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Evaluation of Ascorbic Acid Treatment in Clinical and Subclinical Mastitis of Indian Dairy Cows
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 Title & Authors
Evaluation of Ascorbic Acid Treatment in Clinical and Subclinical Mastitis of Indian Dairy Cows
Naresh, Ram; Dwivedi, S.K.; Swarup, D.; Patra, R.C.;
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 Abstract
A study was carried out to assess the therapeutic effect of ascorbic acid in mastitis of dairy cows. The herd with a population of 250-275 lactating cows was screened for clinical and subclinical mastitis for a period of 5 months. Based on inclusion and exclusion criteria, eighteen animals each with clinical and subclinical mastitis in one quarter only were selected as study population. Twelve cows (group A) with normal udder and health were also selected as a healthy control. Clinical mastitis cows were grouped as B (n=12) and C (n=6). Cows of group B were treated with ascorbic acid at 25 mg/kg, subcutaneously for 5 consecutive days and intramammary infusion (Ampicillin sodium 75 mg and Cloxacillin sodium 200 mg/infusion) based on antibiotic sensitivity test, till complete recovery. Group C cows received only intramammary infusion till the complete recovery. Eighteen subclinical mastitis cows were divided in group D (n=12) and E (n=6). Cows of group D were treated with ascorbic acid at 25 mg/kg subcutaneously for 5 consecutive days while group E did not receive any treatment. California mastitis test (CMT), somatic cell count (SCC), physical changes of udder and milk were used to diagnose and classify the mastitis. Evaluation of the therapy was based on CMT score and physical changes of udder and milk. Sample size calculation was also performed but was not followed for control groups due to scarcity of cases. Adequate blinding was done when and where required to avoid the biases. Confounding variables like herd, age of the cow, stage of the lactation, season and geographical region were duly considered and adequate blocking was followed. Ascorbic acid was administered in clinical and subclinical cases even after cure considering its immunostimulatory and healing inducing effects. The recovery rate was faster in cases of clinical mastitis treated with ascorbic acid along with an intramammary infusion (group B) than the quarters of group C cows. Quarter wise the average duration/number (3.160.11 days) of antimicrobial intramammary infusion was significantly (p<0.01) less in group B than that of average duration/number (5.330.20 days) of group C. Subclinical mastitis cows treated with ascorbic acid showed 83.33% recovery while 16.77% did not respond to treatment till last day of study. Cows of group E (untreated) did not recovered from the mastitis. Subjective parameters viz. swelling, pain reflex of udder and physical changes in milk from quarter of ascorbic acid treated cows (group B) disappeared earlier than that of group C cows. It is concluded from this study that the ascorbic acid might be useful as an adjunct in case of clinical mastitis to get quick recovery with less number of intramammary infusions. High recovery rate in subclinical mastitis quarters of group D cows is appreciable and opens a new avenue to conduct further trials in a larger population in various field conditions. However, the pharmacology of ascorbic acid with particular reference to health of mammary gland needs to be investigated.
 Keywords
Ascorbic Acid;Clinical Mastitis;Dairy Cows;Subclinical Mastitis and Treatment;
 Language
English
 Cited by
 References
1.
Antila, P. and V. Antila. 1979. The effect of mastitis on riboflavin, vitamin C and cholesterol contents of cow's milk. Meijerrieteellinen Aikakauskirja. 37:23-32.

2.
Aseltine, M. 1991. Vitamin E and selenium supplementation can prevent mastitis in dairy cattle. Feedstuffs. 63:11-12.

3.
Basu, T. K. and C. J. Schorah. 1982. Vitamin C in health and disease. AVI Publishing Company, Connecticut.

4.
Coles, E. H. 1980. Veterinary Clinical Pathology (3rd edition), W.B. Saunders Company, Philadelphia.

5.
Craven, N. 1987. Efficacy and financial value of antibiotic treatment of bovine clinical mastitis during lactation. British Vet. J. 143:410-422.

6.
Craven, N. and J. C. Anderson. 1982. Experimental acute Staphylococcal mastitis in the mouse: The influence of pathological changes on the kinetics and therapeutic action of cloxacillin. J. Comp. Pathol. 92:579-588. crossref(new window)

7.
Cummins, K. A., C. J. Brunner and P. J. Tyler. 1992. Effect of dietary ascorbate, alphatocopherol and colostrum on immune function in calves of either sex. J. Dairy Sci. 75(Suppl.):274 (Abstr.).

