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Effects of Active Immunization against Somatostatin or its Analogues on Milk Protein Synthesis of Rat Mammary Gland Cells
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Effects of Active Immunization against Somatostatin or its Analogues on Milk Protein Synthesis of Rat Mammary Gland Cells
Kim, J.Y.; Cho, K.K.; Chung, M.I.; Kim, J.D.; Woo, J.H.; Yun, C.H.; Choi, Y.J.;
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Effects of active immunization against native 14-mer somatostatin (SRIF, somatotropin releasing inhibiting factor) and its two 14-mer-somatostatin analogues on the milk production in rat mammary cells were studied. Native SRIF, Tyr11-somatostatin (Tyr11-SRIF), and D-Trp8, D-Cys14-somatostatin (Trp8Cys14-SRIF) were conjugated to bovine serum albumin (BSA) for immunogen preparation. Twenty-four female Sprague-Dawley rats were divided into four groups and immunized against saline (Control), SRIF, Tyr11-SRIF, and Trp8Cys14-SRIF at five weeks of age. Booster immunizations were performed at 7, 9, and 11 weeks of age. SRIFimmunized rats were mated at 10 weeks of age. The blood and mammary glands were collected at day 15 post-pregnancy and -lactation. To measure the amount of milk protein synthesis in the mammary gland, mammary cells isolated from the pregnant and the lactating rats, were cultured in the presence of -lysine. No significant differences in growth performance, concentration of growth hormone in the circulation, and the amount of milk protein synthesis were observed among the groups. Inductive levels of serum anti-SRIF antibody in the SRIF and Tyr11-SRIF groups but not in the Trp8Cys14-SRIF group, were significantly higher than that of the control group during the pregnancy and lactation periods. The result suggests that active immunization against native 14-mer SRIF and Tyr11-SRIF was able to induce anti-SRIF antibodies, but did not affect the milk protein synthesis.
Somatostatin;Active Immunization;GH Concentration;Milk Protein Synthesis;
 Cited by
Arimura, A, W. D. Smith and A. V. Schally. 1976. Blockage of the stress-induced decrease in blood GH by anti-somatostatin serum in rats. Endocrinology 98:540-543. crossref(new window)

Baumon, D. E., P. J. Epard and M. J. De Geeter. 1985. Response of high-producing dairy cows to long-term treatment with pituitary somatotropin and recombinant somatotropin. J. Dairy Sci. 68:1352-1362. crossref(new window)

Bchini, O., A. C. Andres, B. Schubaur, M. Methtali, M. Lemeur, R. Lathe and P. Gerlinger. 1991. Precocious mammary gland development and milk protein synthesis in transgenic mice ubquitously expressive human growth hormone. Endocrinology 128:539-546. crossref(new window)

Chihara, K., N. Minamitane, H. Kaji, H. Kodama, T. Kita and T. Fujita. 1983. Human pancreatic growth hormone-releasing factor stimulates release of growth hormone in conscious unrestrained male rabbits. Endocrinology 113:2081-2085. crossref(new window)

Coligan, J. E., A. M. Kruisbeek, D. H. Margulies, E. M. Shevach and W. Trober. 1991. In: Current Protocols in Immunolgy (Ed. J. E. Coligan). John Wiley & Sons, USA, Vol. 1, pp. 9.4.1-9.4.3.

Dawson, J. M., J. B. Soar, P. J. Buttery, J. Craigon, M. Gill and D. Beever. 1997. The effect of immunization against somatostatin and $\beta$-agonist administration alone and in combination on growth and carcass composition in young steers. Anim. Sci. 64:37-51. crossref(new window)

Deligeorgis, S. G., E. Rogdakis, A. Mantzios, E. Nikolaou, G. Evangelatos and V. Sotitriadis. 1988. A note on the effect of active immunization against somatostatin on milk production and growth in sheep. Anim. Prod. 46:304-308. crossref(new window)

Desai D. C., T. M. O'Dorisio, W. J. Schirmer, S. S. Jung, H. Khabiri, V. Villanueva and E. W. Martin. 2001. Serum pancreastatin levels predict response to hepatic artery chemoembolization and somatostatin analogue therapy in metastatic neuroendocrine tumors. Regul Pept. 96:113-117. crossref(new window)

Du, Z. L. and R. R. Hacker. 1992. An investigation of immunization against somatostatin by measuring growth and carcass parameters in gilts, Asian-Aus. J. Anim. Sci. 5:665-671.

