Advanced SearchSearch Tips
Age-related Changes in Plasma Leptin from Early Growing to Late Finishing Stages of Castrated Holstein Steers: Utilizing Multi-species Leptin RIA
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Age-related Changes in Plasma Leptin from Early Growing to Late Finishing Stages of Castrated Holstein Steers: Utilizing Multi-species Leptin RIA
Vega, R.A.; Lee, H.G.; Kuwayama, H.; Matsunaga, N.; Hidari, H.;
  PDF(new window)
This experiment was performed to understand the changes in plasma leptin in association with plasma IGF-1, body weight and ADG from early growing to late finishing stages of Holstein steers. Blood collection was performed by arterial vein puncture at selected monthly ages of 1 (54 kg), 2.6 (103 kg), 7.2 (205 kg), 13.5 (314 kg), 16.9 (414 kg), 22.2 (550 kg), 24.9 (626 kg) and 27.4 months (695 kg). The blood was analyzed for leptin using the multi-species leptin RIA with recombinant bovine leptin (rbleptin) as standard, plasma IGF-1 was also measured using RIA. Against the standard rbleptin, the multi-species Leptin RIA system's sensitivity, cross reactivity, slope and recovery of 41.0 ng/ml rbleptin in plasma were 4.9 ng/ml, 11.22%, -1.396 and 97.8%, respectively. Plasma leptin measured were more than 5.0 ng/ml, which enable multi-species RIA system to investigate plasma leptin in normal growing steers. Body weight resulted to a highly significant second-degree polynomial relationship with plasma leptin (q=0.54, p<0.0001) and plasma IGF-1 (q=0.44, p<0.0001) from 1 to 27.4 monthly ages. However, the second-degree polynomial curve of plasma leptin and IGF-1 differs showing a concave and convex curvilinear relationship, respectively. ADG was not significantly associated to plasma leptin (r=0.06, p>0.05) and plasma IGF=1 (r=0.06, p>0.05) from 1 to 27.4 monthly ages. Low coefficient, but significant associated increase of plasma leptin and IGF-1 (r=0.12, p<0.008) from 1 to 27.4 months was observed. The uncoordinated increases of plasma IGF-1 at growing and plasma leptin at fattening period, may indicate (1) indirect involvement of endogenous IGF-1 on leptin secretion, and (2) IGF-1 level may signify lean and bone accretion while plasma leptin may mirror body fatness across the monthly ages of Holstein steers.
Multi-species Leptin RIA;Holstein Steer;Leptin;IGF-1;Growth Stages;
 Cited by
Development of Bovine Specific Leptin Radioimmunoassay and Relationship of Plasma Leptin with Vitamin A and Age of Wagyu,;;;;;;;;;;;;;

아세아태평양축산학회지, 2008. vol.21. 9, pp.1286-1295 crossref(new window)
Physiological Roles of Adipokines, Hepatokines, and Myokines in Ruminants,;;;;;

아세아태평양축산학회지, 2016. vol.29. 1, pp.1-15 crossref(new window)
Influence of the diet and grazing on adipose tissue lipogenic activities and plasma leptin in steers, animal, 2007, 1, 09  crossref(new windwow)
- Invited Review - Physiological Roles of Adipokines, Hepatokines, and Myokines in Ruminants, Asian-Australasian Journal of Animal Sciences, 2015, 29, 1, 1  crossref(new windwow)
Agriculture, Forestry and Fisheries Research Council Secretariat. 1995. Japanese Feeding Standard for Beef Cattle. Agriculture Forestry and Fisheries Research Council Secretariat. Tokyo. 1995.

