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Characterization of Lactobacillus acidophilus Isolated from Piglets and Chicken
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 Title & Authors
Characterization of Lactobacillus acidophilus Isolated from Piglets and Chicken
Ahn, Y.T.; Lim, K.L.; Ryu, J.C.; Kang, D.K.; Ham, J.S.; Jang, Y.H.; Kim, H.U.;
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 Abstract
Lactic acid bacteria were isolated from piglets and chicken and characterized. Lactic acid bacteria showing resistance to low pH and bile, adhesion to intestinal epithelium cells, and the inhibition of Escherichia coli and Salmonella spp. were identified as Lactobacillus acidophilus. L. acidophilus PF01 survived for 2 h in MRS broth adjusted to pH 2. L. acidophilus CF07 was less resistant than L. acidophilus PF01 to pH 2, but survived at pH 2.5 for 2 h. Both of isolates were able to grow in MRS broth containing 0.3% (w/v) bile, with L. acidophilus CF07 being more tolerant to bile than L. acidophilus PF01. L. acidophilus PF01 and CF07 adhered specifically to the duodenal and jejunal epithelium cells of piglet, and the cecal and duodenal epithelium cells of chicken, respectively. Both of isolates did not adhere to the epithelium cells of the various animal intestines from which they were isolated. When L. acidophilus was cultured with E. coli and Salmonella spp. in MRS broth, MRS broth containing 2% skim milk powder or modified tryptic soy broth at , L. acidophilus PF01 and CF07 inhibited the growths of E. coli K88 and K99, and S. enteritidis and S. typhimurium, respectively. Both of isolates were found to possess the essential characteristics of probiotic lactic acid bacteria for piglet and chicken.
 Keywords
Adhesion;Acid tolerance;Bile tolerance;Lactic acid bacteria;Lactobacillus acidophilus;Probiotics;
 Language
English
 Cited by
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항생제 대체 생균제가 자돈의 생산성,영양소 이용률, 유해가스 발생량 및 분내 미생물 수에 미치는 영향,김종덕;정흥우;심금섭;박승용;주종철;송재준;이경호;박중국;박도연;김창현;

한국유기농업학회지, 2010. vol.18. 4, pp.527-539
2.
백합(Meretrix meretrix)식해에서 분리한 Pediococcus pentosaceus SH-10의 생균제적 특성,송현정;김강진;김희대;유정희;구재근;박권삼;

한국수산과학회지, 2011. vol.44. 6, pp.605-611 crossref(new window)
 References
1.
Ahn, Y. T., P. K. Shin and H. U. Kim. 1997. Growth Inhibition of Escherichia coli O157:H7 and Salmonella typhimurium by lactic acid bacteria and bifidobacteria. Kor. J. Food Hyg. Safety. 12:181-187.

2.
Ahn, Y. T., Y. H. Kim, E. J. Jung, J. H. Lim, H. J. Kang and H. U. Kim. 1999. Resistance of lactobacilli and bifidobacteria isolated from fermented milk products to low pH and bile acid. Kor. J. Anim. Sci. 41:335-342.

3.
Baranyi, J. and T. A. Roberts. (1994). A dynamic approach to predicting bacterial growth in food. Int. J. Food Microbiol. 23:277-294. crossref(new window)

4.
Barrow, P. A., B. E. Brooker, R. Fuller and M. J. Newport. 1980. The attachment of bacteria to the gastric epithelium of the pig and its importance in the microecology of the intestine. J. Appl. Bacteriol. 48:147-154.

5.
Conway, P. L., S. L. Gorbach and B. R. Goldin. 1987. Survival of lactic acid bacteria in the human stomach and adhesion to in the human stomach and adhesion to intestinal cells. J. Dairy Sci. 70:1-12.

6.
De Man, J. C., M. Rogosa and M. E. Sharpe. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23:130-135.

7.
Fuller, R. 1975. Nature of the determinant responsible for the adhesion of lactobacilli to chicken crop epithelial cells. J. Gen. Microbiol. 87:245-250.

8.
Fuller, R. 1978. Epithelial attachment and other factors controlling the colonization of the intestine of the gnotobiotic chicken by lactobacilli. J. Appl. Bacteriol. 45:389-395.

