JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effect of Aflatoxin B1 on the Function of Peritoneal Macrophage from Mule Duck
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Aflatoxin B1 on the Function of Peritoneal Macrophage from Mule Duck
Cheng, Yeong-Hsiang; Shen, Tian-Fuh; Pang, Victor Fei; Chen, Bao-Ji;
  PDF(new window)
 Abstract
This study was conducted to investigate the effect of aflatoxin () alone or mixed function oxidase (MFO)-activated on various functions of mule duck peritoneal macrophages. Duck peritoneal macrophages were incubated with 0, 5, 10, 20, 50 and for 12 h. The cell viability significantly declined as the concentration of increased and more obviously detrimental effects was noticed in MFO-metabolized treatments. Either in opsonized or unopsonized Candida albicans, phagocytotic ability of macrophages was decreased with the elevation of the concentration of . Significantly higher levels of macrophages were damaged in MFO-metabolized than alone in concentrations above . The cytotoxicity activity was in the range of 41 to 33% after exposure to 5 to , and a significant higher TNF-like substance secretion by lipopolysaccharide (LPS) stimulation was obtained. When LPS was present in the medium, the percentage of cytotoxicity was higher than all treatments of both with and without MFO-activation in the absence of LPS. The results suggest that MFO-metabolized can alter cell viability and morphology of duck macrophages more than administered alone. Both with and without MFOactivation, has detrimental effects on phagocytotic ability and TNF-like substance secretion, increasing with level of .
 Keywords
Aflatoxin ;Macrophage;Immune Function;Mule Duck;
 Language
English
 Cited by
1.
Induction of Changes in Morphology, Reactive Nitrogen/Oxygen Intermediates and Apoptosis of Duck Macrophages by Aflatoxin B1,;;;

아세아태평양축산학회지, 2002. vol.15. 11, pp.1639-1645 crossref(new window)
 References
1.
Aderem, A. and D. M. Underhill. 1999. Mechanism of phagocytosis in macrophages. Annu. Rev. Immunol. 17:593-623. crossref(new window)

2.
Blount, W. P. 1961. Turkey X disease. J. Br. Turkey Federation. 9:52.

3.
Chang, C. F. and P. B. Hamilton. 1979. Impaired phagocytosis by heterophils from chickens during aflatoxicosis. Toxicol. Appl. Pharmacol. 48:459-466. crossref(new window)

4.
Cheng, Y. S. and V. F. Pang. 1995. Effect of aflatoxin on lymphocyte transformation in duck. J. Agric. Assoc. China 170:147-155.

5.
Cook, J. C. and E. Hodgson. 1983. Induction of cytochrome P450 by methylenedioxyphenyl compounds:Importance of the methylene carbon. Toxicol. Appl. Pharmacol. 131-139. crossref(new window)

6.
Hamilton, T. A. and D. O. Adams. 1987. Molecular mechanisms of signal transduction in macrophages. Immunol. Today 8:151-158. crossref(new window)

7.
Kadian, S. K., D. P. Monga and M. C. Goel. 1988. Effect of aflatoxin $B_1$ on the delayed type hypersensitivity and phagocytic activity of reticuloendothelial system in chickens. Mycopathologia 104:33-36. crossref(new window)

8.
Karenlampi, S. O. 1987. Mechanism of cytotoxicity of aflatoxin $B_1$: Role of cytochrome P450. Biochem. Biophys. Res. Commun. 145:854-860. crossref(new window)

9.
Kimball, J. W. 1990. Introduction to Immunology. Macmillan Publishing Co., New York; NY. USA. pp. 232-345.

10.
Lorr, N. A., K. A. Golemboski, R. R. Dietert and S. E. Bioom. 1990. Cytochrome P450 catalyzed activity in the bursa, thymus, peritoneal exudate cell (PECs), and liver of the 4 weeks old chicken after incubation with 3, 4, 3', 4' tetrachlorobiphenyl (TCB). Poult. Sci. 69:85(Abstr.).

11.
Lowry, O. H., N. J. Rosenbrough, A. L. Farr and R. L. Randall. 1951. Protein measurement with folin phenol reagent. J. Biol. Chem. 193:265-275.

