Sciences, 2007. vol.20. 9, pp.1444-1452 crossref(new window)
Anonymous. 1999. How do mannanoligosaccharides work. Feeding Times (1):7-9.

Baily, J. S., L. C. Blankenship and N. A. Cox. 1991. Effect of fructooligosaccharide on Salmonella colonization of the chicken intestine. Poult. Sci. 70:2433-2438.

Bolduan, G., M. Beck and C. Schubert. 1993. Zur Wirkung von Oligosacchariden beim Ferkel. Arch. Anim. Nutr. 44:21-27. crossref(new window)

Bryant, M. P. and I. M. Allison. 1961. An improved non-selective culture medium for animal bacterial and its use in determining diurnal variation in numbers of bacteria in the rumen. J. Dairy Sci. 44:1446-1453.

Bryant, M. P. 1972. Commentary on the Hungate technique for culture for anaerobic bacteria. Am. J. Clin. Nutr. 25:1324-1330.

Bunce, T. J., M. D. Howard, G. L. Allee and L. W. Pace. 1995. Protective effects of fructooligosaccharide (FOS) in prevention of mortality and morbidity from infectious E.coli K: 88 challenge. J. Anim. Sci. 73(Suppl. 1):69(Abstr.).

Conway, P. L. 1994. The function of the gastrointestinal microflora and its regulation. In: Proceeding of the 6th international seminar on digestive physiology of pig. Sicuan: Sicuan science and technology press. pp. 233-242 (in Chinese).

Delzenne, N. M., N. Kok, M. F. Fiordaliso, D. M. Deboyser, F. M. Goethals and M. B. Roberfroid. 1993. Dietary fructooligosaccharides modify lipid metabolism in rats. Am. J. Clin. Nutr. 57(Suppl.):820S(Abstr.).

Erlanger, B. F., F. Edel and A. G. Cooper. 1966. The action of chymotrypsin on two new chromogenic substrates. Arch. Biochem. Biophys. 115:206–210. crossref(new window)

Erlanger, B. F., N. Kokowsky and W. Cohen. 1961. The preparation and properties of two new chromogenic substrates of trypsin. Arch. Biochem. Biophys. 95:271–278. crossref(new window)

Erlanson-Albertsson, C., A. Larsson and R. Duan. 1987. Secretion of pancreatic lipase and colipase from rat pancreas. Pancreas. 2:531-535. crossref(new window)

Farnworth, E. R., H. W. Molder, J. D. Jones, N. Cave, H. Yamazaki and A. V. Rao. 1992. Feeding Jerusalem artichoke flour rich in fructo-oligosaccharides to weanling pigs. Can. J. Anim. Sci. 72:977-980.

Fukuyasu, T., T. Oshida and K. Ashida. 1987. Effects of oligosaccharides on growth of piglets and on bacteria flora, putrefactive substances and volatile fatty acids in their feces. Bulletin of Anim. Hygience 26:15-22.

Gao, L. S. (ed.). 1998. Digestive physiology and health protection, 1th edn. Beijing: China curatorial science and technology press. pp. 173-230 (in Chinese).

Gibson, G. R. and X. Wang. 1994. Bifidogenic properties of different types of fructooligosaccharides. Food Microbiol. 11:491-498. crossref(new window)

Hidaka, H., T. Eida and T. Hamaya. 1986a. Livestock feed containing inulo- oligosaccharides and breeding of livestock by using the same. Eur. Pat. Appl. 017026A2:10.

Hidaka, H., T. Eida, T. Tokunaga and Y. Tashiro. 1986b. Effects of fructooligosaccharides on intestinal flora and human health. Bifidobact. Microflora 5:37-50.

Hidaka, H., Y. Tashiro and T. Eida. 1991. Proliferation of bifidobacteria by oligosaccharides and their useful effect on human health. Bifidobact. Microflora 10:65-79.

Holdeman, L. V., E. P. Cato and W. E. C. Moore (ed.). 1977. Anaerobic laboratory mannual, 4th edn. Blacksburg: Virginia Polytechnic Institute and State University.

Howard, M. D., D. T. Gordon, L. W. Pace, K. A. Garleb and M. S. Kerley. 1995. Effects of dietary supplementation with fructooligosaccharides on colonic microbiota populations and epithelial cell prolifertion in neonatal pigs. J. Gastroenterology Nutr. 21:297-303.

Ikegami, S., F. Tsuchihashi, H. Harada, N. Tsuchihashi, E. Nishidt and S. Innami. 1990. Effects of viscous indigestible polysaccharides on pancreatic-biliary secretion and digestive organs in rats. J. Nutr. 120:353-360.

Jensen, M. S., K. E. Bach Knudsen, J. Inborr and K. Jakobsen. 1998. Effect of $\beta$-glucanase supplementation on pancreatic enzyme activity and nutrient digestibility in piglets fed diets based on hulled and hulless barley varieties. Anim. Feed Sci. Technol., 72:329-345. crossref(new window)

Kim, M. and H. K. Shin. 1998. The water soluble extract of chicory influences serum and liver lipid concentrations, cecal short-chain fatty acid concentrations and fecal lipid excretion in rats. J. Nutr. 128:1731-1736.

Kornegay, E. T., C. M. Wood and L. A. Eng. 1992. Effectiveness and safety of fructooligosaccharides for pigs. Anim. Sci. Res. Report Virginia Agr. Exp. Station 10:19-20.

Lynn, K. R. and N. A. Clevette-Radford. 1984. Purification and characterization of hevin, a serin protease from Hevea brazilliensis. Biochemistry 23:963-964.

Mevissen-Verhage, E .A .E., J. H. Marcelis, N. M. de Vos and J. Verhoef. 1987. Bifidobacterium, Bacteroides and Clostridium spp. in faecal samples from breast-fed and bottle-fed infants with and without iron supplement. J. Clin. Microbiol. 25:285-289.

Munoa, F. J. and R. Pares. 1988. Selective medium for the isolation and enumeration of Bifidobacterium spp. Appl. Environ. Microbiol. 54:1715-1718.

National Research Council. 1998. Nutrient requirements of swine. I0th Ed. National Academy Press. Washington, DC.

Roberfroid, M. B., J. A. E. Vanloo and G. R. Gibson. 1998. The bifidogenic nature of chicory inulin and its hydrolysis products. J. Nutr. 128:11-19.

SAS. Institute Inc. 1989. SAS/STAT User's Guide, Version 6. SAS Institute Inc., Cary, North Carolina.

Sissons, J. W. 1989. Potential of probiotic organisms to prevent diarrhea and promote digestion in farm animals: a review. J. Sci. Food Agric. 49:1-13. crossref(new window)

Tokunaga, T., T. Oku and N. Hosoya. 1989. Utilization and excretion of a new sweetener, fructooligosaccharide in rats. J. Nutr. 119:553-559.

Xu, Z. R., C. H. Hu and M. Q. Wang. 2002. Effects of fructooligosaccharide on conversion of L-tryptophan to skatole and indole by mixed populations of pig fecal bacteria. J. Gen. Appl. Microbial. 48:83-89. crossref(new window)

Yason, C. V., B. A. Summers and K. A. Schat. 1987. Pathogenesis of rotavirus infection in various age groups of chickens and turkeys: pathology. Am. J. Vet. Res. 6:927-938

Zhan, C. 1998. The action of decreasing cholesterol of Bifidobacterium: precipitation and assimilation with biliary salt. Medicine in abroad: antibiotic. 19:146-149 (in Chinese).