9.
Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66:365-378.

10.
Gilliland, S. E. 1979. Beneficial interrelationships between certain microorganisms and humans; candidate microorganisms for use as dietary adjuncts. J. Food Prot. 42:164-167.

11.
Gilliland, S. E. and M. L. Speck. 1977. Antagonistic action of Lactobacillus acidophilus toward intestinal and foodborne pathogens in associative cultures. J. Food Prot. 40:820-823.

12.
Gilliland, S. E., T. E. Staley and L. J. Bush. 1984. Importance of bile tolerance of Lactobacillus acidophilus used as dietary adjunct. J. Dairy Sci. 67:3045-3051.

13.
Gopal, A., N. P. Shah and H. Roginski. 1996. Bile tolerance, taurocholate deconjugation and cholesterol removal by Lactobacillus acidophilus and Bifidobacterium spp.. Milchwissenschaft. 51:619-623.

14.
Havenaar, R., B. T. Brink and J. H. J. Huis in'T Veld. 1992. Selection of strains for probiotic use. In: Probiotics (Ed. R. Fuller). CHAPMAN & HALL, London. pp.209-224.

15.
Hechard, Y., M. Dheibomez, Y. Cenatiempo and F. Letellier. 1990. Antagonism of lactic acid bacteria from goats' milk against pathogenic strains assessed by the 'sandwich method'. Lett. Appl. Microbiol. 11:185-188. crossref(new window)

16.
Jin, L. Z, Y. W. Ho, M. A. Ali, N. Abdullah and S. Jalaludin. 1996a. Effect of adherent Lactobacillus spp. on in vitro adherence of salmonellae to the intestinal epithelial cells of chicken. J. Appl. Bacteriol. 81:201-206.

17.
Jin, L. Z., Y. W. Ho, N. Abdullah, M. A. Ali and S. Jalaludin. 1996b. Antagonistic effects of intestinal Lactobacillus isolates on pathogens of chicken. Lett. Appl. Microbiol. 23:67-71. crossref(new window)

18.
Jin. L. Z., Y. W. Ho, N. Abdullah and S. Jalaludin. 1998. Acid and bile tolerance of Lactobacillus isolated from chicken intestine. Lett. Appl. Microbiol. 27:183-185. crossref(new window)

19.
Kandler, O. and N. Weiss. 1986. Genus Lactobacillus. In: Bergey's Manual of Systematic Bacteriology, Vol. 2 (Ed. P. H. A. Sneath, N. S. Mair, M. E. Sharpe and J. G. Holt). WILLIAMS & WILKINS, Baltimore. pp.1209-1234.

20.
Lee, Y. K. and S. Salminen. 1995. The coming of age of probiotics. Trends Food Sci. Technol. 6:241-245. crossref(new window)

21.
Mayra-Makinen, A., M. Manninen and H. Gyllenberg. 1983. The adherence of lactic acid bacteria to the columnar epithelial cells of pigs and calves. J. Appl. Bacteriol. 55:241-245.

22.
Ouwehand, A. C., P. V. Kirjavainen, C. Shortt and S. Salminen. 1999. Probiotics: mechanisms and established effects. Int. Dairy J. 9:43-52. crossref(new window)

23.
Pettersson, L., W. Graf, L. Alm, S. Lindwall and A. Stromberg. 1983. Survival of Lactobacillus acidophilus NCDO 1748 in the human gastrointestinal tract: I. Incubation with gastric juice in vitro. In: XV Symp. Swed. Nutr. Found, Stockholm. pp. 123-125.

24.
Reinheimer, J. A. M. R. Demkow and M. C. Candioti. 1990. Inhibition of coliform bacteria by lactic cultures. Aust. J. Dairy Technol. 45:5-9.

25.
Suegara, N., M. Morotomi, T. Watanabe, Y. Kawai and M. Mutai. 1975. Behaviour of microflora in the rat stomach: Adhesion of lactobacilli to the keratinized epithelial cells of the rat stomach in vitro. Infect. Immun. 12:173-179.

26.
Tannock, G. W. 1997. Probiotic properties of lactic acid bacteria: Plenty of scope for fundamental R & D. Trends Biotechnol. 15:270-274. crossref(new window)