12.
Michael, G. Y., P. Thaxton and P. B. Hamilton. 1973. Impairment of reticuloendothelial system of chickens during aflatoxicosis. Poult. Sci. 52:1206-1207.

13.
Moon, E. Y., D. K. Rhee and S. Pyo. 1999. In vitro suppressive effect of aflatoxin $B_1$ on murine peritoneal macrophages functions. Toxicol. 171-179.

14.
Neldon-Ortiz, D. L. and M. A. Qureshi. 1991. Direct and microsomal activated aflatoxin $B_1$ exposure and its effects on turkey peritoneal macorphage functions in vitro. Toxicol. Appl. Pharmacol. 109:432-442. crossref(new window)

15.
Neldon-Ortiz, D. L. and M. A. Qureshi. 1992. The effects of direct and microsomal activated aflatoxin $B_1$ on chicken peritoneal macrophages in vitro. Vet. Immunol. Immunopathol. 31:61-67. crossref(new window)

16.
Pang, V. F. 1994. The immunity effect of aflatoxin in pigs. Life Sci. Newsletter. 8:1-9.

17.
Pang, V. F., J. S. Chao, M. T. Chiou, S. S. Hsiao and C. R. Jeng. 1997. The development of a bioassay for tumor necrosis factor derived from porcine alveolar macrophages using MTT colorimetric measurement in a cell line, PK-15. J. Chin. Soc. Vet. Sci. 23:320-331.

18.
Qureshi, M. A., R. R. Dietert and L. D. Bacon. 1986. Genetic variation in the recruitment and activation of chicken peritoneal macrophage. Proc. Soc. Exp. Biol. Med. 181:560-566.

19.
Qureshi, M. A. and L. Miller. 1991. Signal requirements for the acquisition of tumoricidal competence by chicken peritoneal macrophages. Poult. Sci. 70:530-538.

20.
Qureshi, M. A. and W. M. Hagler Jr. 1992. Effect of fumonisin-$B_1$ exposure on chicken macrophage function in vitro. Poult. Sci. 71:104-112.

21.
Richard, J. L. and J. R. Thurston. 1975. Effect of aflatoxin on phagocytosis of Aspergillus fumigatus spores by rabbit alveolar macrophages. Appl. Microbiol. 30:40-47.

22.
SAS Institute Inc. 1989. SAS/STAT Guide for personal computers. Release 6.03th Edition. SAS Institute Inc. Cary, NC, USA.

23.
Shen, T. F., C. C. Yen and C. C. Kuo. 1988. The effect of low levels of aflatoxin in the diet on the performance of mule ducks. J. Agric. Assoc. China 143:68-77.

24.
Skamene, E. and P. Gros. 1983. Role of macrophages in resistance against infectious diseases. Clinic. Immunol. Allergy 3:539-560.

25.
Smith, J. E. and M. O. Moss. 1985. Mycotoxins: Formation, Analysis, and Significance. John Wiley and Sons Publishing. New York, USA. pp. 12-15.

26.
Swenson, O. H. and E. C. Miller and J. A. Miller. 1974. Aflatoxin $B_1$-2,3-oxide: Evidence for its formation in rat liver in vivo and by human liver microsomes in vitro. Biochem. Biophys. Res. Commun. 60:1036-1043. crossref(new window)

27.
Takai, N., M. Kataoka, Y. Higochi, K. Matsuura and S. Yamamotok. 1997. Primary structure of rat CD14 and characteristics of rat CD14, cytokine and NO synthetase mRNA expression in mononuclear phagocyte system cells in response to LPS. J. Leukocyte Biol. 61:736-744.

28.
Trembicki, K. A., M. A. Qureshi and R. R. Dietert. 1984. Avian peritoneal exudate cells: a comparison of stimulation protocols. Dev. Comp. Immunol. 8:395-402. crossref(new window)

29.
Wogan, G. N. and P. M. Newberne. 1966. Dose characteristics of aflatoxin $B_1$ carcinogenesis in the rat. Cancer Res. 57:403-407.

30.
Wright, S. D., R. A. Ramos, P. S. Tobias, R. J. Ulevitch and J. C. Mathison. 1990. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249:1431-1433. crossref(new window)