Du, Z. L. and R. R Hacker. 1993. An investigation of immunization against somatostatin by measuring antibody titres, somatostatin and somatotropin profiles in gilts. Asian-Aus. J. Anim. Sci. 6:211-218.

Dubreuil, P., G. Pelletier, D. Petitclerc, H. Lapierre, P. Gaudreau and P. Brazeau. 1989. Effects of active immunization against somatostatin on serum growth hormone concentration in growing pigs: Influence of fasting and repetitive somatocrinin injections. Endocrinology 125:1378-1384. crossref(new window)

Etherton, T. D., J. P. Wiggins, C. S. Chunt, C. M. Evock, J. F. Rebhun and P. E. Walton. 1986. Stimulation of pig growth performance by porcine growth hormone and growth hormone-releasing factor. J. Anim. Sci. 6:1389-1399.

Farmer, C., P. Dubreuil, G. Pelletier, D. Petitclerc and P. Brazeau. 1990. Active immunization against somatostatin in gestating gilts and the effect of transferred immunity on piglets. Canadian J. Anim. Sci. 70:211- 218. crossref(new window)

Farmer, C. and P. Brazeau. 1992. Colostum and milk composition of sows immunized against somatostatin or its carrier protein. Canadian J. Anim. Sci. 72:177-180. crossref(new window)

Flint, D. J., E. Tonner, J. Beattie and D. Panton. 1992. Investigation of the mechanism of action of growth hormone in stimulating lactation in the rat. J. Endocrinology 134:377-383. crossref(new window)

Flint, D. J. and M. Gardner. 1994. Evidence that growth hormone stimulates milk synthesis by direct action on the mammary gland and that prolactin exerts effects on milk secretion by maintenance of mammary deoxyribonucleic acid content and tight junction status. Endocrinology 135:1119-1124. crossref(new window)

Frenyo, V. L., G. Pethes, T. Antal and I. Szabo. 1981. Changes in colostral and serum IgG content in swine in relation to time. Veterinary Reseach Communication 4:275-282. crossref(new window)

Garssen, G. J., A. M. Welling and G. S. D. Spencer. 1987. Active immunization against somatostatin increases milk yield in goats. Netherland J. Agric. Sci. 35:84-86.

Kim, J. H., M. I. Chung, K. K. Cho, C. D. Kim, Y. S. Kim, J. H. Woo and Y. J. Choi. 2001. Effects of active immunization against somatostatin or somatostatin analogues on male rat growth. 2001. Food and Agricultural Immunology 13:141-149. crossref(new window)

Laarveld, B., R. K. Chaplin and D. E. Kerr. 1986. Somatostatin immunization and growth of lambs. Canadian J. Anim. Sci. 66:77-83. crossref(new window)

Lanzi, R. and G. S. Tannenbaum. 1992. Time course and mechanism of growth hormone' negative feedback effect on its own spontaneous release. Endocrinology 130:780-788. crossref(new window)

Liberti, J. P. and G. S. Joshi. 1986. Synthesis and secretion of phosphorylated growth hormone by rat pituitary gland in vitro. Biochemistry Biophys Research Communication 137:806-812. crossref(new window)

Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Bio. Chem. 193:265-275.

Machen, R. V., G. T. Schelling, F. M. Buyers and L. W. Greene. 1987. Response to active immunization against somatostatin in finishing steers. J. Anim. Sci. 66(Suppl. 1):246(Abstr.).

McMahon, C. D., R. P. Radcliff, K. J. Lookingland and H. A. Tucker. 2001. Neuroregulation of growth hormone secretion in domestic animals. Domest Anim Endocrinol. 20:65-87. crossref(new window)

Magnan, E., L. Mazzocchi, M. Cataldi, V. Guillaume, A. Dutour, F. Dadoun, Y. Le Bouc, N. Sauze, M. Renard, B. Conte-Devolx and C. Oliver. 1995. Effect of actively immunizing sheep against growth hormone-releasing hormone or somatostatin on spontaneous pulsatile and neostigmineinduced growth hormone secretion. J. Endocrinol. 144:83-90. crossref(new window)

Mears, G. J. 1990. Immunization of lambs against somatostatin to improve growth rate. Canadian J. Anim. Sci. 70:1091-1097. crossref(new window)

Mears, G. J. 1995. The relationship of plasma somatosmedin (IGF-1) to lamb growth rate. Canadian J. Anim. Sci. 75:327-331. crossref(new window)

Myung, K. H. 1990. Effects of recombinant bovine somatotropin on milk production and milk composition in dairy cows. Asian-Aus. J. Anim. Sci. 3247-252.

