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Secretory Proteins from Goat Oocytes Matured in Culture
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 Title & Authors
Secretory Proteins from Goat Oocytes Matured in Culture
Malakar, Dhruba; Majumdar, A.C.;
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In this experiment, oocytes were collected from goat ovaries available in slaughterhouse by follicle puncture method. Morphologically culturable type of oocytes which having compact, multilayered cumulus granulosa cell complex and evenly granulated cytoplasm, was separated under a stereozoom microscope. Oocytes were washed thoroughly in maturation medium containing TCM-199, estradiol-, 0.5 FSH, LH, 3 mg/ml BSA and 10% estrus goat serum. Washed oocytes were cultured into maturation medium on granulosa cell monolayer. Culture plate was then kept into incubator at , maximum humidity and 5% for 18 h. After maturation the oocytes were washed thoroughly with maturation medium containing polyvinyl alcohol (PVA) without serum and BSA and further cultured for 12 h for secretory proteins of oocytes. PVA medium was collected, pooled and concentrated by 5000 cut off centrisart. Secretory proteins were separated on 12.5% SDS-PAGE. A total number of 3.41 oocytes per ovary were obtained and 2.17 culturable oocytes per ovary were cultured into maturation medium. After 18 h of maturation, 4,567 oocytes (1.82 oocytes per ovary) were further cultured into serum and BSA free PVA medium for its secretory proteins. Four secretory proteins of oocytes with approximately molecular weight of 45, 55, 65 and 95 kDa were obtained on SDS-PAGE in silver staining and three proteins with approximately molecular weight of 45, 55 and 65 kDa in Coomassie brilliant blue staining. In conclusion, four secretory proteins with approximately molecular weight of 45, 55, 65 and 95 kDa was obtained from in vitro cultured oocytes of goats.
Goat Oocyte;Maturation;Granulosa Cell Monolayer;Polyvinyl Alcohol;Oocyte Secretory Proteins;IVM;
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Bagger, P. W., A. G. Byskov, M. D. Christiansen, L. Bang and L. Mortensen. 1993. Lithium stimulates the first meiotic division in mouse oocytes. Acta. Obstet. Gynaecol. Scand. 72:514-519. crossref(new window)

Bonde, S. W. 1995. Fertilizing and cleaving ability of follicular oocytes in domestic goat matured in vitro. Ph.D. Thesis, submitted to Deemed university, Indian Veterinary Research Institute, Izatnagar, (U.P.) India.

Carnoll, J., D. G. Whittingham and M. J. Wood. 1991. Effect of gonadotropin environment on growth and development of isolated mouse oocytes from frozen primary follicles. J. Reprod. Fertil. 93:71-79. crossref(new window)

Cognie, Y., N. Crozet, Y. Guerin, N. Poulin, J. Bezard, G. Duchamp, M. Magistrini and E. Palmer. 1992. In vitro fertilization in sheep, goats and horses. Ann. De. Zootech. 41: 353-359. crossref(new window)

Crozet, M., M. Ahmed-Ali and M. P. Dubos. 1995. Developmental competence of goat oocytes from follicles of different size categories following maturation, fertilization and culture in vitro. J. Reprod. Fertil. 103:293-298. crossref(new window)

Eppig, J. J., R. M. Schultz, M. O'Brien and P. Chesnel. 1994. Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev. Biol. 164:1-9. crossref(new window)

Gall, L., F. Le Gal and V. De Smend. 1993. Protein phosphorylation patterns during in vitro maturation of the goat oocyte. Mol. Reprod. Dev. 36:500-506. crossref(new window)

Hue, I., T. Dedieu, D. Huneau, S. Ruffini, L. Gall and N. Crozet. 1997. Cyclin B1 expression in meiotically competent and incompetent goat oocytes. Mol. Reprod. Dev. 47:222-228. crossref(new window)

Hunter, A. G. and P. M. Moor. 1987. State-dependent effects of inhibiting ribonucleic acids and protein synthesis on meiotic maturation of bovine oocytes in vitro. J. Dairy Sci. 70:1646-1651.

Kastrop, P. M. M., M. M. Bevers, O. H. J. Destree and T. A. M. Kruip. 1990b. Changes in protein synthesis and phosphorylation patterns during bovine oocyte maturation in vitro. J. Reprod. Fertil. 90:305-310. crossref(new window)

Keskintepe, L. and B. G. Brackett. 1996. In vitro developmental competence of in vitro matured bovine oocytes fertilized and cultured in completely defined media. Biol. Reprod. 55:333-339. crossref(new window)

Keskintepe, L., C. A. Burnley and B. G. Brackett. 1995. Production of viable bovine blastocysts in defined in vitro condition. Biol. Reprod. 52:1410-1417. crossref(new window)

King, W. A., D. Bousquet, T. Greve and A. K. Goff. 1986. Meiosis in bovine oocytes matured in vitro and in vivo. Acta. Vet. Scand. 27:267-279.

Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685. crossref(new window)

Liu, L., Y. Dai and R. M. Moor. 1997. Role of secreted proteins and gonadotrophins in promoting full maturation of porcine oocytes in vitro. Mol. Reprod. Dev. 47:191-199. crossref(new window)

Liu, Z., R. H. Foote and X. Yang. 1995. Development of early bovine embryos in co-culture with KSOM and taurine, superoxide dismutase or insulin. Theriogenology 44:741-750. crossref(new window)

Meng, L., J. Rutledge, Y. Zhu, G. M. Kidder, F. Khamsi and D. T. Armstrong. 1996. Role of germinal vesicle on protein synthesis in rat during in vitro maturation. Mol. Reprod. Dev. 43:228-235. crossref(new window)

Naqvi, S. M. K., M. L. Madan, M. S. Chauhan, S. K. Singla and R. S. Manik. 1992. In vitro fertilization of in vitro matured goat oocytes and development of embryos in oviductal epithelial cell co-culture medium. In: Proc. of V Int. Conf. on goat, March 2-8, 1992, New Delhi, Vol. I p. 358(Abstr.).

Okijama, T., T. Tanabe and T. Yasuda. 1993. Non urea sodium dodecyl sulphate polyacrylamide gel electrophoresis with high molarity buffer for separation of proteins and peptides. Anal. Biochem. 211:293-300. crossref(new window)

Park, E. H., R. C. Chain, H. M. Chung, J. G. Lim, J. J. Ko and K. Y. Cha. 1999. The stage of embryonic genome activation in bovine embryos following in vitro fertilization. Theriogenology 51:180-196. crossref(new window)

Rabahi, F., D. Monniaux, C. Pisselet, D. Chupin and P. Durand. 1991. Qualitative and quantitative changes in protein synthesis of bovine follicular cells during the preovulatory period. Mol. Reprod. Dev. 30:265-275. crossref(new window)

Rehman, N. U., M. Saarwar and H. A. Samad. 2001. In vitro production of bovine embryos-A review. Asian-Aust. J. Anim. Sci. 14(9):1342-1351.

Sambrook, J., E. F. Fritoh and T. Maniatis. 1989. Molecular cloning-A laboratory mannual. 2nd edn. New York, Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

Satoh, T., K. Kobayashi, S. Yamashita, M. Kikuchi, Y. Sendai and H. Hoshi. 1994. Tissue inhibitor of metalloproteinases (TIMP-1) produced by granulosa and oviduct cells enhances in vitro development of bovine embryos. Biol. Reprod. 50:835-844. crossref(new window)

Sharma, G. T., A. C. Majumdar and S. W. Bonde. 1996. Chronology of maturational events in goat oocytes cultured in vitro. Small Ruminant Research. 22:25-30. crossref(new window)

Sharma G. T., A. Teotia and A. C. Majumdar. 2001. Meiotic competence of caprine oocytes during IVM on granulosa cell monolayers developed from small and large follicles in comparison to the granulosa cell co-culture. Asian-Aust. J. Anim. Sci. 14(6):777-784.

Tatemoto, H. and T. Terada. 1995. On the C-mos proto-oncogene product during meiotic maturation in bovine oocytes cultured in vitro. J. Exp. Zool. 272:159-162. crossref(new window)

Teotia, A., 1998. Fertilization and developmental competence of caprine oocytes matured over granulosa cell monolayer. M.V.Sc. Thesis, Deemed University, Indian Veterinary Research Institute, Izatnagar, U.P., India.

Tyagi, S., G. T. Sharma and A. C. Majumdar. 1997. Meiotic competence of goat oocytes matured on granulosa cell monolayers. Theriogenology 47:203(Abstr.). crossref(new window)

Wu, B., G. G. Ignotz, W. B. Currie and X. Yang. 1996. Temporal distinctions in the synthesis and accumulation of proteins by oocytes and cumulus cells during maturation in vitro of bovine oocytes. Mol. Reprod. Dev. 45:560-565. crossref(new window)

Xia, G., A. G. Byskov and C. Y. Andersen. 1994. Cumulus cells secrete a meiosis-inducing substance by stimulation with forskolin and dibutyric cyclic adenosine monophosphate. Mol. Reprod. Dev. 39:17-24. crossref(new window)

Younis, A. I., K. A. Zuelke, K. M. Harper, M. A. L. Oliveira and B. G. Brackett. 1991. In vitro fertilization of goat oocytes. Biol. Reprod. 44:1177-1182. crossref(new window)