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Screening of Conjugated Linoleic Acid Producing Lactic Acid Bacteria from Fecal Samples of Healthy Babies
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 Title & Authors
Screening of Conjugated Linoleic Acid Producing Lactic Acid Bacteria from Fecal Samples of Healthy Babies
Ham, J.S.; In, Y.M.; Jeong, S.G.; Kim, J.G.; Lee, E.H.; Kim, H.S.; Yoon, S.K.; Lee, B.H.;
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This study was carried out to obtain conjugated linoleic acid (CLA) producing lactic acid bacteria for further study on the enzymes related to the production of CLA which has gained considerable attention and on the development as a probiotic culture. Total 34 lactic acid bacteria were isolated from 19 feces samples of healthy babies. CLA forming ability was measured spectrophotometrically by the modification of linoleate 12-cis, 11-trans-isomerase activity measuring method, and CLA of the cultures were extracted, methylated, and examined by HPLC analysis. CLA methyl ester of only one culture showing the highest value of CLA forming ability could be detected by HPLC analysis. The culture was found to be Gram positive, rods and catalase negative. It grows at but not at , and was identified to be Lactobacillus fermentum on the basis of the biochemical characteristics and the utilization of substrates. These results provide an efficient experimental method to screen CLA producing lactic acid bacteria.
Conjugated Linoleic;Acid;Lactic Acid Bacteria;Lactobacillus Fermentum;
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시판 요구르트 제품 중 Conjugated Linoleic Acid (CLA) 함량 분석 및 상업용 Dairy Starter에 의한 CLA 생산,이효구;권영태;강혜순;윤칠석;정재홍;김형국;김인환;정수현;

한국식품영양과학회지, 2004. vol.33. 8, pp.1343-1347 crossref(new window)
한국 유아에서 분리한 Bifidobacteria에 의한 발효유의 Conjugated Linoleic Acid (CLA) 함량 증가,이효구;권영태;강혜순;윤칠석;정재홍;김인환;정수현;

한국식품영양과학회지, 2004. vol.33. 8, pp.1348-1352 crossref(new window)
Brassart, D. and E. J. Schiffrin. 2000. Pre- and probiotics. In:Essentials of Functional Foods (Ed. M. K. Schmidl and T. P.Labuza). Aspen publishers, Inc. Gaithersburg, Maryland. pp.205-216.

Chin, S. F., W. Liu, J. M. Storkson, Y. L. Ha and M. W. Pariza.1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. of Food Comp. And Analysis 5:185-197. crossref(new window)

Chin, S. F., J. M. Storkson, W. Liu, K. J. Albright and M. W. Pariza. 1994. Conjugated linoleic acid (9,11- and 10,12-octadecadienoic acid) is produced in conventional but not germ-free rats fed linoleic acid. J. Nutr. 124:694-701.

De Man, J. C., M. Rogosa and M. E. Sharpe. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23:130-135

Fogerty, A. C., G. L. Ford and D. Svoronos. 1988. Octadeca-9,11-dienoic acid in food stuffs and in the lipids of human blood and breast milk. Nutr. Rep. Int. 38:937-944.

Ha, Y. L., N. K. Grimm and M. W. Pariza. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis 8:1881-1887. crossref(new window)

Huang, Y. C., L. O. Luedecke and T. D. Shultz. 1994. Effect of cheddar cheese consumption on plasma conjugated linoleic acid in men. Nutrition Res. 14(3):373-386. crossref(new window)

Ip, C., S. F. Chin, J. A. Scimeca and M. W. Pariza. 1991. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Res. 51:6118-6124.

Jiang, J., L. Bjorck and R. Fonden. 1998. Production of conjugated linoleic acid by dairy starter cultures. J. Appl. Microbiol. 85:95-102. crossref(new window)

Kepler, C. R. and S. B. Tove. 1969. Linoleate cis-12, trans-11 isomerase. In: Methods in Enzymology (Ed. J. M. Lowenstein). Vol. XIV. Academic Press, New York. pp. 105-110. crossref(new window)

Nichols, P. L., Jr., S. F. Herb and R. W. Riemenschneider. 1951. Isomers of conjugated linoleic acid. I. Alkali isomerized linoleic acid. J. Am. Chem. Soc. 73:247-252. crossref(new window)

Ogawa, J., K. Matsumura, S. Kishino, Y. Omura and S. Shimizu. 2001. Conjugated linoleic acid accumulation via 10-hydroxy-12-octadecaenoic acid during microaerobic transformation of linoleic acid by Lactobacillus acidophilus. Appl. and Environ. Microbiol. 67(3):1246-1252. crossref(new window)

Pariza, M. W. and W. A. Hargraves. 1985. A beef-derived mutagenesis modulator inhibits initiation of mouse epidermal tumors by 7, 12-dimethylbenz[a]anthracene. Carcinogenesis 6:591-593. crossref(new window)

Pariza, M. W. and X. Y. Yang. 2000. Method of producing conjugated fatty acids. US Patent 6,060,304.

Parodi, P. W. 1994. Conjugated linoleic acid-A anticarcinogenic fatty acid present in milk fat. Aust. J. Dairy Technol. 49(2):93-97.

Sehat, N., M. P. Yurawecz, J. A. G. Roach, M. M. Mossoba, J. K. G. Kramer and Y. Ku. 1998. Silver-ion high-performance liquid chromatographic separation and identification of conjugated linoleic acid isomers. Lipids 33:217-222. crossref(new window)

Yoon, C. S. 2001. Development and utilization of conjugated linoleic acid producing dairy starter cultures. In: Proceedings of the 28th Korean Society for Food Science of Animal Resources Symposium. pp. 89-97.