JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Antagonism against Helicobacter Pylori and Proteolysis of Lactobacillus Helveticus CU631 and Strain Identification
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Antagonism against Helicobacter Pylori and Proteolysis of Lactobacillus Helveticus CU631 and Strain Identification
Yoon, Y.H.; Won, B.R.;
  PDF(new window)
 Abstract
The antagonistic activities of 30 strains of lactobacilli against Helicobacter pylori were determined and Lactobacillus helveticus CU631 has been selected as the strain which possesses the strongest inhibitory effect in the disc diffusion assay showing inhibition zone diameter of , whereas those of L. plantarum and L. fermentum have been shown to be . H. pylori G88016 revealed the highest vacuolating toxin producing activity among the 8 strains, the inhibitory activity of L. helveticus CU631 in vacuolating toxin producing activity of H. pylori manifested in the co-culture of two strains and in the 5:5 mixture of supernatant of the two strains. Both L. helveticus CU631 and cell free culture supernatant had a strong inhibitory activities in urease and cytotoxin producing activities of H. pylori NCTC11637 and CJH12. An accelerated proteolytic activity of water soluble peptides by L. helveticus CU631 during the refrigeration storage has been manifested in the cream cheese. DNA seqences of 16S-23S ribosomal RNA spacer region showed typical pattern among the various strains of L. helveticus, which could be used in the identification of L. helveticus CU 631.
 Keywords
Antagonistic Activity;Lactobacilli;Helicobacter Pyroli;Urease;Vacuolatijg Toxicity;16S-23S R RNA Spacer Gene Sequencing;
 Language
English
 Cited by
1.
In vivo Antagonistic Effect of Lactobacillus helveticus CU 631 against Salmonella enteritidis KU101 infection,;;;

Asian-Australasian Journal of Animal Sciences, 2003. vol.16. 3, pp.430-434 crossref(new window)
2.
Lactobacillus spp의 Salmonella enteritidis KU 101에 대한 보호 효과와 L. casei YIT 9018의 16S-23S rRNA Intergenic Spacer Region 염기배열 특성,배진성;윤영호;

Journal of Animal Science and Technology, 2003. vol.45. 3, pp.473-482 crossref(new window)
3.
Purification and characterization of two bacteriocins from Lactobacillus brevis BK11 and Enterococcus faecalis BK61 showing anti-Helicobacter pylori activity,;

Applied Biological Chemistry, 2015. vol.58. 5, pp.703-714 crossref(new window)
 References
1.
Alatossava, T. and A. T. Timiskajarvi. 1997. Development of oligonucleotide, primers from the 16S-23S r RNA intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR. Intrnational J. Food Microbiol. 35:49-56. crossref(new window)

2.
Atherton, J. C., P. Cao, R. M. Peek, Jr., M. K. Tummuru, M. J. Blaserand and T. L. Cover. 1995. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific VacA types with cytotoxin pro duction and peptic ulceration. J. Biol. Chem. 270:17771-17777. crossref(new window)

3.
Bernet-Camard, M., V. Lievin, J. N. Brassart and A. Servin. 1997. The human Lactobacillus acidophilus strain LA1 secrets a nonbacteriocin antibacterial substance(s) active in vitro and in vivo. Appli. Environ. Microbiol. 63(7):2747-2753.

4.
Coconnier, M. H. and V. Lievin. 1998. Antagonistic activity against Helicobacter pylori infection in vitro and in vivo by the human Lactobacillus acidophilus strain LB. Appl. Enviromental Microbiol. 64(11):4573-4580.

5.
Cooper, K. E. The theory of antibiotic inhibition zone. 1964. In 'Analytical Microbiology' (Ed. F. Kavanaugh). Academic Press, Inc. New York. pp. 1-86.

6.
Cover, T. L. and M. J. Blaser. 1992. Helicobacter pylori and gastroduodenal disease. Ann. Rev. Med. 43:135-145. crossref(new window)

7.
Fuller, R. 1991. Probiotics in human medicine. 32:439-442.

8.
Gill, H. H. and H. G. Desai. 1993. J. Clinical Enterol. 16(1):6-9.

9.
Graham, D. Y., H. M. Malaty, D. G. Evans, D. J. Evans, P. D. Klein and E. Adam. 1991. Gastroenterol. 100:1495-1501.

10.
Heatly, R. V. 1995. The Helicobacter Handbook, Blackwell Science Ltd.

11.
Kamiya, S., M. Kai, A. Ozawa, H. Kobayashi, T. Shirai, S. Harasawa and T. Miwa. 1994. Characteristics of vacuolating toxin produced by Helicobacter pylori. European J. Gastroenterology & Hepatology. 6(suppl. 1):S23-27.

12.
Leunk, R. D., P. T. Johnson and B. C. David. 1988. Cytotoxin activity in broth-culture filtrates of Campylobacter pylori. J. Med Microviol. 26(2):93-94. crossref(new window)

13.
Midolo, P. D., J. R. Lambert, R. Hull, F. Luo and M. L. Grayson. 1995. In vitro inhibition of Helicobacter pylori NCTC11637 by organic acid and lactic acid bacteria. J. applied bacteriol. (79):475-479.

14.
Muehlenkamp-ulate, M. R. and J. J. Warthesen. 1999. Evaluation of Several Nonstarter Lactobacilli for Their Influence on Cheddar Cheese Slurry Proteolysis. J. Dairy Sci. 82:1370-1378.

15.
National Institute of Health. 1994. NIH Consensus Statement 12(1)

16.
Parker, R. B. 1974. Probiotics: The other half of the antibiotics story. Animal nutrition and Health. (29):4-8.

17.
Pagliaccia, C., de M. Bernard, P. Luoetti, X. Ji, D. Burroni, T. L. Cover, E. Papini, R. Rappuoli, J. L. Telford and J. M. Reyrat. 1998. The m2 form of Helicobacter pylori cytotoxin has cell type-specific vacuolating activity. Proc. Natl Acad. Sci. USA. 95:10212-102278. crossref(new window)

18.
Songa, Y., N. Kato, C. Liua, Y. Matsumiyaa H. Katob and K. Watanabe. 2000. Rapid identification of 11 human intestinal lactobacillus species by multiplex PCR assays using groupe - and species -specific primers derived from the 16S-23S rRNA intergenic spacer region and its flanking 23S rRNA. FEMS Micrbiol. Lett. 187(2):167-173.

19.
Streekumar, O. and A. Hosono. 2001. Antimutagenic Properties fo Fermented Milk. Asian-Aust. J. Anim. Sci.14:218-231.

20.
Wendakoon, C. N., W. Fedio, A. Macleod and L. Ozimek. 1998. In vitro inhibition of Helicobacter pylori by dairy stater cultures. Milchwissenschaft. Milk science international. 53(9):499-502.