Painson, J. and D. S. Tannenbaum. 1991. Sexual dimorphism of somatostatin and growth hormone-releasing factor signaling in the control of pulsatile growth hormone secretion in the rat. Endocrinology 128:2858-2866. crossref(new window)

Petitclerc, G., P. Dubreuil, H. Lapierre, C. Farmer and P. Brazeau. 1988. Effects of active immunization against somatostatin and growth hormone-releasing factor infusion and growth secretion in dairy heifers. J. Anim. Sci. 66(Suppl. 1):389(Abstr.).

SAS. 1991. SAS. STAT User's guide version 6, fourth edtion. SAS Institute Inc., Cary, NC, USA.

Shulkes, A., C. Moore, S. Kolivas and J. Whitley. 1999. Active immunoneutralization of somatostatin in the sheep: effects on gastrointestinal somatostatin expression, storage and secretion. Regul Pept. 82:59-64. crossref(new window)

Sinha, Y. N. and B. P. Jacobsen. 1988. Three growth hormone- and two prolactin-related novel peptides of Mr 13,000-18,000 identified in the anterior pituitary. Biochemistry Biophys Research Communication 156:171-179. crossref(new window)

Spencer, G. S. G., G. J. Garssen and I. E. Hart. 1983. A novel approach to growth promotion using auto-immunization against somatostatin. I. Effects on growth and hormone levels in lambs. Livest. Prod. Sci. 10:25-37. crossref(new window)

Stolar, M. W. and G. Baumann. 1986. Big growth hormone forms in human plasma: immunochemical evidence for their pituitary origin. Metabolism 35:75-77. crossref(new window)

Tannenbaum, G. S., J. Epelbaum, E. Cole, P. Brazeau and J. B. Martin. 1978. Anti-serum to somatostatin reverses starvationinduced inhibition of hormone but not insulin secretion. Endocrinology 102:1909-1914. crossref(new window)

Tannenbaum, G. S. and N. Ling. 1984. The interrelationship of growth hormone-releasing factor and somatostatin in generation of the ultradian rhythm of growth hormone secretion. Endocrinology 115:1952-1959. crossref(new window)

Tannenbaum G. S. and C. Y. Bowers. 2001. Interactions of growth hormone secretagogues and growth hormone-releasing hormone/somatostatin. Endocrine. 14:21-7. crossref(new window)

Tannenbaum, G. S., W. Gurd, M. Lapointe and M. Pollak. 1992. Tamoxifen attenuates pulsatile growth hormone secretion:Mediation in part by somatostatin. Endocrinology 130:3395-4001. crossref(new window)

Travers, M. T., M. C. Barber, E. Tonner, L. Quarrie, C. J. Wilde and D. J. Flint. 1996. The role of prolactin and growth hormone in the regulation of casein gene expression and mammary cell survival: Relationships to milk synthesis and secretion. Endocrinology 137:1530-1539. crossref(new window)

Wehrenberg, W. B., P. Brazeau, R. Luben, P. Bohlen and R. Guillemin. 1982. Inhibition of the pulsatile secretion of growth hormone by monoclonal antibodies to the hypothalamic growth releasing factor (GRF). Endocrinology 111:2147-2153. crossref(new window)

Wehrenberg, W. B., P. Brazeau, R. Luben, N. Ling and R. Guillemin. 1983. A noninvasive functional lesion of the hypothalamo-pituitaty axis for the study of growth hormonereleasing factor. Neuroendocrinology 36:489-493. crossref(new window)

Yi, I. G., D. S. Lim, J. Y. Kim, J. M. Chung, Y. J. Choi and Y. S. Kim. 1999. Effects of active immunization against somatostatin or somatostatin analogues on millk production in rats. Nutrition Research 19:1061-1072. crossref(new window)

Yoyoka, S. and H. G. Friesen. 1986. Human growth hormone (GH)-releasing factor stimulates and somatostatin inhibits the release of rat GH variants. Endocrinology 119:2097-2105. crossref(new window)