Publisher : Asian Australasian Association of Animal Production Societies
DOI : 10.5713/ajas.2002.274
Title & Authors
Bioluminescent Determination of Lactose Secretion: A Measure of the In Vitro Performance of Mammary Acini from Lactating Rats Choi, B.H.; Stewart, K.W.; Davis, S.R.; Myung, K.H.;
A culture system for lactating rat mammary acini was evaluated, where the primary indicator of performance was lactose secretion, measured by a sensitive bioluminescence assay. Lactose secretion was reduced by half (p<0.01) over the first 6 h of culture by overnight feed withdrawal (FW) from tissue donors but was sensitive to increased glucose concentration in the culture media (p<0.001) up to 30 mM. Lactose production of cells from fed donors over the first 6 h in culture in 30 mM glucose was 8.9 fmol/cell/h - a rate calculated to be about half that in vivo. No significant difference was shown in lactose secretion by cells from fed or FW rats over 6-24 h. Lactose secretion was 3.6 fmol/cell/h by cells from fed animals in 40 mM glucose concentration media over the 6-24 h culture period. Addition of insulin to the culture media had no effect on rates of lactose secretion while addition of prolactin and hydrocortisone, with or without insulin, significantly (p<0.001) decreased lactose production over both 0-6 h and 6-24 h culture periods. Lactose synthesis in vitro was significantly enhanced by aeration of the media during collagenase digestion of mammary tissue (p<0.05). No improvement in lactose secretion was effected by shaking of cells during culture, Matrigel coating of culture dishes or change in cell density over a range up to 2.5 million cells per ml.
Nematicidal Activity of Verticillium saksenae A-1 against the Pine Wood Nematode Bursaphelenchus mucronatus,Oh, In-Jae;Ju, Wan-Taek;Kim, Young-Ju;Jung, Woo-Jin;Kim, Kil-Yong;Park, Ro-Dong;
한국키틴키토산학회지, 2014. vol.19. 2, pp.81-86
Arthur, P. G., J. C. Kent and P. E. Hartman. 1989. Microanalysis of the metabolic intermediates of lactose synthesis in human milk and plasma using bioluminescent methods. Anal. Biochem. 176:449-456.
Bussman, L. E., S. Ward and. N. J. Kuhn. 1984. Lactose and fatty acid synthesis in rat mammary gland. Biochem. J. 219:173-180.
Carrick, D. T. and N. J. Kuhn. 1978. Diurnal variation and response to food withdrawal of lactose synthesis in lactating rats. Biochem. J. 174:329-235.
Cline, P. R., P. O., Zammora, H. L. Hosick. 1982. Morphology and lactose synthesis in tissue culture of mammary alveoli isolated from lactating mice. In Vitro. 18:694-702.
Davis, S. R., S. J. Eichler, Callaghan, M. R. Callaghan, R. C. J. Grieve and C. G. Prosser. 1993. Factors affecting the production of lactose by ovine mammary cells in vitro. Proc. NZ Soc. Anim. Prod. 53:147-149.
Foster, R. C. 1978.Lactose synthesis and secretion by mammary epithelial cells in vitro, a comparative study. Comp. Biochem. Physiol. 61A:253-259.
Geursen, A, A. Carne and M. R. Grigor. 1987. Protein synthesis in mammary acini isolated from lactating rats: effect of maternal diet. J. Nutr. 117:769-775.
Geursen, A. and M. R. Grigor. 1987. Nutritional regulation of milk protein messenger RNA concentrations in mammary acini isolated from lactating rats. Biochem. Int. 15:873-879.
Grimble, R. F. and Y. K. C. Mansaray. 1987. Effects in rats of dietary protein inadequacy on lactose production, milk volume and components of the lactose synthesis complex. Ann. Nutr. Metab. 31:179-184.
Knight, C. H., A. H. Docherty and M. Peaker. 1984. Milk yield in rats in relation to the size of the mammary secretory cell population. J. Dairy Res. 51:29-35.
Linzell, J. L. 1972. Milk yield, energy loss in milk and mammary gland weight in different species. Dairy Science Abstracts 34:351-360.
Martin, R. J. and R. L. Baldwin. 1971. Effects of insulin on isolated rat mammary cell metabolism glucose utilisation and metabolite patterns. Endocrinology 89:1263-1269.
Rao, D. R, H. M. Hegarty and B. L. Larson. 1975. Effects of cell density on lactose synthesis in bovine mammary cell cultures. J. Dairy Sci. 58:159-163.
SAS Institute Inc. 1997. SAS Users Guide: Release 6.07. Cary, North Carolina, SAS Institute.
Wheeler, T. T., M. R. Callaghan, S. R. Davis, C. G. Prosser and R. J. Wilkins. 1995. Milk protein synthesis, gene expression and hormonal responsiveness in primary culture of mammary cells from lactating sheep. Exp. Cell. Res. 217:346-351.
Wilde, C. J. and N. J. Kuhn. 1979. Lactose synthesis in the rat, and the effects of litter size and malnutrition. Biochem. J. 182:287-294.