8.
Curca, D. and T. C. Fotache. 1985. Ascorbic acid content in the liver and adrenal glands of cattle with parasitic liver disease. Lucrari Stiintifice, Instititul Agronomic 'Nicolae Balcescu' CMedicina Veterinara. 28:55-59.

9.
Davis, R. H., K. Y. Rosenthal, L. R. Cesario and G. R. Rouw. 1990. Vitamin C influence on localised adjuvant arthritis. J. Am. Pediatric Med. Association. 80:414-418.

10.
Hagg, W. 1985. Methods and practical importance of determining vitamin C in cattle in the past and present. 196 pp (VETCD 1973-1988).

11.
Hagg, W. and W. Hofmann. 1987. Vitamin C content in blood plasma and leukocytes of cattle. Tierarztliche Umschau 42:956-963.

12.
Hidiroglou, M., T. R. Batra, M. Ivan and F. Markham. 1995. Effect of supplementatal vitamin E and C on the immune response of calves. J. Dairy Sci. 78:1578-1583.

13.
Hill, A. W., A. L. Shears and K. G. Hiibit. 1978. In: Efficacy and Financial Value of Antibiotic Treatment of Bovine Clinical Mastitis during Lactation British (Ed. N. Craven). 1987. Vet. J. 143:410-423.

14.
Hoeben, D., C. Burvenich and R. Heyneman. 1997. Influence of antimicrobial agent on bactericidal activity of bovine milk polymorphonuclear leukocytes. Vet. Immunol. and Immunopathol. 56:271-282. crossref(new window)

15.
Johnston, R. B. and J. E. Lehmeyer. 1977. EMBO workshop on superoxide and superoxide dismutase, (Ed. A. M. Michelson, J. M. McCord and I. Fridovich). Academic Press London. p. 291.

16.
Kobiesy, M. A. and T. S. A. El-Ali. 1994. Effect of ascorbic acid on growth performance and some blood constituents of suckling buffalo calves. Assuit-Vet. Med. J. 44:21-22.

17.
Lewin, S. 1975. Vitamin C: Its molecular biology and medical potential. Academic Press, London. pp. 81-85.

18.
Lintner, T. J. and R. J. Eberhart. 1990. Effects of antibiotics on phagocytic recruitment, function, and morphology in bovine mammary gland during the early nonlactating period. AJVR. 51:533-542.

19.
Nickerson, S. C., M. J. Paape and A. M. Dulin. 1985. Effect of antibiotics and vehicles on bovine mammary polymorphonuclear leukocyte morpholphologic features, viability, and phagocytic activity in vitro. AJVR. 46:2259-2265.

20.
Niki, E. 1991. Vitamin C as an antioxidant. World Rev. Nutr. Diet. pp. 1-30.

21.
Paape, M. J., R. H. Miller and G. Ziv. 1991. Pharmacologic enhancement or suppression of phagocytosis by bovine neutrophils. AJVR. 52:363-366.

22.
Radostits, O. M., D. C. Blood and C. C. Gay. 1994. Veterinary Medicine. Bailliere Tindall, London.

23.
Roth, J. A. and M. L. Kaeberle. 1985. In vivo effect of ascorbic acid on neutrophil function in healthy and dexamethasone treated cattle. AJVR. 46:2434-2436.

24.
Schukken, Y. H. and H. A. Deluyker. 1995. Design of field trials for the evaluation of antibacterial products for therapy of bovine clinical mastitis. J. Vet. Pharmacol. Therapeutics 18:274-283. crossref(new window)

25.
Singh, P. J. and K. B. Singh. 1994. A study of economic losses due to mastitis in India. Indian J. Dairy Sci. 47:265-272.

26.
Snedecor, G. W. and W. G. Cocharan. 1967. Statistical Methods (sixth edition), Oxford and IBH, New Delhi. pp. 258-268.

27.
Steffert, I. J. 1993. Compositional changes in cows milk association with health problems. Bull. Milk Fat Flavour Forum pp. 119-125.

28.
Thomas, W. R. and P. G. Holt. 1978. Vitamin C and immunity on assessment of the evidence. Clin. Exp. Immunol. 32:370-379.

29.
Upton, P. 1988. Method for treating or preventing bovine mastitis. United States Patent, U.S., 4, 782, 048, p. 4.

30.
Zubay, G. 1993. Biochemistry, 3rd ed. Brown Publisher, Oxford, England, p. 483.