Arslanian, S., C. Suprasongsin, S. C. Kalhan, A. L. Drash, R. Brna and J. E. Janosky. 1998. Plasma leptin in children: relationship to puberty, gender, body composition, insulin sensitivity and energy expenditure. Metabolism 47(3):309-312. crossref(new window)

Barb, C. R., X. Yan, M. J. Azain, R. R. Kraeling, G. B. Rampacek and T. G. Ramsay. 1998. Recombinant porcine leptin reduces feed intake and stimulates growth hormone secretion in swine. Domest. Anim. Endocrinol. 15(1):77-86. crossref(new window)

Blache, D., R. L. Tellam, L. M. Chagas, M. A. Blackberry, P. E. Vercoe and G. B. Martin. 2000. Level of nutrition affects leptin concentrations in plasma and cerebrospinal fluid in sheep. Journal of Endocrinology 165:625-637. crossref(new window)

Blanchard, M. M., C. G. Goodyear, J. Charrier and B. Barenton. 1988. In vitro regulation of growth hormone (GH) release from ovine pituitary cells during fetal and neonatal development: effects of GH-Releasing Factor, Somatostatin and Insulin-like Growth Factor 1. Endocrinology 122(5):2114-2120. crossref(new window)

Blum, W. F., P. Englaro, S. Hanitsch, A. Juul, N. T. Hertel, J. Muller, N. E. Skakkebaek, M. L. Heiman, M. Birkett, A. M. Attanasio, W. Kiess and W. Rascher. 1997. Plasma leptin levels in healthy children and adolescents: dependence on body mass index, body fat mass, gender, pubertal stage and testosterone. J. Clin. Endocrinol. & Metab. 82:2904-2910. crossref(new window)

Blum, W. F. and M. B. Ranke. 1991. Plasma IGFBP-3 levels as clinical indicators. In: Modern Concepts of Insulin-Like Growth Factor (Ed. Spencer EM). Elsevier, New York. pp. 381-393.

Bocquier, F., M. Bonnet, Y. Faulconnier, M. Guerre-Millo, P. Martin and Y. Chilliard. 1998. Effects of photoperiod and feeding level on perirenal adipose tissue metabolic activity and leptin synthesis in the ovariectomized ewe. Reproduction and Nutrition Development, 38:489-498. crossref(new window)

Cameron, N. D., J. C. Penman and E. McCullough. 2000. Serum leptin concentration in pigs selected for high or low daily food intake. Genetic Research, 75:209-213. crossref(new window)

Daughaday, W. H., I. K. Mariz and S. L. Blethen. 1980. Inhibition of assessment of bound somatomedin to membrane receptor and immunobinding sites: A comparison of radioreceptor and radioimmunoassay of somatomedin in native and acid –ethanol extraction serum. J. Clin. Endocrinol. & Metab. 5:781-788. crossref(new window)

Delavaud, C., F. Bocquier, Y. Chilliard, D. H. Keisler, A. Gertler and G. Kann. 2000. Plasma leptin determination in ruminants: effect of nutritional status and body fatness on plasma leptin concentration assessed by a specific RIA in sheep. Journal of Endocrinology 165:519-526. crossref(new window)

Dyer, C. J., J. M. Simmons, R. L. Matteri and D. H. Keisler. 1997. Leptin receptor mRNA is expressed in ewe anterior pituitary and adipose tissue and is deferentially expressed in hypothalamic regions of well-fed and feed-restricted ewes. Domest. Anim. Endocrinol. 14:119-128. crossref(new window)

Ehrhardt, R. A., R. M. Slepetis, J. Siegel-Willott, M. E. Van Amburgh, A. W. Bell and Y. R. Boisclair. 2000. Development of a specific radioimmunoassay to measure physiological changes of circulating leptin in cattle and sheep. Journal of Endocrinology 166:519-528. crossref(new window)

Estienne, M. J., A. F. Harper, C. R. Barb and M. J. Azain. 2000. Concentration of leptin in serum and milk collected from lactating sows differing in body condition. Domest. Anim. Endocrinol. 19:275-280. crossref(new window)

Houseknecht, K. L., C. P. Portocarrero, S. Ji, R. Lemenager and M. E. Spurlock. 2000. Growth hormone regulates leptin gene expression in bovine adipose tissue: correlation with adipose tissue: correlation with IGF-1 expression. Journal of Endocrinology 164:51-57. crossref(new window)

Kauter, K., M. Ball, P. Kearney, R. Tellam and J. R. McFarlane. 2000. Adrenaline, insulin and glucagons do not have acute effects on plasma leptin levels in sheep: development and characterization of an ovine leptin ELISA. Journal of Endocrinology 166:127-135. crossref(new window)

Lee, H-G, R. A. Vega RA, L. T. Phung, N. Matsunaga, H. Kuwayama and H. Hidari. 2000a. The effect of growth hormone-releasing peptide-2 (KP102) administration on plasma insulin-like growth factor (IGF)-1 and IGF-binding proteins in Holsteins steers on different planes of nutrition. Domest. Anim. Endocrinol. 18(3):293-301. crossref(new window)

Lee, H. G., R. A. Vega RA, L. T. Phung, N. Matsunaga, H. Kuwayama and H. Hidari. 2000b. Changes in plasma insulinlike growth factor (IGF) -1, IGF binding protein (GFBP) -2 and IGFBP-3 during fasting in Holstein adult steers and calves. Animal Science Journal (Jpn), 71(2):178-188.

Licinio, J., C. Mantzoros, A. B. Begrao, G. Cizza, M. Wong, P. B. Bongiorno, G. P. Chrousos, B. Karp, C. Allen, J. S. Flier and P. W. Gold. 1997. Human leptin levels are pulsatile and inversely related to pituitary-adrenal function. Nature Medicine, 3(5):575-79. crossref(new window)

Mantzoros, C. S., J. S. Flier and A. D. Rogol. 1997. A longitudinal assessment of hormonal and physical alterations during normal puberty in boys. V. Rising leptin levels may signal the onset of puberty. J. Clin. Endocrinol. & Metab. 82:1066-1070. crossref(new window)

McManus, C. J. and B. P. Fitzgerald. 2000. Effects of a single day of feed restriction on changes in serum leptin, gonadotropins, prolactin, and metabolites in aged and young mares. Domest. Anim. Endocrinol. 19:1-13. crossref(new window)

Morrison, C. D., J. A. Daniel, B. J. Holmberg, J. Djiane, N. Raver, A. Gertler and D. H. Keisler. 2001. Central infusion of leptin into well-fed and undernourished ewe lambs: effects of feed intake and serum concentration of growth hormone and luteinizing hormone. Journal of Endocrinology 168:317-324. crossref(new window)

Purchas, R. W., K. L. MacMillan and H. D. Hafs. 1970. Pituitary and plasma Growth Hormone levels in bulls from birth to one year of age. J. Anim. Sci. 31:358-363. crossref(new window)

Ravussin, E., R. E. Pratley, M. Maffei, H. Wang, J. M. Friedman, P. H. Bennet and C. Bogardus. 1997. Relative low plasma leptin concentrations precede weight gain in Pima Indians. Nature Medicine 3(2):238-240. crossref(new window)

Robert, C., M. Palin, N. Coulombe, C. Roberge, F. G. Silversides, B. F. Benkel, R. M. McKay and G. Pelletier. 1998. Backfat thickness in pigs is positively associated with leptin mRNA levels. Can. J. Anim. Sci. 78:473-482. crossref(new window)

SAS. 1988. SAS/STAT User's Guide (Release 6.03 Ed). SAS Inst., Inc., Cary, NC.

Spurlock, M. E., A. M. A. Ranalletta, S. G. Cornelius, G. R. Frank, G. M. Willis, S. Ji, A. L. Grant and C. A. Bidwell. 1998. Leptin expression in adipose tissue is not increased by endotoxin but is reduced by growth hormone. Journal of Interferon and Cytokine Research, 18:1051-1058. crossref(new window)

Takahashi, M., T. Funahashi, I. Shimomura, K. Miyaoka and Y. Matsuzawa. 1996. Plasma leptin levels and body fat distribution. Hormone Metabolic Research, 28:751-752. crossref(new window)

Urbanski, H. and K-Y. F. Pau. 1998. A biphasic developmental pattern of circulating leptin in the male rhesus macaque (Macaca mulata). Endocrinology 139(5):2284-2286. crossref(new window)